Isolation and characterization of Acidithiobacillus caldus from a sulfur-oxidizing bacterial biosensor and its role in detection of toxic chemicals

Seventy bacterial isolates were recovered from different rusted iron materials using modified NP glucose, 9K and sulfur oxidizing media. Thirty four isolates were selected after primary screening on the basis of their growth in modified NP glucose medium. Out of 34 isolates 5 belonged to the genus Kurthia, 6 Thiobacillus, 16 Bacillus and remaining 7 were Acidiphilium, Oscillospira, Sulfobacillus, Alicyclobacillus, Neisseria, Pseudomonas and Rahnella. One of the isolated organisms, Bacillus megaterium (F16/2), was found to be thermophilic. The organisms showed better growth in presence of iron salts like ferrous sulfate, ferric sulfate and ammonium ferric citrate. Five isolates were found to transform ferrous (Fe2+) to ferric (Fe3+) of which Bacillus subtilis (F8) was most efficient. A good number of bacteria were associated with oxidation of iron. Key words: Isolation; Bacteria; Rusted iron; Transformation DOI: 10.3329/bjb.v39i2.7479 Bangladesh J. Bot. 39(2): 185-191, 2010 (December)

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Isolation and Characterization of Iron and Sulfur-Oxidizing Bacteria from Maiduk Copper Mine at Shahrbabk Province in Iran

Geomicrobiology Journal ◽

10.1080/01490451.2017.1348408 ◽

2018 ◽

Vol 35 (4) ◽

pp. 261-265 ◽

Cited By ~ 3

Author(s):

Mehdi Hassanshahian ◽

Somayeh Ghoebani

Keyword(s):

Copper Mine ◽

Isolation And Characterization ◽

Sulfur Oxidizing ◽

Sulfur Oxidizing Bacteria

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Isolation and characterization of Acidithiobacillus caldus from several typical environments in China

Journal of Central South University of Technology ◽

10.1007/s11771-007-0033-0 ◽

2007 ◽

Vol 14 (2) ◽

pp. 163-169 ◽

Cited By ~ 13

Author(s):

Hong-bo Zhou ◽

Xi Liu ◽

Bo Fu ◽

Guan-zhou Qiu ◽

Qiang Huo ◽

...

Keyword(s):

Acidithiobacillus Caldus ◽

Isolation And Characterization

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Isolation and characterization of nitrate reductase from the halophilic sulfur-oxidizing bacterium Thioalkalivibrio nitratireducens

Biochemistry (Moscow) ◽

10.1134/s000629791006009x ◽

2010 ◽

Vol 75 (6) ◽

pp. 744-751 ◽

Cited By ~ 8

Author(s):

A. A. Filimonenkov ◽

R. A. Zvyagilskaya ◽

T. V. Tikhonova ◽

V. O. Popov

Keyword(s):

Nitrate Reductase ◽

Isolation And Characterization ◽

Sulfur Oxidizing

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ISOLATION AND CHARACTERIZATION OF SULFUR-OXIDIZING BACTERIA (SOB) FROM WASTE OF RUBBER FACTORY AND PAPER IN JAMBI

International Journal of Ecophysiology ◽

10.32734/ijoep.v1i2.1276 ◽

2019 ◽

Vol 1 (2) ◽

pp. 131-139

Author(s):

Suparjo

Keyword(s):

Soil Fertility ◽

Biochemical Characterization ◽

Selective Medium ◽

High Concentration ◽

Isolation And Characterization ◽

Sulfur Bacteria ◽

Ph Reduction ◽

Sulfur Oxidizing ◽

Different Sources

Sewage contains high concentration of sulfate and contaminates ground water supply. The production process in the factory produced sewage containing high amount of sulfur such as sulphide and sulphate. Sulfur bacteria, which are capable of oxidizing, reducing or partially oxidizing inorganic sulfur compounds such as Thiomicrospira, Thiothrix, Desulforomonas, Achromatium, and a group of Thiobacillus. Utilization of sulfur bacteria has done in attemp to find sewage bioremediation agents as well as to promote soil fertility. Isolation and characterization were carried out by growing the bacteria in the starkey selective medium, including pH reduction test, morphology, physiology and biochemical characterization. It has been observed that out of 2 isolates from different sources of samples (rubber and paper sewage), only one isolates related to sulfur-oxidizing bacterium (SOB) while the isolates AE01 related to true sulfur-oxidizing bacterium in genus Thiobacillus.

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