A new cell culture protocol for enrichment and genetic modification of adult canine Schwann cells suitable for peripheral nerve tissue engineering

2009 ◽  
Vol 87 (1) ◽  
pp. 140-142 ◽  
Author(s):  
K. Haastert ◽  
P. Seef ◽  
V.M. Stein ◽  
A. Tipold ◽  
C. Grothe
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Genetic Modification ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
Cell Culture Protocol ◽  
Culture Protocol

In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

2011 ◽  
Vol 175-176 ◽  
pp. 220-223 ◽  
Author(s):  
Ai Jun Hu ◽  
Bao Qi Zuo ◽  
Feng Zhang ◽  
Qing Lan ◽  
Huan Xiang Zhang
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Silk Fibroin ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
And Migration ◽  
Silk Fibroin Nanofibers

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

Peripheral Nerve Tissue Engineering: Autologous Schwann Cells vs. Transdifferentiated Mesenchymal Stem Cells

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730023
Author(s):  
Gerburg Keilhoff ◽  
Alexander Goihl ◽  
Felix Stang ◽  
Gerald Wolf ◽  
Hisham Fansa
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

Genetically modified canine Schwann cells—In vitro and in vivo evaluation of their suitability for peripheral nerve tissue engineering

2010 ◽  
Vol 186 (2) ◽  
pp. 202-208 ◽  
Author(s):  
Ruth Schmitte ◽  
Andrea Tipold ◽  
Veronika M. Stein ◽  
Henning Schenk ◽  
Cornelia Flieshardt ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Genetically Modified ◽  
Nerve Tissue ◽  
In Vivo Evaluation ◽  
Nerve Tissue Engineering

Peripheral Nerve Tissue Engineering: Autologous Schwann Cells vs. Transdifferentiated Mesenchymal Stem Cells

2006 ◽  
Vol 12 (6) ◽  
pp. 1451-1465 ◽  
Author(s):  
Gerburg Keilhoff ◽  
Alexander Goihl ◽  
Felix Stang ◽  
Gerald Wolf ◽  
Hisham Fansa
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

scholarly journals Cyclic tensile stimulation enrichment of Schwann cell-laden auxetic hydrogel scaffolds towards peripheral nerve tissue engineering

2020 ◽  
Vol 195 ◽  
pp. 108982 ◽  
Author(s):  
Yi-Wen Chen ◽  
Kan Wang ◽  
Chia-Che Ho ◽  
Chia-Tze Kao ◽  
Hooi Yee Ng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Hydrogel Scaffolds ◽  
Nerve Tissue Engineering

scholarly journals Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration

2015 ◽  
Vol 15 (6) ◽  
pp. 612-621 ◽  
Author(s):  
Lorena R. Lizarraga-Valderrama ◽  
Rinat Nigmatullin ◽  
Caroline Taylor ◽  
John W. Haycock ◽  
Frederik Claeyssens ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

scholarly journals Vascularization Strategies for Peripheral Nerve Tissue Engineering

2018 ◽  
Vol 301 (10) ◽  
pp. 1657-1667 ◽  
Author(s):  
Papon Muangsanit ◽  
Rebecca J. Shipley ◽  
James B. Phillips
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

Novel electrospun conduit based on polyurethane/collagen enhanced by nanobioglass for peripheral nerve tissue engineering

2022 ◽  
pp. 1-15
Author(s):  
Somayeh Tofighi Nasab ◽  
Nasim Hayati Roodbari ◽  
Vahabodin Goodarzi ◽  
Hossein Ali Khonakdar ◽  
Kourosh Mansoori ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering
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