scholarly journals Characterization of GDP-mannose pyrophosphorylase from Escherichia coli O157:H7 EDL933 and its broad substrate specificity

2005 ◽  
Vol 37 (1-6) ◽  
pp. 1-8 ◽  
Author(s):  
Yung-Hun Yang ◽  
Young-Bok Kang ◽  
Kwang-Won Lee ◽  
Tek-Hyung Lee ◽  
Sung-Soo Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Escherichia Coli O157 ◽  
Broad Substrate Specificity

scholarly journals Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

2010 ◽  
Vol 76 (12) ◽  
pp. 4096-4098 ◽  
Author(s):  
Tatiana N. Stekhanova ◽  
Andrey V. Mardanov ◽  
Ekaterina Y. Bezsudnova ◽  
Vadim M. Gumerov ◽  
Nikolai V. Ravin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Alcohol Dehydrogenase ◽  
Substrate Specificity ◽  
Organic Solvents ◽  
Short Chain ◽  
Chain Alcohol ◽  
Hyperthermophilic Archaeon ◽  
Broad Substrate Specificity ◽  
Short Chain Alcohol

ABSTRACT Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus, was expressed in Escherichia coli, purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.

scholarly journals Biochemical Characterization of the POM-1 Metallo-β-Lactamase from Pseudomonas otitidis

2014 ◽  
Vol 59 (3) ◽  
pp. 1755-1758 ◽  
Author(s):  
Luisa Borgianni ◽  
Filomena De Luca ◽  
Maria Cristina Thaller ◽  
Yunsop Chong ◽  
Gian Maria Rossolini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Substrate Specificity ◽  
Biochemical Characterization ◽  
Content Type ◽  
Broad Substrate Specificity ◽  
Catalytic Activities

ABSTRACTThe POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced byPseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced inEscherichia coliBL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.

scholarly journals Toxigenic properties and stx phage characterization of Escherichia coli O157 isolated from animal sources in a developing country setting

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Mahdia Rahman ◽  
Ashikun Nabi ◽  
Md Asadulghani ◽  
Shah M. Faruque ◽  
Mohammad Aminul Islam
Keyword(s):  
Escherichia Coli ◽  
Developing Country ◽  
Escherichia Coli O157
Sign in / Sign up

Export Citation Format

Share Document