Phosphagen kinase in Schistosoma japonicum: Characterization of its enzymatic properties and determination of its gene structure

2013 ◽  
Vol 188 (2) ◽  
pp. 91-98 ◽  
Author(s):  
Shinji Tokuhiro ◽  
Kouji Uda ◽  
Hiroko Yano ◽  
Mitsuru Nagataki ◽  
Blanca R. Jarilla ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Gene Structure ◽  
Enzymatic Properties ◽  
Phosphagen Kinase ◽  
Its Gene

scholarly journals Enzymatic Properties and Intracellular Localization of the Novel Trichoderma reesei β-Glucosidase BGLII (Cel1A)

2002 ◽  
Vol 68 (9) ◽  
pp. 4546-4553 ◽  
Author(s):  
Markku Saloheimo ◽  
Juha Kuja-Panula ◽  
Erkko Ylösmäki ◽  
Michael Ward ◽  
Merja Penttilä
Keyword(s):  
Trichoderma Reesei ◽  
Culture Supernatant ◽  
Carbon Catabolite Repression ◽  
Intracellular Localization ◽  
Enzymatic Properties ◽  
The Novel ◽  
Transglycosylation Activity ◽  
Activity Measurements ◽  
Its Gene

ABSTRACT This paper describes the characterization of an intracellular β-glucosidase enzyme BGLII (Cel1a) and its gene (bgl2) from the cellulolytic fungus Trichoderma reesei (Hypocrea jecorina). The expression pattern of bgl2 is similar to that of other cellulase genes known from this fungus, and the gene would appear to be under the control of carbon catabolite repression mediated by the cre1 gene. The BGLII protein was produced in Escherichia coli, and its enzymatic properties were analyzed. It was shown to be a specific β-glucosidase, having no β-galactosidase side activity. It hydrolyzed both cellotriose and cellotetraose. BGLII exhibited transglycosylation activity, producing mainly cellotriose from cellobiose and sophorose and cellobiose from glucose. Antibodies raised against BGLII showed the presence of the enzyme in T. reesei cell lysates but not in the culture supernatant. Activity measurements and Western blot analysis of T. reesei strains expressing bgl2 from a constitutive promoter further confirmed the intracellular localization of this β-glucosidase.

scholarly journals Purification of Bovine Lysosomal alpha-Mannosidase, Characterization of its Gene and Determination of two Mutations that Cause alpha-Mannosidosis

1997 ◽  
Vol 246 (2) ◽  
pp. 410-419 ◽  
Author(s):  
Ole K. Tollersrud ◽  
Thomas Berg ◽  
Peter Healy ◽  
Gry Evjen ◽  
Umayal Ramachandran ◽  
...  
Keyword(s):  
Its Gene

Characterization of Impressed Current Technique to Model Corrosion of Reinforcement in Concrete

2020 ◽  
Vol 11 (1) ◽  
pp. 93-99
Author(s):  
Abu Zakir Morshed ◽  
Sheikh Shakib ◽  
Tanzim Jahin
Keyword(s):  
Immersion Time ◽  
Chloride Content ◽  
Engineering Science ◽  
Coastal Regions ◽  
Faraday’S Law ◽  
Current Technique ◽  
Corrosion Cell ◽  
Impressed Current

Corrosion of reinforcement is an important durability concern for the structures exposed to coastal regions. Since corrosion of reinforcement involves long periods of time, impressed current technique is usually used to accelerate the corrosion of reinforcement in laboratories. Characterization of impressed current technique was the main focus of this research,which involved determination of optimum chloride content and minimum immersion time of specimens for which the application of Faraday’s law could be efficient. To obtain optimum chloride content, the electrolytes in the corrosion cell were prepared similar to that of concrete pore solutions. Concrete prisms of 200 mm by 200 mm by 300 mm were used to determine the minimum immersion time for saturation. It was found that the optimum chloride content was 35 gm/L and the minimum immersion time for saturation was 140 hours. Accounting the results, a modified expression based on Faraday’s law was proposed to calculate weight loss due to corrosion. Journal of Engineering Science 11(1), 2020, 93-99

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