An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS

2018 ◽  
Vol 148 ◽  
pp. 40-45 ◽  
Author(s):  
Sachio Tsuchida ◽  
Syota Murata ◽  
Akiko Miyabe ◽  
Mamoru Satoh ◽  
Masaki Takiwaki ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Bacterial Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Comparison of the Fully Automated FilmArray BCID Assay to a 4-Hour Culture Test Coupled to Mass Spectrometry for Day 0 Identification of Microorganisms in Positive Blood Cultures

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Paul O. Verhoeven ◽  
Cyrille H. Haddar ◽  
Josselin Rigaill ◽  
Nathalie Fonsale ◽  
Anne Carricajo ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Blood Stream Infection ◽  
Blood Stream ◽  
Bacterial Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Negative Results ◽  
Culture Identification ◽  
Tof Ms

Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture Identification (BCID) assay by comparison to an in-house protocol based on MALDI-TOF MS identification of microcolonies after a 4-hour culture, for identifying on the same day the microorganisms present in positive blood culture bottles. One hundred and fifty-three positive bottles from 123 patients were tested prospectively by the 3 techniques of bacterial identification: 11 bottles yielding negative results by the 3 tests were considered false positive (7.2%). The reference MALDI-TOF MS technique identified 134 monomicrobial (87.6%) and 8 double infections (5.2%), which resulted in a total of 150 microorganisms. Globally, 137 (91.3%) of these 150 pathogens were correctly identified by the fully automated multiplex FilmArray BCID system at the species or genus level on day of growth detection, versus 117 (78.8%) by MALDI-TOF MS identification on nascent microcolonies after a 4-hour culture (P < 0.01). By combining the two approaches, 140 (93.5%) of the positive bottles were identified successfully at day 0. These results confirm the excellent sensitivity of the FilmArray BCID assay, notably in case of multimicrobial infection. Due to the limited number of targets included into the test, it must be coupled to another identification strategy, as that presented in this study relying on MALDI-TOF MS identification of microcolonies obtained after a very short culture period.

Superiority of SDS lysis over saponin lysis for direct bacterial identification from positive blood culture bottle by MALDI-TOF MS

2016 ◽  
Vol 11 (5-6) ◽  
pp. 1600131 ◽  
Author(s):  
Yvan Caspar ◽  
Cécile Garnaud ◽  
Mariya Raykova ◽  
Sébastien Bailly ◽  
Marie Bidart ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Blood Culture Bottle ◽  
Bacterial Identification ◽  
Culture Bottle ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium

2016 ◽  
Vol 74 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Antonio Curtoni ◽  
Raffaella Cipriani ◽  
Elisa Simona Marra ◽  
Anna Maria Barbui ◽  
Rossana Cavallo ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Solid Medium ◽  
Rapid Identification ◽  
Short Term ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals MALDI-TOF MS based carbapenemase detection from culture isolates and from positive blood culture vials

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
B. Ghebremedhin ◽  
A. Halstenbach ◽  
M. Smiljanic ◽  
M. Kaase ◽  
P. Ahmad-Nejad
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media

2015 ◽  
Vol 64 (11) ◽  
pp. 1346-1352 ◽  
Author(s):  
Osman Altun ◽  
Silvia Botero-Kleiven ◽  
Sarah Carlsson ◽  
Måns Ullberg ◽  
Volkan Özenci
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Rapid Identification ◽  
Short Term ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Identification Of Bacteria ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document