Nhiễm trùng huyết cấy máu dương tính ở bệnh nhân mắc Covid-19

TÓM TẮT Đặt vấn đề: Nhiễm Covid-19 đồng thời nhiễm trùng huyết là một bệnh nặng, nguy cơ tử vong cao. Nghiên cứu này nhằm mô tả đặc điểm bệnh nhân Covid-19 nhiễm trùng huyết cấy máu dương tính; đặc điểm vi khuẩn gây nhiễm trùng huyết và mức độ đáp ứng kháng sinh của vi khuẩn trên kháng sinh đồ. Phương pháp nghiên cứu: Mô tả cắt ngang, 26 bệnh nhân Covid-19 cấy máu dương tính từ 8/2021 - 10/2021. Kết quả: 26 bệnh nhân có độ tuổi trung bình 57,89 (nhỏ nhất 32; lớn nhất 78) tuổi, 57,7% bệnh nhân lớn hơn 60 tuổi, nữ nhiều hơn nam, 69,23% bệnh nhân có bệnh nền, thời gian nằm viện trung bình là 16,35 ngày, 84,62% bệnh nhân cấy máu dương tính sau 48 giờ nằm viện, 23,08% bệnh nhân hồi phục sau điều trị. 96,15% bệnh nhân tăng CRP, 100% bệnh nhân tăng Procalcitonin, 86,46% bệnh nhân tăng bạch cầu chung và bạch cầu trung tính. Vi khuẩn gây nhiễm trùng huyết nhiều nhất là Acinetobacter baumannii, Escherichia coli, Burkholderia cepacia với tỷ lệ 19,23% mỗi loại. Vi khuẩn Gram âm nhạy cảm nhiều nhất với nhóm kháng sinh Carbapenem, Aminoglycosid, vi khuẩn Gram dương nhạy cảm nhiều với kháng sinh Linezolid, Vancomycin. Kết luận: Nhiễm trùng huyết trên bệnh nhân Covid làm tăng tỷ lệ tử vong, xác định chủng vi khuẩn và điều trị kháng sinh tích cực phù hợp với từng chủng vi khuẩn là cần thiết. ABSTRACT POSITIVE BLOOD CULTURE SEPSIS IN COVID-19 PATIENTS Background: Infection with Covid 19 and sepsis is a severedisease that leads to a high risk of death. This study aims to describe the characteristics of Covid-19 patients with positive blood culture, elements of bacteria causing sepsis, and the level of antibiotic response of bacteria on the antibiotic chart. Methods: A cross - sectional descriptive study was conducted on 26 Covid-19 patients with positive blood culture 8/2021-10/2021. Result: Twenty - six Covid-19 patients had an average age of 57.89 years (range: 32 - 78). Of these, 57.7% of patients were older than 60 years; women were more than men. 69.23% of patients had a medical history of the disease. The average treatment time was 16.35 days; 84.62% of patients had positive blood cultures after 48 hours of treatment. 23.08% of patients were recovered after treatment. 96.15% of patients increased CRP, 100% of patientsincreased Procalcitonin, and 86.46% increased leukocytosis and neutrophils. The bacteria that caused the most sepsis are Acinetobacter baumannii, Escherichia coli, Burkholderia cepacia with 19.23%. Gram - negative bacteria were most sensitive to Carbapenem, Aminoglycoside antibiotics. Gram - positive bacteria were more susceptible to Linezolid and Vancomycin antibiotics. Conclusion: Sepsis with positive blood culture in covid patients increases the mortality rate. Identifying bacterial strains and appropriate aggressive antibiotic treatment for each bacterial is necessary. Keywords: Sepsis, bacteria, sensitive, Covid-19.

Clinical Significance of Staphylococcus aureus in a Single Positive Blood Culture Bottle

Clinical significance of a single positive blood culture bottle (SPBCB) with Staphylococcus aureus is unclear. We aimed to assess the significance of a SPBCB by looking at the associated outcomes. We performed a retrospective, multicenter study of patients with a SPBCB with S. aureus using data collected from both electronic health records and the clinical microbiology laboratory. Overall, 534 patients with S. aureus bacteremia were identified and 118 (22.1%) had a SPBCB. Among cases with a SPBCB, 106 (89.8%) were classified as clinically significant while 12 (10.2%) were considered contaminated or of unclear significance. A majority (92.4%) of patients received antibiotic therapy but patients with clinically significant bacteremia were treated with longer courses (25.9 vs 5.7 days, P<0.001). Significant differences in both frequency of echocardiography (65.1% vs 84.6%, P<0.001), and infective endocarditis diagnosis (3.8% vs 14.2%, P=0.002) were seen in those with a single positive blood culture bottle compared to those with multiple positive bottles. A longer hospital length of stay, and higher 90-day, 6-month, and 1-year mortality rates were seen in patients with multiple positive blood culture bottles. A SPBCB with S. aureus was common among our patients. While this syndrome has a more favorable prognosis as compared to those with multiple positive blood cultures, clinicians should remain concerned as it portends a risk of infective endocarditis and mortality.

