ScGST3 and multiple R2R3-MYB transcription factors function in anthocyanin accumulation in Senecio cruentus

Plant Science ◽  
2021 ◽  
pp. 111094
Author(s):  
Yumeng Cui ◽  
Jiawei Fan ◽  
Chenfei Lu ◽  
Jiangshan Ren ◽  
Fangting Qi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Anthocyanin Accumulation ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals Identification of Two Novel R2R3-MYB Transcription factors, PsMYB114L and PsMYB12L, Related to Anthocyanin Biosynthesis in Paeonia suffruticosa

2019 ◽  
Vol 20 (5) ◽  
pp. 1055 ◽  
Author(s):  
Xinpeng Zhang ◽  
Zongda Xu ◽  
Xiaoyan Yu ◽  
Lanyong Zhao ◽  
Mingyuan Zhao ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Anthocyanin Biosynthesis ◽  
Critical Role ◽  
Flower Color ◽  
Anthocyanin Accumulation ◽  
Paeonia Suffruticosa ◽  
Myb Transcription Factors ◽  
Transgenic Lines ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

Flower color is a charming phenotype with very important ornamental and commercial values. Anthocyanins play a critical role in determining flower color pattern formation, and their biosynthesis is typically regulated by R2R3-MYB transcription factors (TFs). Paeonia suffruticosa is a famous ornamental plant with colorful flowers. However, little is known about the R2R3-MYB TFs that regulate anthocyanin accumulation in P. suffruticosa. In the present study, two R2R3-MYB TFs, namely, PsMYB114L and PsMYB12L, were isolated from the petals of P. suffruticosa ‘Shima Nishiki’ and functionally characterized. Sequence analysis suggested that PsMYB114L contained a bHLH-interaction motif, whereas PsMYB12L contained two flavonol-specific motifs (SG7 and SG7-2). Subsequently, the in vivo function of PsMYB114L and PsMYB12L was investigated by their heterologous expression in Arabidopsis thaliana and apple calli. In transgenic Arabidopsis plants, overexpression of PsMYB114L and of PsMYB12L caused a significantly higher accumulation of anthocyanins, resulting in purple-red leaves. Transgenic apple calli overexpressing PsMYB114L and PsMYB12L also significantly enhanced the anthocyanins content and resulted in a change in the callus color to red. Meanwhile, gene expression analysis in A. thaliana and apple calli suggested that the expression levels of the flavonol synthase (MdFLS) and anthocyanidin reductase (MdANR) genes were significantly downregulated and the dihydroflavonol 4-reductase (AtDFR) and anthocyanin synthase (AtANS) genes were significantly upregulated in transgenic lines of PsMYB114L. Moreover, the expression level of the FLS gene (MdFLS) was significantly downregulated and the DFR (AtDFR/MdDFR) and ANS (AtANS/MdANS) genes were all significantly upregulated in transgenic lines plants of PsMYB12L. These results indicate that PsMYB114L and PsMYB12L both enhance anthocyanin accumulation by specifically regulating the expression of some anthocyanin biosynthesis-related genes in different plant species. Together, these results provide a valuable resource with which to further study the regulatory mechanism of anthocyanin biosynthesis in P. suffruticosa and for the breeding of tree peony cultivars with novel and charming flower colors.

New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in the flowers of transgenic tobacco

2015 ◽  
Vol 90 (1-2) ◽  
pp. 63-76 ◽  
Author(s):  
J. Ricardo Pérez-Díaz ◽  
Jorge Pérez-Díaz ◽  
José Madrid-Espinoza ◽  
Enrique González-Villanueva ◽  
Yerko Moreno ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transgenic Tobacco ◽  
Anthocyanin Accumulation ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals Novel R2R3-MYB transcription factors fromPrunus americanaregulate differential patterns of anthocyanin accumulation in tobacco and citrus

2017 ◽  
Vol 8 (2) ◽  
pp. 85-105 ◽  
Author(s):  
Kasturi Dasgupta ◽  
Roger Thilmony ◽  
Ed Stover ◽  
Maria Luiza Oliveira ◽  
James Thomson
Keyword(s):  
Transcription Factors ◽  
Anthocyanin Accumulation ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals Multiple R2R3-MYB Transcription Factors Involved in the Regulation of Anthocyanin Accumulation in Peach Flower

2016 ◽  
Vol 7 ◽  
Author(s):  
Hui Zhou ◽  
Qian Peng ◽  
Jianbo Zhao ◽  
Albert Owiti ◽  
Fei Ren ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Anthocyanin Accumulation ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals A Complex Interplay of Three R2R3 MYB Transcription Factors Determines the Profile of Aliphatic Glucosinolates in Arabidopsis

2010 ◽  
Vol 153 (1) ◽  
pp. 348-363 ◽  
Author(s):  
Ida Elken Sønderby ◽  
Meike Burow ◽  
Heather C. Rowe ◽  
Daniel J. Kliebenstein ◽  
Barbara Ann Halkier
Keyword(s):  
Transcription Factors ◽  
Complex Interplay ◽  
Myb Transcription Factors ◽  
Aliphatic Glucosinolates ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

2013 ◽  
Vol 25 (10) ◽  
pp. 4135-4149 ◽  
Author(s):  
Janine Höll ◽  
Alessandro Vannozzi ◽  
Stefan Czemmel ◽  
Claudio D'Onofrio ◽  
Amanda R. Walker ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Vitis Vinifera ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
Stilbene Biosynthesis ◽  
R2r3 Myb Transcription Factors

R2R3 MYB Transcription Factors – Functions outside the DNA-Binding Domain

2019 ◽  
Vol 24 (10) ◽  
pp. 934-946 ◽  
Author(s):  
Peter S. Millard ◽  
Birthe B. Kragelund ◽  
Meike Burow
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

Expression and Molecular Characterization of ZmMYB-IF35 and Related R2R3-MYB Transcription Factors

2007 ◽  
Vol 37 (2) ◽  
pp. 155-164 ◽  
Author(s):  
George F. Heine ◽  
Vinod Malik ◽  
Anusha P. Dias ◽  
Erich Grotewold
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors
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