Large scale preparation of recombinant human parathyroid hormone 1–84 from Escherichia coli

2007 ◽  
Vol 54 (2) ◽  
pp. 212-219 ◽  
Author(s):  
Qinghai Liu ◽  
Jinping Lin ◽  
Meiyun Liu ◽  
Xinyi Tao ◽  
Dongzhi Wei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Parathyroid Hormone ◽  
Large Scale ◽  
Human Parathyroid Hormone ◽  
Large Scale Preparation ◽  
Scale Preparation

Large-scale preparation and biological activity of recombinant human parathyroid hormone

1995 ◽  
Vol 39 (2) ◽  
pp. 129-136 ◽  
Author(s):  
J. Paulsen ◽  
D. Ochs ◽  
M. Harder ◽  
C. Duvos ◽  
H. Mayer ◽  
...  
Keyword(s):  
Biological Activity ◽  
Parathyroid Hormone ◽  
Large Scale ◽  
Human Parathyroid Hormone ◽  
Large Scale Preparation ◽  
Scale Preparation

An efficient and large-scale preparation process for polysialic acid by Escherichia coli CCTCC M208088

2010 ◽  
Vol 53 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Jin-Long Liu ◽  
Xiao-Bei Zhan ◽  
Jian-Rong Wu ◽  
Chi-Chung Lin ◽  
Dan-Feng Yu
Keyword(s):  
Escherichia Coli ◽  
Large Scale ◽  
Polysialic Acid ◽  
Preparation Process ◽  
Large Scale Preparation ◽  
Scale Preparation

Large-scale preparation of recombinant human calcitonin from a multimeric fusion protein produced in Escherichia coli

1999 ◽  
Vol 87 (3) ◽  
pp. 296-301 ◽  
Author(s):  
Hirokazu Ishikawa ◽  
Junko Kawaguchi ◽  
Yoshio Yao ◽  
Hidetsune Tamaoki ◽  
Takeshi Ono ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Large Scale ◽  
Human Calcitonin ◽  
Large Scale Preparation ◽  
Scale Preparation

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

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