The metabolism of cholesterol and certain hormonal steroids by Treponema denticola

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.

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Hormonal Steroids: Proceedings of the Third International Congress on Hormonal Steroids, Hamburg, 7‐12 September, 1970

The Medical Journal of Australia ◽

10.5694/j.1326-5377.1973.tb200015.x ◽

1973 ◽

Vol 1 (5) ◽

pp. 258-258

Keyword(s):

International Congress ◽

The Third ◽

Hormonal Steroids

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Cleavage of Human Immunoglobulin G by Treponema denticola

Anaerobe ◽

10.1006/anae.2000.0361 ◽

2001 ◽

Vol 7 (1) ◽

pp. 1-4 ◽

Cited By ~ 5

Author(s):

Daniel Grenier ◽

Denis Mayrand

Keyword(s):

Immunoglobulin G ◽

Human Immunoglobulin ◽

Treponema Denticola ◽

Human Immunoglobulin G

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Analytical Methods for the Determination of Neuroactive Steroids

Biomolecules ◽

10.3390/biom11040553 ◽

2021 ◽

Vol 11 (4) ◽

pp. 553

Author(s):

Michal Kaleta ◽

Jana Oklestkova ◽

Ondřej Novák ◽

Miroslav Strnad

Keyword(s):

Nervous System ◽

Steroid Hormone ◽

Neuroactive Steroids ◽

Physiological Changes ◽

Biological Matrices ◽

Pathological Conditions ◽

Reliable Monitoring ◽

Hormonal Steroids ◽

Endocrine Tissues

Neuroactive steroids are a family of all steroid-based compounds, of both natural and synthetic origin, which can affect the nervous system functions. Their biosynthesis occurs directly in the nervous system (so-called neurosteroids) or in peripheral endocrine tissues (hormonal steroids). Steroid hormone levels may fluctuate due to physiological changes during life and various pathological conditions affecting individuals. A deeper understanding of neuroactive steroids’ production, in addition to reliable monitoring of their levels in various biological matrices, may be useful in the prevention, diagnosis, monitoring, and treatment of some neurodegenerative and psychiatric diseases. The aim of this review is to highlight the most relevant methods currently available for analysis of neuroactive steroids, with an emphasis on immunoanalytical methods and gas, or liquid chromatography combined with mass spectrometry.

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