Efficiency of the novel quenching-probe PCR method to detect 23S rRNA mutations in children with Mycoplasma pneumoniae infection

We have found that early corticosteroid therapy was effective for reducing morbidity during five Korea-wide epidemics. We evaluated the clinical and laboratory parameters of 56 children who received early corticosteroid treatment for pneumonia that was caused by macrolide-resistant Mycoplasma pneumoniae (M. pneumoniae) or macrolide-sensitive M. pneumoniae between July 2019 and February 2020. All subjects had dual positive results from a PCR assay and serological test, and received corticosteroids within 24–36 h after admission. Point mutation of residues 2063, 2064, and 2067 was identified in domain V of 23S rRNA. The mean age was 6.8 years and the male:female ratio was 1.2:1 (31:25 patients). Most of the subjects had macrolide-resistant M. pneumoniae (73%), and all mutated strains had the A2063G transition. No significant differences in clinical and laboratory parameters were observed between macrolide-resistant and macrolide-sensitive M. pneumoniae groups that were treated with early dose-adjusted corticosteroids. Higher-dose steroid treatment may be needed for patients who have fever that persists for >48 h or increased biomarkers such as lactate dehydrogenase concentration at follow-up despite a usual dose of steroid therapy.

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Molecular exploration for Mycoplasma amphoriforme, Mycoplasma fermentans and Ureaplasma spp. from patient samples previously investigated for Mycoplasma pneumoniae infection

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2021.06.021 ◽

2021 ◽

Author(s):

Safi U. Rehman ◽

Jessica Day ◽

Baharak Afshar ◽

Richard S. Rowlands ◽

Harriet Billam ◽

...

Keyword(s):

Mycoplasma Pneumoniae ◽

Mycoplasma Fermentans ◽

Ureaplasma Spp ◽

Mycoplasma Pneumoniae Infection

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Epidemiology of Mycoplasma pneumoniae infection in the British Isles, 1974-9

Journal of Infection ◽

10.1016/s0163-4453(80)91584-4 ◽

1980 ◽

Vol 2 (2) ◽

pp. 191-194 ◽

Cited By ~ 10

Author(s):

Norman D. Noah ◽

Anne M. Urquhart

Keyword(s):

Mycoplasma Pneumoniae ◽

British Isles ◽

Mycoplasma Pneumoniae Infection

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Early Confirmation of Mycoplasma pneumoniae Infection by Two Short-Term Serologic IgM Examination

Diagnostics ◽

10.3390/diagnostics11020353 ◽

2021 ◽

Vol 11 (2) ◽

pp. 353

Author(s):

Ha Eun Jeon ◽

Hyun Mi Kang ◽

Eun Ae Yang ◽

Hye Young Han ◽

Seung Beom Han ◽

...

Keyword(s):

Mycoplasma Pneumoniae ◽

Early Stage ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Short Term ◽

Serologic Tests ◽

Polymerase Chain ◽

Positive Correlations ◽

Patient Selection Bias ◽

Mycoplasma Pneumoniae Infection

The aim of the present study is to re-evaluate the clinical application of two-times serologic immunoglobulin M (IgM) tests using microparticle agglutination assay (MAA), an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assay in diagnosing Mycoplasma pneumoniae (MP) infection. A retrospective analysis of 62 children with MP pneumonia during a recent epidemic (2019–2020) was conducted. The MAA and ELISA immunoglobulin M (IgM) and IgG measurements were conducted twice at admission and around discharge, and MP PCR once at presentation. Diagnostic rates in each test were calculated at presentation and at discharge. The seroconverters were 39% (24/62) of patients tested by MAA and 29% (18/62) by ELISA. At presentation, the diagnostic positive rates of MAA, ELISA, and PCR tests were 61%, 71%, and 52%, respectively. After the second examination, the rates were 100% in both serologic tests. There were positive correlations between the titers of MAA and the IgM values of ELISA. The single serologic IgM or PCR tests had limitations to select patients infected with MP in the early stage. The short-term, paired IgM serologic tests during hospitalization can reduce patient-selection bias in MP infection studies.

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