Background:
With the outbreak of high throughput next-generation sequencing (NGS), the biological
research of drug discovery has been directed towards the oncology and infectious disease therapeutic areas, with
extensive use in biopharmaceutical development and vaccine production.
Method:
In this review, an effort was made to address the basic background of NGS technologies, potential applications
of NGS in drug designing. Our purpose is also to provide a brief introduction of various Nextgeneration
sequencing techniques.
Discussions:
The high-throughput methods execute Large-scale Unbiased Sequencing (LUS) which comprises of
Massively Parallel Sequencing (MPS) or NGS technologies. The Next geneinvolved necessarily executes Largescale
Unbiased Sequencing (LUS) which comprises of MPS or NGS technologies. These are related terms that
describe a DNA sequencing technology which has revolutionized genomic research. Using NGS, an entire human
genome can be sequenced within a single day.
Conclusion:
Analysis of NGS data unravels important clues in the quest for the treatment of various lifethreatening
diseases and other related scientific problems related to human welfare.
Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation
