scholarly journals Analytical performance comparison of four SARS-CoV-2 RT-qPCR primer-probe sets for wastewater samples

2022 ◽  
Vol 806 ◽  
pp. 150572
Author(s):  
Shuxin Zhang ◽  
Xuan Li ◽  
Jiahua Shi ◽  
Muttucumaru Sivakumar ◽  
Stephen Luby ◽  
...  
2016 ◽  
Vol 508 ◽  
pp. 19-24 ◽  
Author(s):  
K. Yugender Goud ◽  
Atul Sharma ◽  
Akhtar Hayat ◽  
Gaëlle Catanante ◽  
K. Vengatajalabathy Gobi ◽  
...  

2020 ◽  
Author(s):  
Sasima Chotithammakul ◽  
Dakrong Pissuwan

The sensitivity of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed, which affect the sensitivity and reproducibility of the assay testing procedure. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal amplification, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a biomarker for confronting chronic kidney disease.


Author(s):  
Molla Asmare ◽  
Mustafa Ilbas

Nowadays, the most decisive challenges we are fronting are perfectly clean energy making for equitable and sustainable modern energy access, and battling the emerging alteration of the climate. This is because, carbon-rich fuels are the fundamental supply of utilized energy for strengthening human society, and it will be sustained in the near future. In connection with this, electrochemical technologies are an emerging and domineering tool for efficiently transforming the existing scarce fossil fuels and renewable energy sources into electric power with a trivial environmental impact. Compared with conventional power generation technologies, SOFC that operate at high temperature is emerging as a frontrunner to convert the fuels chemical energy into electric power and permits the deployment of varieties of fuels with negligible ecological destructions. According to this critical review, direct ammonia is obtained as a primary possible choice and price-effective green fuel for T-SOFCs. This is because T-SOFCs have higher volumetric power density, mechanically stable, and high thermal shocking resistance. Also, there is no sealing issue problem which is the chronic issues of the planar one. As a result, the toxicity of ammonia to use as a fuel is minimized if there may be a leakage during operation. It is portable and manageable that can be work everywhere when there is energy demand. Besides, manufacturing, onboard hydrogen deposition, and transportation infrastructure connected snags of hydrogen will be solved using ammonia. Ammonia is a low-priced carbon-neutral source of energy and has more stored volumetric energy compared with hydrogen. Yet, to utilize direct NH3 as a means of hydrogen carrier and an alternative green fuel in T-SOFCs practically determining the optimum operating temperatures, reactant flow rates, electrode porosities, pressure, the position of the anode, thickness and diameters of the tube are still requiring further improvement. Therefore, mathematical modeling ought to be developed to determine these parameters before planning for experimental work. Also, a performance comparison of AS, ES, and CS- T-SOFC powered with direct NH3 will be investigated and best-performed support will be carefully chosen for practical implementation and an experimental study will be conducted for verification based on optimum parameter values obtained from numerical modeling.


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