scholarly journals Dose-dependent induction of CPP or CPA by intra-pVTA ethanol: Role of mu opioid receptors and effects on NMDA receptors

Author(s):  
Yolanda Campos-Jurado ◽  
Lucía Martí-Prats ◽  
Jose A. Morón ◽  
Ana Polache ◽  
Luis Granero ◽  
...  
2020 ◽  
Author(s):  
Yolanda Campos-Jurado ◽  
Lucía Martí-Prats ◽  
Jose A. Morón ◽  
Ana Polache ◽  
Luis Granero ◽  
...  

AbstractThe neurobiological mechanisms underlying alcohol motivational properties are still not fully understood, however, the mu-opioid receptors (MORs) have been evidenced as central elements in the manifestation of the alcohol reinforcing properties. Drug-associated environmental stimuli can trigger alcohol relapse and promote alcohol consumption whereby N-methyl-D-aspartate (NMDA) receptors play a pivotal role. Here we sought to demonstrate, for the first time, that ethanol induces conditioned place preference or aversion (CPP or CPA) when administered locally into the ventral tegmental area (VTA) and the associated role of MORs. We further analyzed the changes in the expression and mRNA levels of GluN1 and GluN2A subunits in designated brain areas. The expression of CPP or CPA was characterized following intra-VTA ethanol administration and we showed that either reinforcing (CPP) or aversive (CPA) properties are dependent on the dose administered (ranging here from 35 to 300 nmol). Furthermore, the critical contribution of local MORs in the acquisition of CPP was revealed by a selective antagonist, namely β-Funaltrexamine. Finally, modifications of the expression of NMDA receptor subunits in the Nucleus Accumbens (NAc) and Hippocampus after ethanol-induced CPP were analyzed at the proteomic and transcriptomic levels by western blot and In Situ Hybridation RNAscope techniques, respectively. Results showed that the mRNA levels of GluN2A but not GluN1 in NAc are higher after ethanol CPP. These novel results pave the way for further characterisation of the mechanisms by which ethanol motivational properties are associated with learned environmental cues.


2006 ◽  
Vol 18 (6) ◽  
pp. 300-300
Author(s):  
T Money ◽  
B Dean ◽  
E Scarr

2012 ◽  
Vol 225 (1) ◽  
pp. 127-140 ◽  
Author(s):  
Katharine M. Seip-Cammack ◽  
Brian Reed ◽  
Yong Zhang ◽  
Ann Ho ◽  
Mary Jeanne Kreek

2020 ◽  
Vol 16 (10) ◽  
pp. 20200485 ◽  
Author(s):  
S. J. Charles ◽  
M. Farias ◽  
V. van Mulukom ◽  
A. Saraswati ◽  
S. Dein ◽  
...  

Religious rituals are universal human practices that play a seminal role in community bonding. In two experiments, we tested the role of mu-opioids as the active factor fostering social bonding. We used a mu-opioid blocker (naltrexone) in two double-blind studies of rituals from different religious traditions. We found the same effect across both studies, with naltrexone leading to significantly lower social bonding compared with placebo. These studies suggest that mu-opioids play a significant role in experiences of social bonding within ritual contexts.


2015 ◽  
Vol 50 (suppl 1) ◽  
pp. i29.4-i30
Author(s):  
L. Hipolito ◽  
M. J. Sanchez-Catalán ◽  
L. Martí-Prats ◽  
A. Orrico ◽  
M. J. Cano-Cebrián ◽  
...  

2020 ◽  
Author(s):  
Awinita Barpujari ◽  
Neil Ford ◽  
Shaoqiu He ◽  
Qian Huang ◽  
Claire Gaveriaux Ruff ◽  
...  

2021 ◽  
Vol 5 (1) ◽  
pp. s31
Author(s):  
Brian Kim ◽  
Thomas Sciascia ◽  
Gil Yosipovitch

Abstract not available.


Sign in / Sign up

Export Citation Format

Share Document