The biochemical characterization of a high-stearic acid sunflower mutant reveals the coordinated regulation of stearoyl-acyl carrier protein desaturases

2008 ◽  
Vol 46 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Joaquín J. Salas ◽  
Loubna Youssar ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Stearic Acid ◽  
Biochemical Characterization ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
High Stearic Acid ◽  
Sunflower Mutant ◽  
Coordinated Regulation

Biochemical characterization of 3-ketoacyl-acyl carrier protein synthase II from leek epidermis

2000 ◽  
Vol 28 (6) ◽  
pp. 610-613 ◽  
Author(s):  
F. Domergue ◽  
D. Post-Beittenmiller
Keyword(s):  
Fatty Acid Synthase ◽  
Biochemical Characterization ◽  
Acyl Carrier Protein ◽  
Allium Porrum ◽  
Carrier Protein ◽  
Substrate Specificities ◽  
Absolute Requirement ◽  
Acyl Chains ◽  
Different Tissues

In order to define its possible involvement in production of stearic acid for wax biosynthesis, the presence of 3-ketoacyl acyl synthase II (KAS II) activity was investigated in different tissues of leek (Allium porrum L.) leaves. KAS II activity was identified in sheath and lamina epidermis, as well as in underlying parenchyma. In all three tissues, activity was inhibited by 50% on addition of l00μM cerulenin, and showed an absolute requirement for acyl-ACP substrates. More interestingly, the different tissues did not display similar KAS II substrate specificities. Parenchyma and lamina epidermis tissues presented typical KAS II activities, since C18 0-ACP was the exclusive product. In contrast, in sheath epidermis, KAS II activity resulted in the synthesis of acyl-chains up to 22 carbons in length, suggesting the existence in this tissue of an unusual KAS II. This activity was sufficient to elongate all of the palmitoyl-ACP produced by the fatty acid synthase, suggesting that C18 0 is the substrate of the microsomal elongases involved in wax biosynthesis.

scholarly journals Biochemical Characterization of Acyl Carrier Protein (AcpM) and Malonyl-CoA:AcpM Transacylase (mtFabD), Two Major Components ofMycobacterium tuberculosisFatty Acid Synthase II

2001 ◽  
Vol 276 (30) ◽  
pp. 27967-27974 ◽  
Author(s):  
Laurent Kremer ◽  
K. Madhavan Nampoothiri ◽  
Sarah Lesjean ◽  
Lynn G. Dover ◽  
Steven Graham ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Acyl Carrier Protein ◽  
Carrier Protein

Molecular and biochemical characterization of Toxoplasma gondii β-hydroxyacyl-acyl carrier protein dehydratase (FABZ)

2008 ◽  
Vol 102 (6) ◽  
pp. 1301-1309 ◽  
Author(s):  
George Dautu ◽  
Akio Ueno ◽  
Biscah Munyaka ◽  
Gabriella Carmen ◽  
Souichi Makino ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Biochemical Characterization ◽  
Acyl Carrier Protein ◽  
Carrier Protein

Molecular cloning and characterization of two β-ketoacyl-acyl carrier protein synthase I genes from Jatropha curcas L.

2017 ◽  
Vol 214 ◽  
pp. 152-160 ◽  
Author(s):  
Wangdan Xiong ◽  
Qian Wei ◽  
Pingzhi Wu ◽  
Sheng Zhang ◽  
Jun Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Jatropha Curcas ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Jatropha Curcas L

Genetic control of high stearic acid content in the seed oil of the sunflower mutant CAS-3

1999 ◽  
Vol 99 (3-4) ◽  
pp. 663-669 ◽  
Author(s):  
B. Pérez-Vich ◽  
R. Garcés ◽  
J. M. Fernández-Martínez
Keyword(s):  
Stearic Acid ◽  
Genetic Control ◽  
Acid Content ◽  
Seed Oil ◽  
Stearic Acid Content ◽  
High Stearic Acid ◽  
Sunflower Mutant
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