Competitive electrochemical immunosensor for amyloid-beta 1-42 detection based on gold nanostructurated Screen-Printed Carbon Electrodes

2014 ◽  
Vol 201 ◽  
pp. 567-571 ◽  
Author(s):  
Estefanía Costa Rama ◽  
María Begoña González-García ◽  
Agustín Costa-García
2012 ◽  
Vol 31 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Marta M.P.S. Neves ◽  
María Begoña González-García ◽  
Henri P.A. Nouws ◽  
Agustín Costa-García

Molekul ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 1 ◽  
Author(s):  
Yeni Wahyuni Hartati ◽  
Muhammad Hilman Daniswara ◽  
Ratna Nurmalasari ◽  
Shabarni Gaffar ◽  
Toto Subroto

B-type natriuretic peptide (BNP) is a 32-amino acid polypeptide, a cardiac neuro hormone that specifically secreted from heart ventricle as a response towards the increase of volume and pressure in the heart. The determination of BNP concentration in patients blood is one of the method used to diagnose heart failure. An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes for the detection of the BNP antigen was developed in this study. Monoclonal anti-BNP capture antibody was immobilized on streptavidin-modified SPCEs to give a well oriented of antibody. Furthermore, a biotinylated anti-BNP that conjugated with horseradish peroxidase (HRP) was used as secondary antibody. The electrochemical signal produced by redox activity of substrate 3,3,5,5'-tetramethybezidine dihydrochloride (TMB/H2O2) was measured by differential pulse voltammetry. The BNP immunosensor showed a linear response between 1.0×10-2 and 1.0×102 ng/mL, and the limit of detection was 3.3 ng/mL. BNP immunosensor is a promising technology for the rapid and convenient detection of heart failure.


2010 ◽  
Vol 26 (2) ◽  
pp. 517-522 ◽  
Author(s):  
Marcos Eguílaz ◽  
María Moreno-Guzmán ◽  
Susana Campuzano ◽  
Araceli González-Cortés ◽  
Paloma Yáñez-Sedeño ◽  
...  

2019 ◽  
Vol 298 ◽  
pp. 126878 ◽  
Author(s):  
Rebeca Jiménez-Pérez ◽  
José González-Rodríguez ◽  
María-Isabel González-Sánchez ◽  
Beatriz Gómez-Monedero ◽  
Edelmira Valero

2017 ◽  
Vol 123 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Rui M. Ramos ◽  
Pedro F. Brandão ◽  
Luís M. Gonçalves ◽  
Vlastimil Vyskočil ◽  
José A. Rodrigues

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