Johne’s disease in cattle: an in vitro model to study early response to infection of Mycobacterium avium subsp. paratuberculosis using RNA-seq.

2017 ◽  
Vol 91 ◽  
pp. 259-271 ◽  
Author(s):  
Rosanna Marino ◽  
Rossana Capoferri ◽  
Simona Panelli ◽  
Giulietta Minozzi ◽  
Francesco Strozzi ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
In Vitro Model ◽  
Early Response ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Rna Seq ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Vitro Model

scholarly journals The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow

2011 ◽  
Vol 24 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Johannes L. Khol ◽  
Pablo J. Pinedo ◽  
Claus D. Buergelt ◽  
Laura M. Neumann ◽  
Walter Baumgartner ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Fluid Collection ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lymph Fluid ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Lymphatic Fluid ◽  
Elisa Result

The objective of the current study was to evaluate the feasibility of lymph collection from the bovine udder and to investigate if the lymphatic fluid might be of diagnostic value in cows infected with Mycobacterium avium subsp. paratuberculosis (MAP), the etiologic agent of paratuberculosis. Lymph fluid collection was attempted from 58 cows, and the reactions of the cows as well as the level of difficulty of the procedure were recorded in 56 animals. Lymph samples (51 in total) were tested for the presence of MAP by nested polymerase chain reaction. Collection of the lymphatic fluid caused no or mild signs of discomfort in 94.6% of the cows; in 51.8% of cows, lymphatic fluid was attained on the first attempt, while sample collection was unsuccessful in 12.1%. Mycobacterium avium subsp. paratuberculosis was detected in 43.1% of all lymph samples. The bacterium was present in 66.7% of cows with clinical Johne’s disease, in 42.8% of asymptomatic cows with a positive or suspicious enzyme-linked immunosorbent assay (ELISA) result in blood, and in 38.7% of cows with a negative ELISA result in blood. The present study shows that the procedure was well tolerated by most cows and can easily be performed on farm. The current report of the isolation of MAP from lymph fluid suggests that the present approach could be used for the early detection of Johne’s disease in cattle.

scholarly journals Complete Genome Sequence of the Telford Type S Strain of Mycobacterium avium subsp. paratuberculosis

2019 ◽  
Vol 8 (11) ◽  
Author(s):  
Rudiger Brauning ◽  
Karren Plain ◽  
Milan Gautam ◽  
Tonia Russell ◽  
C. Carolina Correa ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Mycobacterium Avium ◽  
Causative Agent ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Content Type ◽  
Mycobacterium Avium Subsp Paratuberculosis

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne’s disease (JD).

scholarly journals Rapid Expression of Mycobacterium avium subsp. paratuberculosis Recombinant Proteins for Antigen Discovery

2006 ◽  
Vol 14 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Lingling Li ◽  
Shirin Munir ◽  
John P. Bannantine ◽  
Srinand Sreevatsan ◽  
Sagarika Kanjilal ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Mycobacterium Avium ◽  
In Vitro Transcription ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Antigen Discovery ◽  
Diagnostic Assays ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Granulomatous Enteritis

ABSTRACT Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.

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