scholarly journals The use of debrided human articular cartilage for autologous chondrocyte implantation: Maintenance of chondrocyte differentiation and proliferation in type I collagen gels

2004 ◽  
Vol 22 (2) ◽  
pp. 446-455 ◽  
Author(s):  
Kanda Chaipinyo ◽  
Barry W. Oakes ◽  
Marie-Paule I. Van Damme
Keyword(s):  
Articular Cartilage ◽  
Autologous Chondrocyte Implantation ◽  
Type I Collagen ◽  
Chondrocyte Differentiation ◽  
Type I ◽  
Human Articular Cartilage ◽  
Collagen Gels ◽  
Chondrocyte Implantation ◽  
Differentiation And Proliferation ◽  
Autologous Chondrocyte

Histologic and Immunohistochemical Characteristics of Failed Articular Cartilage Resurfacing Procedures for Osteochondritis of the Knee

2007 ◽  
Vol 36 (2) ◽  
pp. 360-368 ◽  
Author(s):  
Robert F. LaPrade ◽  
Laura S. Bursch ◽  
Erik J. Olson ◽  
Vojtech Havlas ◽  
Cathy S. Carlson
Keyword(s):  
Articular Cartilage ◽  
Autologous Chondrocyte Implantation ◽  
Type I Collagen ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Repair Tissue ◽  
Tissue Area ◽  
Chondrocyte Implantation ◽  
Autologous Chondrocyte

Background The histologic appearance of the repair tissue after articular cartilage resurfacing procedures in humans is not well documented. Hypothesis The histologic and immunohistochemical appearance of the repair tissues in failed articular cartilage resurfacing procedures will be similar, regardless of the procedure that was done, and will not resemble normal articular cartilage. Study Design Case series; Level of evidence, 4. Methods Graft tissue from 10 patients who underwent an autologous chondrocyte implantation (n = 6), microfracture (n = 3), or periosteal transplantation (n = 1) procedure to treat symptomatic osteochondritis dissecans of the medial femoral condyle was processed for histologic examination after failure of the articular cartilage resurfacing procedure. Serial sections from all slabs were stained with hematoxylin and eosin and toluidine blue and were immunostained using antibodies directed against types I, II, and X collagen. Results Specimens from all 3 types of repair procedures were composed primarily of fibrous connective tissue and fibrocartilage. None of the sections stained positively for type X collagen. All 10 cases stained positively for type I collagen (range, 7%–97% of tissue area). Staining for type II collagen was positive in 4 of 6 autologous chondrocyte implantation cases, 3 of 3 microfracture cases, and the periosteal transplant case (range, 2%–65% of tissue area). In 8 of 10 cases, the percentage of the section area exhibiting positive staining for type I collagen was higher than for type II collagen (6 of 6 autologous chondrocyte implantation; 1 of 3 microfracture; 1 periosteal transplant). Conclusion The histologic appearance of the repair tissue of 3 different failed articular cartilage resurfacing procedures was similar and did not resemble normal articular cartilage.

A Prospective Multicenter Study on the Outcome of Type I Collagen Hydrogel–Based Autologous Chondrocyte Implantation (CaReS) for the Repair of Articular Cartilage Defects in the Knee

2011 ◽  
Vol 39 (12) ◽  
pp. 2558-2565 ◽  
Author(s):  
Ulrich Schneider ◽  
Lars Rackwitz ◽  
Stefan Andereya ◽  
Sebastian Siebenlist ◽  
Florian Fensky ◽  
...  
Keyword(s):  
Treatment Satisfaction ◽  
Type I Collagen ◽  
Type I ◽  
Prospective Multicenter Study ◽  
Collagen Hydrogel ◽  
Sf 36 ◽  
Chondrocyte Implantation ◽  
Ikdc Score ◽  
Autologous Chondrocyte

