Baculovirus expression, purification, and characterization of human protein phosphatase 2A catalytic subunits α and β

2006 ◽  
Vol 45 (1) ◽  
pp. 150-156 ◽  
Author(s):  
Tsuyoshi Ikehara ◽  
Fukiko Shinjo ◽  
Satsuki Ikehara ◽  
Shihoko Imamura ◽  
Takeshi Yasumoto
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Human Protein ◽  
Purification And Characterization ◽  
Catalytic Subunits ◽  
Baculovirus Expression ◽  
Phosphatase 2A

scholarly journals Purification and Characterization of Protein Phosphatase 2A from Petals of the Tulip Tulipa gesnerina

BMB Reports ◽  
2006 ◽  
Vol 39 (6) ◽  
pp. 671-676 ◽  
Author(s):  
Md. Abul Kalam Azad ◽  
Yoshihiro Sawa ◽  
Takahiro Ishikawa ◽  
Hitoshi Shibata
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Purification And Characterization ◽  
Phosphatase 2A

scholarly journals The Role of Protein Phosphatase 2A Catalytic Subunit Cα in Embryogenesis: Evidence from Sequence Analysis and Localization Studies

1999 ◽  
Vol 380 (9) ◽  
pp. 1117-1120 ◽  
Author(s):  
Jürgen Götz ◽  
Wilfried Kues
Keyword(s):  
Protein Phosphatase ◽  
Genomic Organization ◽  
Protein Phosphatase 2A ◽  
Catalytic Subunit ◽  
Regulatory Subunit ◽  
Catalytic Subunits ◽  
Regulatory Subunits ◽  
Phosphatase 2A ◽  
The Brain

AbstractProtein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various sizes to form different heterotrimers which have been purified and evaluated with regard to substrate specificity. In fully differentiated tissues PP2A expression levels are highest in the brain, however, relatively little is known about expression in the developing embryo.In order to determine the composition of PP2A catalytic subunits in the mouse, cDNAs were cloned and the genomic organization of PP2A Cα was determined.By a gene targeting approach in the mouse, we have previously shown that the absence of the major catalytic subunit of PP2A, Cα, resulted in embryonic lethality around embryonic day E6.5. No mesoderm was formed which implied that PP2A plays a crucial role in gastrulation.Here, we extended our studies and analyzed wildtype embryos for Cα expression at subsequent stages of development. After gastrulation is completed, we find high expression of Cα restricted to the neural folds, which suggests that PP2A plays an additional pivotal role in neurulation.

Characterization of a cDNA encoding the 55 kDa B regulatory subunit of Arabidopsis protein phosphatase 2A

1995 ◽  
Vol 28 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Sabine J. Rundle ◽  
Andrew J. Hartung ◽  
John W. Corum ◽  
Michael O'Neill
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Regulatory Subunit ◽  
Arabidopsis Protein ◽  
Phosphatase 2A
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