A liquid medium permitting anaerobic growth of Neisseria gonorrhoeae

2009 ◽  
Vol 79 (3) ◽  
pp. 364-366 ◽  
Author(s):  
Jeremy James Wade ◽  
Michelle Angela Graver
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Liquid Medium ◽  
Anaerobic Growth

Different responses to nitrate and nitrite by the model organism Escherichia coli and the human pathogen Neisseria gonorrhoeae

2006 ◽  
Vol 34 (1) ◽  
pp. 111-114 ◽  
Author(s):  
R.N. Whitehead ◽  
J.A. Cole
Keyword(s):  
Escherichia Coli ◽  
Neisseria Gonorrhoeae ◽  
Model Organism ◽  
Anaerobic Growth ◽  
Human Pathogen ◽  
Regulate Gene Expression ◽  
E Coli ◽  
Regulatory Systems ◽  
Two Component ◽  
Nitrate And Nitrite

The ability of Escherichia coli to use both nitrate and nitrite as terminal electron acceptors during anaerobic growth is mediated by the dual-acting two-component regulatory systems NarX-NarL and NarQ-NarP. In contrast, Neisseria gonorrhoeae responds only to nitrite: it expresses only NarQ-NarP. We have shown that although N. gonorrhoeae NarQ can phosphorylate E. coli NarL and NarP, the N. gonorrhoeae NarP is unable to regulate gene expression in E. coli. Mutagenesis experiments have revealed residues in E. coli NarQ that are essential for nitrate and nitrite sensing. Chimaeric proteins revealed domains of NarQ that are important for ligand sensing.

scholarly journals Reversion of Kellogg's Colonial Types of Neisseria Gonorrhoeae In Liquid Medium

1977 ◽  
Vol 10 (3) ◽  
pp. 377-380 ◽  
Author(s):  
S. HAFIZ ◽  
M. G. MCENTEGART ◽  
A. E. JEPHCOTT
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Liquid Medium

scholarly journals Conditions required for the attainment of colony-type stability of Neisseria gonorrhoeae in liquid culture

1975 ◽  
Vol 2 (5) ◽  
pp. 387-390
Author(s):  
E J Hart ◽  
I D Goldberg
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Liquid Medium ◽  
Liquid Culture ◽  
Reliable Method ◽  
Prolonged Incubation ◽  
Colony Type ◽  
Colony Forming Units ◽  
Large Populations

Colony-type morphology in Neisseria gonorrhoeae is associated with virulence, transformability, and the presence or absence of pili. A reliable method for achieving large populations of cells that are relatively stable with respect to colony type would be valuable, for example, in studies of virulence or for the isolation of pilus-specific phages. Previously described methods designed to achieve type stability in liquid culture were inadequate for a variety of reasons, including their low final cell yields and/or their requirements for prolonged incubation. The success of the procedure described in this communication depends upon the use of an overnight plate harvest to insure a relatively large and stable inoculum for the liquid medium. Yields of as high as 10(10) colony-forming units/ml are routinely obtained after 4 to 5 h of incubation. Such cultures exhibit a colonial-type stability of 85 to 95% with respect to the original colonial type used for inoculation of the start plate.

scholarly journals Dense Cultures of Neisseria gonorrhoeae in Liquid Medium

1967 ◽  
Vol 15 (2) ◽  
pp. 219-223 ◽  
Author(s):  
R. Brookes ◽  
C.-G. Hedén
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Liquid Medium
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