Three-dimensional culture of rabbit nucleus pulposus cells in collagen microspheres

2011 ◽  
Vol 11 (10) ◽  
pp. 947-960 ◽  
Author(s):  
Minting Yuan ◽  
Kam W. Leong ◽  
Barbara P. Chan
Keyword(s):  
Nucleus Pulposus ◽  
Three Dimensional ◽  
Nucleus Pulposus Cells ◽  
Three Dimensional Culture

scholarly journals Variations in Gene and Protein Expression in Canine Chondrodystrophic Nucleus Pulposus Cells following Long-Term Three-Dimensional Culture

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63120 ◽  
Author(s):  
Munetaka Iwata ◽  
Hiroki Ochi ◽  
Yoshinori Asou ◽  
Hirotaka Haro ◽  
Takeshi Aikawa ◽  
...  
Keyword(s):  
Protein Expression ◽  
Nucleus Pulposus ◽  
Three Dimensional ◽  
Nucleus Pulposus Cells ◽  
Gene And Protein Expression ◽  
Three Dimensional Culture

scholarly journals Development of Three-Dimensional Culture System for Maintenance of Bovine Nucleus Pulposus Cells

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376659-s-0034-1376659
Author(s):  
H. Y. Li ◽  
J. N. Ma ◽  
W. H. Chooi ◽  
D. Chan ◽  
K. S. E. Cheah ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Culture System ◽  
Three Dimensional ◽  
Nucleus Pulposus Cells ◽  
Three Dimensional Culture

scholarly journals SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yunyun Zhuo ◽  
Haoming Wang ◽  
Luetao Zou ◽  
Yiyang Wang ◽  
Yanzhu Hu ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Nucleus Pulposus Cell ◽  
Nucleus Pulposus Cells ◽  
High Magnitude ◽  
Culture Model ◽  
Cellular Apoptosis ◽  
Rat Tail

Mechanical overloading-induced nucleus pulposus cell (NPC) apoptosis plays a core role in the pathogenesis of intervertebral disc degeneration. In this study, we investigated the involvement of mammalian silent information regulator 2 homolog (SIRT1) in NPC apoptosis under high-magnitude compression. Our results showed that high-magnitude compression aggravated cellular apoptosis and attenuated the expression levels of SIRT1 and microtubule-associated protein-1 light chain-3B (LC3B) in rat NPCs in a three-dimensional (3D) cell culture model and an in vivo rat tail compression model, whereas SIRT1 overexpression in NPCs partially reversed these indicators. Moreover, SIRT1 overexpression increased the formation of the LC3B/Fas complex, alleviated activation of the NF-κB pathway, and reduced NPC apoptosis. Finally, downregulation of LC3B partially activated the NF-κB pathway and aggravated NPC apoptosis. Overall, upregulation of SIRT1 increases formation of the LC3B/Fas complex, which contributes to suppression of NPC apoptosis by inhibiting the NF-κB pathway under high compressive stress.

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