Hydrogen peroxide biosensor based on direct electron transfer of horseradish peroxidase with vapor deposited quantum dots

2009 ◽  
Vol 138 (1) ◽  
pp. 278-282 ◽  
Author(s):  
Zhan Wang ◽  
Qiao Xu ◽  
Hai-Qiao Wang ◽  
Qin Yang ◽  
Jiu-Hong Yu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Quantum Dots ◽  
Electron Transfer ◽  
Horseradish Peroxidase ◽  
Direct Electron Transfer ◽  
Hydrogen Peroxide Biosensor

scholarly journals Development of Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on Screen-Printed Carbon Electrode Modified With Silver Nanoparticles

2019 ◽  
pp. 2332-2340
Author(s):  
Ali Saad Elewi ◽  
Shatha Abdul Wadood ◽  
Abdul Kareem Mohammed Ali
Keyword(s):  
Hydrogen Peroxide ◽  
Silver Nanoparticles ◽  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Phosphate Buffer Solution ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Hydrogen Peroxide Biosensor ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

     The direct electron transfer behavior of hemoglobin that is immobilized onto screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and chitosan (CS) was studied in this work. Cyclic voltametry and spectrophotometry were used to characterize the hemoglobin (Hb) bioconjunction with AgNPs and CS. Results of the modified electrode showed quasi-reversible redox peaks with a formal potential of (-0.245V) versus Ag/AgCl in 0.1M phosphate buffer solution (PBS), pH7, at a scan rate of 0.1Vs-1. The charge transfer coefficient (α) was 0.48 and the apparent electron transfer rate constant (Ks) was 0.47s-1. The electrode was used as a hydrogen peroxide biosensor with a linear response over 3 to 240 µM and a detection limit of 0.6 µM. As a result, the modified biosensor here has exhibited a high sensitivity, good reproducibility and stability.

Sign in / Sign up

Export Citation Format

Share Document