Background: The Cartilage Regeneration System (CaReS) is a novel matrix-associated autologous chondrocyte implantation (ACI) technique for the treatment of chondral and osteochondral lesions (Outerbridge grades III and IV). For this technology, no expansion of the chondrocytes in a monolayer culture is needed, and a homogeneous cell distribution within the gel is guaranteed. Purpose: To report a prospective multicenter study of matrix-associated ACI of the knee using a new type I collagen hydrogel (CaReS). Study Design: Case series; Level of evidence, 4. Methods: From 2003 to 2008, 116 patients (49 women and 67 men; mean age, 32.5 ± 8.9 years) had CaReS implantation of the knee in 9 different centers. On the basis of the International Cartilage Repair Society (ICRS) Cartilage Injury Evaluation Package 2000, the International Knee Documentation Committee (IKDC) score, pain score (visual analog scale [VAS]), SF-36 score, overall treatment satisfaction and the IKDC functional status were evaluated. Patient follow-up was performed at 3, 6, and 12 months after surgery and annually thereafter. Mean follow-up was 30.2 ± 17.4 months (range, 12-60 months). There were 67 defects of the medial condyle, 14 of the lateral, 22 of the patella/trochlea, and 3 of the tibial plateau, and 10 patients had 2 lesions. The mean defect size was 5.4 ± 2.4 cm2. Thirty percent of the defects were <4 cm2 and 70% were >4 cm2. Results: The IKDC score improved significantly from 42.4 ± 13.8 preoperatively to 70.5 ± 18.7 ( P < .001) at latest follow-up. Global pain level significantly decreased ( P < .001) from 6.7 ± 2.2 preoperatively to 3.2 ± 3.1 at latest follow-up. There also was a significant increase of both components of the SF-36 score. The overall treatment satisfaction was judged as very good or good in 88% by the surgeon and 80% by the patient. The IKDC functional knee status was grade I in 23.4%, II in 56.3%, III in 17.2%, and IV in 3.1% of the patients. Conclusion: Matrix-associated ACI employing the CaReS technology for the treatment of chondral or osteochondral defects of the knee is a safe and clinically effective treatment that yields significant functional improvement and improvement in pain level. However, further investigation is necessary to determine the long-term viability and clinical outcome of this procedure.

scholarly journals High-Density Autologous Chondrocyte Implantation as Treatment for Ankle Osteochondral Defects

Cartilage ◽  
2019 ◽  
pp. 194760351983589 ◽  
Author(s):  
Juan Manuel López-Alcorocho ◽  
Isabel Guillén-Vicente ◽  
Elena Rodríguez-Iñigo ◽  
Ramón Navarro ◽  
Rosa Caballero-Santos ◽  
...  
Keyword(s):  
Cartilage Repair ◽  
Autologous Chondrocyte Implantation ◽  
Histological Study ◽  
High Density ◽  
Osteochondral Lesions ◽  
Type I ◽  
Cartilage Lesions ◽  
Chondrocyte Implantation ◽  
Autologous Chondrocyte

Purpose Two-year follow-up to assess efficacy and safety of high-density autologous chondrocyte implantation (HD-ACI) in patients with cartilage lesions in the ankle. Design Twenty-four consecutive patients with International Cartilage repair Society (ICRS) grade 3-4 cartilage lesions of the ankle were included. Five million chondrocytes per cm2 of lesion were implanted using a type I/III collagen membrane as a carrier and treatment effectiveness was assessed by evaluating pain with the visual analogue scale (VAS) and American Orthopaedic Foot & Ankle Society (AOFAS) ankle-hindfoot score at baseline, 12-month, and 24-month follow-up, together with dorsal and plantar flexion. Magnetic resonance observation for cartilage repair tissue (MOCART) score was used to evaluate cartilage healing. Histological study was possible in 5 cases. Results Patients’ median age was 31 years (range 18-55 years). Median VAS score was 8 (range 5-10) at baseline, 1.5 (range 0-8) at 12-month follow-up, and 2 (rang e0-5) at 24-month follow-up ( P < 0.001). Median AOFAS score was 39.5 (range 29-48) at baseline, 90 (range 38-100) at 12-month follow-up, and 90 (range 40-100) at 24-month follow-up ( P < 0.001). Complete dorsal flexion significantly increased at 12 months (16/24, 66.7%) and 24 months (17/24, 70.8%) with regard to baseline (13/24, 54.2%) ( P = 0.002). MOCART at 12- and 24-month follow-ups were 73.71 ± 15.99 and 72.33 ± 16.21. Histological study confirmed that neosynthetized tissue was cartilage with hyaline extracellular matrix and numerous viable chondrocytes. Conclusion HD-ACI is a safe and effective technique to treat osteochondral lesions in the talus, providing good clinical and histological results at short- and mid-term follow-ups.

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