Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media

Using MALDI-TOF MS directly from blood culture bottles reduces the time required for pathogen identification, and the turnaround times for final identification have been compared with overnight incubation from solid media in previous studies. However, identification from a short incubation of agar plates has been increasingly accepted and successfully implemented in routine laboratories, but there is no data comparing direct MALDI-TOF MS with the short-term, incubated agar plates.

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Rapid identification of bacteria from bioMerieux BacT/ALERT blood culture bottles by MALDI-TOF MS

British Journal of Biomedical Science ◽

10.1080/09674845.2013.11669949 ◽

2013 ◽

Vol 70 (4) ◽

pp. 149-155 ◽

Cited By ~ 23

Author(s):

J.D. Haigh ◽

I.M. Green ◽

D. Ball ◽

M. Eydmann ◽

M. Millar ◽

...

Keyword(s):

Blood Culture ◽

Rapid Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Identification Of Bacteria ◽

Tof Ms

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Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid identification of yeast from positive blood culture

Journal of Microbiological Methods ◽

10.1016/j.mimet.2014.06.018 ◽

2014 ◽

Vol 104 ◽

pp. 92-93 ◽

Cited By ~ 16

Author(s):

M. Paolucci ◽

C. Foschi ◽

M.V. Tamburini ◽

S. Ambretti ◽

T. Lazzarotto ◽

...

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Rapid Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Culture Identification ◽

Tof Ms

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Evaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Rapid Identification of Bacteria in Processed Soybean Products

Journal of Food Research ◽

10.5539/jfr.v2n3p104 ◽

2013 ◽

Vol 2 (3) ◽

pp. 104 ◽

Cited By ~ 2

Author(s):

Yuko Furukawa ◽

Mitsuru Katase ◽

Kazunobu Tsumura

Keyword(s):

Laser Desorption ◽

Rapid Identification ◽

Bacterial Cells ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Identification Of Bacteria ◽

Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently been demonstrated as a rapid and reliable method for identifying bacteria in colonies grown on culture plates. Rapid identification of food spoilage bacteria is important for ensuring the quality and safety of food. To shorten the time of analysis, several researchers have proposed the direct MALDI-TOF MS tequnics for identification of bacteria in clinical samples such as urine and positive blood cultures. In this study, processed soybean products (total 26 test samples) were initially conducted a culture enrichiment step and bacterial cells were separated from interfering components. Harvested bacterial cells were determined by MALDI-TOF MS and 16S rRNA gene sequencing method. Six processed soybean products (23%) were increased bacterial cells after culture enrichiment step and they were sucessfully obtained the accurate identification results by MALDI-TOF MS-based method without colony formation.

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