C-reactive protein as a diagnostic marker of neonatal sepsis in association with blood culture a retrospective study at a tertiary care hospital

To evaluate the sensitivity and specificity of C-Reactive protein as a single diagnostic inflammatory biomarker of neonatal sepsis in association with the blood culture.In this study, we retrospectively reviewed the medical records of 330 neonates at a tertiary care hospital at Gurugram from Jan, 2015 to Dec, 2020. The study population included neonates <1month age. Neonates meeting the IPSC criteria (Sepsis 2.0)1 and with a positive culture were considered as neonates with proven sepsis. Neonates with congenital malformations and congenital infections associated with TORCH complex were excluded from the study.Of the 330 neonates screened for sepsis, 32 (10%) had a positive blood culture with raised CRP in 69 (21%) cases. Among the 32 cases with positive blood culture, CRP identified 29 cases. The sensitivity, specificity, positive predictive value, negative predictive values of CRP were 90.6%, 86.5%, 42% and 99% respectively. The area under the curve (AUC) for the CRP ROC analysis was 0.83 with sensitivity of 90.6% and specificity of 91.6% which showed CRP usefulness as the diagnostic inflammatory biomarker of neonatal sepsis.Prematurity (53%) in neonates was the most common risk factor associated with neonatal sepsis. Klebsiella pneumoniae 11 (34%) was the most common pathogen isolated with 73% susceptibility to Ciprofloxacin.C-Reactive protein was found to have a high diagnostic value in terms of sensitivity of 90.6% and specificity of 91.6% when 0.83 is used as a cut off point for diagnosis of neonatal sepsis. Therefore, CRP could be used as diagnostic inflammatory biomarker in resource poor settings.

Bacterial DNA detection in very preterm infants assessed for risk of early onset sepsis

Objectives The aim of this study is to evaluate the diagnostic ability of multiplex real-time polymerase chain reaction (PCR) in very preterm infants assessed for risk of early onset neonatal sepsis (EOS). Methods Prospective observational cohort study. Blood samples of preterm neonates ≤32 weeks of gestation were evaluated by commercial multiplex real-time PCR within 2 h after delivery. The definition of EOS was based on positive blood culture and clinical signs of infection or negative blood culture, clinical signs of infection and abnormal neonatal blood count and serum biomarkers. Results Among 82 subjects analyzed in the study, 15 had clinical or confirmed EOS. PCR was positive in four of these infants (including the only one with a positive blood culture), as well as in 15 of the 67 infants without sepsis (sensitivity 27%, specificity 78%). Out of 19 PCR positive subjects, Escherichia coli was detected in 12 infants (63%). Statistically significant association was found between vaginal E. coli colonization of the mother and E. coli PCR positivity of the neonate (p=0.001). No relationship was found between neonatal E. coli swab results and assessment findings of bacterial DNA in neonatal blood stream. Conclusions Multiplex real-time PCR had insufficient diagnostic capability for EOS in high risk very preterm infants. The study revealed no significant association between PCR results and the diagnosis of clinical EOS. Correlation between maternal vaginal swab results and positive PCR in the newborn needs further investigation to fully understand the role of bacterial DNA analysis in preterm infants.

High Mortality among Premature Neonates with Positive Blood Culture Neonatal Sepsis in a Tertiary Hospital, Tanzania: A Call for Action

Well-documented vital signs are key in the prediction of sepsis in low- and middle-income countries. We determined prevalence, associated factors, and outcomes of positive blood culture sepsis in premature neonates at Bugando Medical Centre Mwanza, Tanzania. Temperature, oxygen saturation, heart rate, respiratory rate, and random blood glucose were repeatedly recorded at admission, 8 h, and 24 h in all 250 neonates enrolled. Clinical and microbiological data were collected from patient records followed by descriptive data analysis. The mean age of the neonates was 3 ± 5.2 days, with the majority (90%) aged <10 days. The prevalence of positive blood culture sepsis was 21.2% (95% CI: 16.1–26.2). The fluctuation of the random blood glucose (RBG) (aOR = 1.34, 95% CI: (1.07–1.67), p = 0.010), low oxygen saturation (aOR = 0.94, 95% CI: (0.88–0.99), p = 0.031), premature rupture of membrane aOR = 4.28, 95% CI: (1.71–10.71), p = 0.002), gestational age < 34 weeks (aOR = 2.73, 95% CI: (1.20–6.24), p = 0.017), and home delivery (aOR = 3.90, 95% CI: (1.07–14.19), p = 0.039) independently predicted positive blood culture. Significantly more deaths were recorded in neonates with a positive blood culture than those with a negative blood culture (32.1% vs. 5.1%, p < 0.001). In limited-resource settings, clinicians should use the vital signs and clinical information to initiate timely sepsis treatment among preterm neonates to prevent deaths and other morbidities.