Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2

2022 ◽  
Vol 189 ◽  
pp. 105986
Author(s):  
Qiang Yang ◽  
Zhiming Zheng ◽  
Genhai Zhao ◽  
Li Wang ◽  
Han Wang ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Elizabethkingia Meningoseptica

scholarly journals Characterization of Potential Drug Targets Farnesyl Diphosphate Synthase and Geranylgeranyl Diphosphate Synthase in Schistosoma mansoni

2013 ◽  
Vol 57 (12) ◽  
pp. 5969-5976 ◽  
Author(s):  
Peter D. Ziniel ◽  
Janish Desai ◽  
Cynthia L. Cass ◽  
Craig Gatto ◽  
Eric Oldfield ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Drug Targets ◽  
Protein Characterization ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Potential Drug ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Potential Drug Targets

ABSTRACTSchistosomiasis affects over 200 million people worldwide, with over 200,000 deaths annually. Currently, praziquantel is the only drug available against schistosomiasis. We report here thatSchistosoma mansonifarnesyl diphosphate synthase (SmFPPS) and geranylgeranyl diphosphate synthase (SmGGPPS) are potential drug targets for the treatment of schistosomiasis. We expressed active, recombinantSmFPPS andSmGGPPS for subsequent kinetic characterization and testing against a variety of bisphosphonate inhibitors. RecombinantSmFPPS was found to be a soluble 44.2-kDa protein, whileSmGGPPS was a soluble 38.3-kDa protein. Characterization of the substrate utilization of the two enzymes indicates that they have overlapping substrate specificities. AgainstSmFPPS, several bisphosphonates had 50% inhibitory concentrations (IC50s) in the low micromolar to nanomolar range; these inhibitors had significantly less activity againstSmGGPPS. Several lipophilic bisphosphonates were active againstex vivoadult worms, with worm death occurring over 4 to 6 days. These results indicate that FPPS and GGPPS could be of interest in the context of the emerging resistance to praziquantel in schistosomiasis therapy.

scholarly journals Isolation and characterization of Panax ginseng geranylgeranyl-diphosphate synthase genes responding to drought stress

2015 ◽  
Vol 142 (4) ◽  
pp. 747-758 ◽  
Author(s):  
Shadi Rahimi ◽  
Yu-Jin Kim ◽  
Balusamy Sri Renuka Devi ◽  
Altanzul Khorolragchaa ◽  
Johan Sukweenadhi ◽  
...  
Keyword(s):  
Drought Stress ◽  
Panax Ginseng ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Isolation And Characterization

Cloning, expression and characterization of an insect geranylgeranyl diphosphate synthase from Choristoneura fumiferana

2013 ◽  
Vol 43 (10) ◽  
pp. 947-958 ◽  
Author(s):  
Aline Barbar ◽  
Manon Couture ◽  
Stephanie E. Sen ◽  
Catherine Béliveau ◽  
Audrey Nisole ◽  
...  
Keyword(s):  
Choristoneura Fumiferana ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate
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