Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid identification of yeast from positive blood culture

2014 ◽  
Vol 104 ◽  
pp. 92-93 ◽  
Author(s):  
M. Paolucci ◽  
C. Foschi ◽  
M.V. Tamburini ◽  
S. Ambretti ◽  
T. Lazzarotto ◽  
...  
2016 ◽  
Vol 74 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Antonio Curtoni ◽  
Raffaella Cipriani ◽  
Elisa Simona Marra ◽  
Anna Maria Barbui ◽  
Rossana Cavallo ◽  
...  

2015 ◽  
Vol 64 (11) ◽  
pp. 1346-1352 ◽  
Author(s):  
Osman Altun ◽  
Silvia Botero-Kleiven ◽  
Sarah Carlsson ◽  
Måns Ullberg ◽  
Volkan Özenci

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Paul O. Verhoeven ◽  
Cyrille H. Haddar ◽  
Josselin Rigaill ◽  
Nathalie Fonsale ◽  
Anne Carricajo ◽  
...  

Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture Identification (BCID) assay by comparison to an in-house protocol based on MALDI-TOF MS identification of microcolonies after a 4-hour culture, for identifying on the same day the microorganisms present in positive blood culture bottles. One hundred and fifty-three positive bottles from 123 patients were tested prospectively by the 3 techniques of bacterial identification: 11 bottles yielding negative results by the 3 tests were considered false positive (7.2%). The reference MALDI-TOF MS technique identified 134 monomicrobial (87.6%) and 8 double infections (5.2%), which resulted in a total of 150 microorganisms. Globally, 137 (91.3%) of these 150 pathogens were correctly identified by the fully automated multiplex FilmArray BCID system at the species or genus level on day of growth detection, versus 117 (78.8%) by MALDI-TOF MS identification on nascent microcolonies after a 4-hour culture (P < 0.01). By combining the two approaches, 140 (93.5%) of the positive bottles were identified successfully at day 0. These results confirm the excellent sensitivity of the FilmArray BCID assay, notably in case of multimicrobial infection. Due to the limited number of targets included into the test, it must be coupled to another identification strategy, as that presented in this study relying on MALDI-TOF MS identification of microcolonies obtained after a very short culture period.


2018 ◽  
Vol 67 (9) ◽  
pp. 1253-1256 ◽  
Author(s):  
Michael Payne ◽  
Sylvie Champagne ◽  
Christopher Lowe ◽  
Victor Leung ◽  
Michelle Hinch ◽  
...  

2013 ◽  
Vol 70 (4) ◽  
pp. 149-155 ◽  
Author(s):  
J.D. Haigh ◽  
I.M. Green ◽  
D. Ball ◽  
M. Eydmann ◽  
M. Millar ◽  
...  

Author(s):  
Hazan Zengin Canalp ◽  
Banu Bayraktar

Using MALDI-TOF MS directly from blood culture bottles reduces the time required for pathogen identification, and the turnaround times for final identification have been compared with overnight incubation from solid media in previous studies. However, identification from a short incubation of agar plates has been increasingly accepted and successfully implemented in routine laboratories, but there is no data comparing direct MALDI-TOF MS with the short-term, incubated agar plates.


Author(s):  
B. Ghebremedhin ◽  
A. Halstenbach ◽  
M. Smiljanic ◽  
M. Kaase ◽  
P. Ahmad-Nejad

Sign in / Sign up

Export Citation Format

Share Document