PEG-based autonomous capillary system with integrated microbead array for immunoassay

Solid, monodisperse aerosols of NaCl, KCl, and AgCl have been used in a helium jet capillary system to transport unstable nuclides of Cu, Zn, Ga, and Sb a distance of 110 m. Overall transport efficiencies of ~ 60% have been achieved.

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Comparative analysis of the anatomical structure of heartwood and sapwood selected Gymnocladus canadensis Lam. trees in Srpska Crnja

Archives of Biological Sciences ◽

10.2298/abs1103831v ◽

2011 ◽

Vol 63 (3) ◽

pp. 831-836

Author(s):

Dragica Vilotic ◽

Mirjana Sijacic-Nikolic ◽

Danijela Miljkovic ◽

Mirjana Ocokoljic ◽

M. Rebic

Keyword(s):

Comparative Analysis ◽

North America ◽

Anatomical Structure ◽

Microscopic Structure ◽

Capillary System

This paper shows the results obtained from the study of the macroscopic-microscopic structure (capillary system) in the growth stem of Gymnocladus canadensis Lam. originating from North America, which grows in ?Muzljanski rit? in the area of Srpska Crnja. Gymnocladus canadensis Lam. falls under the ring-porous species according to its porosity, with large tracheas in its early zone. The early zone trachea lumens, contained in the sapwood, reach dimensions of up to 160 ?m, while early zone trachea lumens in the growth stem rings of the sapwood reach dimensions of up to 120 ?m. Examination of the microscopic structure of this tree show good properties of the tree.

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A More Aggressive Breast Cancer Spheroid Model Coupled to an Electronic Capillary Sensor System for a High-Content Screening of Cytotoxic Agents in Cancer Therapy: 3-Dimensional In Vitro Tumor Spheroids as a Screening Model

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057105277841 ◽

2005 ◽

Vol 10 (7) ◽

pp. 705-714 ◽

Cited By ~ 26

Author(s):

P. Bartholomä ◽

Impidjati ◽

A. Reininger-Mack ◽

Zhihong Zhang ◽

H. Thielecke ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Therapy ◽

Real Time ◽

Cytotoxic Agents ◽

High Content Screening ◽

Expression Vectors ◽

Tumor Spheroids ◽

Capillary System ◽

Cdna Expression

One major problem in cancer therapy is the immortality of tumor cells showing an active telomerase, which is responsible for the elongation of the telomeres after each cellular division and the knocking down of apoptotic suppressors. A further phenomenon occurring during cancer therapies is the problem of multicellular resistance. To develop therapeutic anticancer approaches inducing cellular apoptosis, gene-modified biological in vitro systems were established and evaluated for drug screening in a capillary system for a real-time, impedimertic monitoring. Multicellular spheroids of the human breast cancer cell line T-47D clone 11 were transfected with 1) antisense caspase-3 cDNA expression vectors for knocking down the main cell death molecule and 2) sense Bcl-xl cDNA expression vectors for overexpressing the apoptotic suppressor, resulting in more aggressive tumor models. These gene-modified tumor spheroids less sensitive for apoptosis were developed for screening drugs such as methotrexate in tumor spheroid–based biosensor systems via impedance spectroscopy. In this report, it is demonstrated that this could successfully exhibit that this real-time monitoring system with tumor spheroids positioned in a capillary system with a 4-electrode configuration is the most efficient high-content screening module for impedimetric measurements of physiological alterations during gene modification and drug application.

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