Carbon stable isotope fractionation and trophic transfer of fatty acids in fungal based soil food chains

ABSTRACT Carbon stable isotope fractionation of tetrachloroethene (PCE) during reductive dechlorination by whole cells and crude extracts of Sulfurospirillum multivorans and Desulfitobacterium sp. strain PCE-S and the abiotic reaction with cyanocobalamin (vitamin B12) was studied. Fractionation was largest during the reaction with cyanocobalamin with αC = 1.0132. Stable isotope fractionation was lower but still in a similar order of magnitude for Desulfitobacterium sp. PCE-S (αC = 1.0052 to 1.0098). The isotope fractionation of PCE during dehalogenation by S. multivorans was lower by 1 order of magnitude (αC = 1.00042 to 1.0017). Additionally, an increase in isotope fractionation was observed with a decrease in cell integrity for both strains. For Desulfitobacterium sp. strain PCE-S, the carbon stable isotope fractionation factors were 1.0052 and 1.0089 for growing cells and crude extracts, respectively. For S. multivorans, αC values were 1.00042, 1.00097, and 1.0017 for growing cells, crude extracts, and the purified PCE reductive dehalogenase, respectively. For the field application of stable isotope fractionation, care is needed as fractionation may vary by more than an order of magnitude depending on the bacteria present, responsible for degradation.

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Carbon Stable Isotope Fractionation of Sulfamethoxazole during Biodegradation byMicrobacteriumsp. Strain BR1 and upon Direct Photolysis

Environmental Science & Technology ◽

10.1021/acs.est.5b00367 ◽

2015 ◽

Vol 49 (10) ◽

pp. 6029-6036 ◽

Cited By ~ 19

Author(s):

Jan Birkigt ◽

Tetyana Gilevska ◽

Benjamin Ricken ◽

Hans-Hermann Richnow ◽

Davide Vione ◽

...

Keyword(s):

Stable Isotope ◽

Isotope Fractionation ◽

Carbon Stable Isotope ◽

Direct Photolysis ◽

Stable Isotope Fractionation

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Enantioselective Carbon Stable Isotope Fractionation of Hexachlorocyclohexane during Aerobic Biodegradation bySphingobiumspp.

Environmental Science & Technology ◽

10.1021/es402197s ◽

2013 ◽

Vol 47 (20) ◽

pp. 11432-11439 ◽

Cited By ~ 45

Author(s):

Safdar Bashir ◽

Anko Fischer ◽

Ivonne Nijenhuis ◽

Hans-Hermann Richnow

Keyword(s):

Stable Isotope ◽

Isotope Fractionation ◽

Aerobic Biodegradation ◽

Carbon Stable Isotope ◽

Stable Isotope Fractionation

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Comparison of 1,2-dichloroethane, dichloroethene and vinyl chloride carbon stable isotope fractionation during dechlorination by two Dehalococcoides strains

Water Research ◽

10.1016/j.watres.2013.12.042 ◽

2014 ◽

Vol 52 ◽

pp. 146-154 ◽

Cited By ~ 25

Author(s):

Marie Schmidt ◽

Sascha Lege ◽

Ivonne Nijenhuis

Keyword(s):

Stable Isotope ◽

Vinyl Chloride ◽

Isotope Fractionation ◽

Carbon Stable Isotope ◽

Stable Isotope Fractionation

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Stable isotope fractionation of fatty acids of Daphnia fed laboratory cultures of microalgae

Limnologica ◽

10.1016/j.limno.2015.12.001 ◽

2016 ◽

Vol 56 ◽

pp. 23-29 ◽

Cited By ~ 11

Author(s):

Michail I. Gladyshev ◽

Olesia N. Makhutova ◽

Elena S. Kravchuk ◽

Olesia V. Anishchenko ◽

Nadezhda N. Sushchik

Keyword(s):

Fatty Acids ◽

Stable Isotope ◽

Isotope Fractionation ◽

Stable Isotope Fractionation ◽

Laboratory Cultures

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Incorporation of Mg and Sr and oxygen and carbon stable isotope fractionation in cultured Ammonia tepida

Marine Micropaleontology ◽

10.1016/j.marmicro.2012.04.006 ◽

2012 ◽

Vol 92-93 ◽

pp. 16-28 ◽

Cited By ~ 20

Author(s):

Paula Diz ◽

Christine Barras ◽

Emmanuelle Geslin ◽

Gert-Jan Reichart ◽

Edouard Metzger ◽

...

Keyword(s):

Stable Isotope ◽

Isotope Fractionation ◽

Carbon Stable Isotope ◽

Stable Isotope Fractionation

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Carbon stable isotope fractionation in marine systems: open ocean studies and laboratory studies

Stable Isotopes ◽

10.1201/9781003076865-15 ◽

2020 ◽

pp. 239-256

Author(s):

A.M. Johnston ◽

H. Kennedy

Keyword(s):

Stable Isotope ◽

Isotope Fractionation ◽

Open Ocean ◽

Carbon Stable Isotope ◽

Laboratory Studies ◽

Marine Systems ◽

Stable Isotope Fractionation ◽

Ocean Studies

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Unravelling the process of petroleum hydrocarbon biodegradation in different filter materials of constructed wetlands by stable isotope fractionation and labelling studies

Biodegradation ◽

10.1007/s10532-021-09942-1 ◽

2021 ◽

Author(s):

Andrea Watzinger ◽

Melanie Hager ◽

Thomas Reichenauer ◽

Gerhard Soja ◽

Paul Kinner

Keyword(s):

Stable Isotope ◽

Constructed Wetlands ◽

Hydrogen Isotope ◽

Petroleum Hydrocarbon ◽

Isotope Fractionation ◽

Isotope Effects ◽

Filter Material ◽

Hydrocarbon Biodegradation ◽

Stable Isotope Fractionation ◽

Filter Materials

AbstractMaintaining and supporting complete biodegradation during remediation of petroleum hydrocarbon contaminated groundwater in constructed wetlands is vital for the final destruction and removal of contaminants. We aimed to compare and gain insight into biodegradation and explore possible limitations in different filter materials (sand, sand amended with biochar, expanded clay). These filters were collected from constructed wetlands after two years of operation and batch experiments were conducted using two stable isotope techniques; (i) carbon isotope labelling of hexadecane and (ii) hydrogen isotope fractionation of decane. Both hydrocarbon compounds hexadecane and decane were biodegraded. The mineralization rate of hexadecane was higher in the sandy filter material (3.6 µg CO2 g−1 day−1) than in the expanded clay (1.0 µg CO2 g−1 day−1). The microbial community of the constructed wetland microcosms was dominated by Gram negative bacteria and fungi and was specific for the different filter materials while hexadecane was primarily anabolized by bacteria. Adsorption / desorption of petroleum hydrocarbons in expanded clay was observed, which might not hinder but delay biodegradation. Very few cases of hydrogen isotope fractionation were recorded in expanded clay and sand & biochar filters during decane biodegradation. In sand filters, decane was biodegraded more slowly and hydrogen isotope fractionation was visible. Still, the range of observed apparent kinetic hydrogen isotope effects (AKIEH = 1.072–1.500) and apparent decane biodegradation rates (k = − 0.017 to − 0.067 day−1) of the sand filter were low. To conclude, low biodegradation rates, small hydrogen isotope fractionation, zero order mineralization kinetics and lack of microbial biomass growth indicated that mass transfer controlled biodegradation.

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