Low doses of ochratoxin A upregulate the protein expression of organic anion transporters Oat1, Oat2, Oat3 and Oat5 in rat kidney cortex

2009 ◽  
Vol 239 (3) ◽  
pp. 284-296 ◽  
Author(s):  
Vilim Žlender ◽  
Davorka Breljak ◽  
Marija Ljubojević ◽  
Dubravka Flajs ◽  
Daniela Balen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ochratoxin A ◽  
Rat Kidney ◽  
Organic Anion ◽  
Kidney Cortex ◽  
Low Doses ◽  
Organic Anion Transporters ◽  
Rat Kidney Cortex ◽  
Anion Transporters

Expression of renal organic anion transporters OAT1 and OAT3 in ochratoxin A-treated rats

2006 ◽  
Vol 164 ◽  
pp. S81 ◽  
Author(s):  
Vilim Žlender ◽  
Marija Ljubojević ◽  
Daniela Balen ◽  
Davorka Breljak ◽  
Naohiko Anzai ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Organic Anion ◽  
Organic Anion Transporters ◽  
Anion Transporters

Developmental expression of renal organic anion transporters in rat kidney and its effect on renal secretion of phenolsulfonphthalein

2012 ◽  
Vol 302 (12) ◽  
pp. F1640-F1649 ◽  
Author(s):  
Maki Nomura ◽  
Hideyuki Motohashi ◽  
Hiroko Sekine ◽  
Toshiya Katsura ◽  
Ken-ichi Inui
Keyword(s):  
Protein Expression ◽  
Organic Anion ◽  
Immunohistochemical Analysis ◽  
Tubular Secretion ◽  
Organic Anion Transporters ◽  
Adult Rats ◽  
Anion Transporters ◽  
Anionic Drugs ◽  
Clearance Study

Organic anion transporters (OAT1 and OAT3) and multidrug resistance-associated proteins (MRP2 and MRP4) play important roles in anionic drug secretion in renal proximal tubules. Changes in the expression of such transporters are considered to affect the tubular secretion of anionic drugs. The purpose of this study was to elucidate the developmental changes in the expression of OAT1, OAT3, MRP2, and MRP4 and their effects on the tubular secretion of drugs. The mRNA level of each transporter was measured by real-time PCR, and the protein expression was evaluated by Western blotting and immunohistochemical analysis. In addition, the tubular secretion of phenolsulfonphthalein (PSP) in infant (postnatal day 14) and adult rats was estimated based on in vivo clearance study. The protein expression of organic anion transporters were very low at postnatal day 0 and gradually increased with age. In postnatal day 14 rats, the expression of OAT1 and OAT3 seemed to be at almost mature levels, while MRP2 and MRP4 seemed to be at immature levels. Immunohistochemical analysis in the kidney of postnatal day 0 rats revealed OATs on the basolateral membrane and MRPs on the brush-border membrane. At postnatal day 0, the distribution of these transporters was restricted to the inner cortical region, while after postnatal day 14, it was identical to that in adult kidney. An in vivo clearance study revealed that the tubular secretion of PSP was significantly lower in postnatal day 14 rats than adult rats. These results indicate that age-dependent changes in organic anion transporter expression affect the tubular secretion of anionic drugs in pediatric patients.

scholarly journals Downregulation of organic anion transporters in rat kidney under ischemia/reperfusion-induced qacute renal failure

2007 ◽  
Vol 71 (6) ◽  
pp. 539-547 ◽  
Author(s):  
T. Matsuzaki ◽  
H. Watanabe ◽  
K. Yoshitome ◽  
T. Morisaki ◽  
A. Hamada ◽  
...  
Keyword(s):  
Renal Failure ◽  
Rat Kidney ◽  
Ischemia Reperfusion ◽  
Organic Anion ◽  
Organic Anion Transporters ◽  
Anion Transporters

scholarly journals Optimal Methods of Antigen Retrieval for Organic Anion Transporters in Cryosections of the Rat Kidney

2009 ◽  
Vol 60 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Hrvoje Brzica ◽  
Davorka Breljak ◽  
Marija Ljubojević ◽  
Daniela Balen ◽  
Vedran Micek ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Organic Anion ◽  
Staining Intensity ◽  
Antigen Retrieval ◽  
Organic Anion Transporters ◽  
Optimal Methods ◽  
Anion Transporters ◽  
Pre Treatment

Optimal Methods of Antigen Retrieval for Organic Anion Transporters in Cryosections of the Rat KidneyTo localise antigens by immunocytochemistry (IC), the samples of tissues or cells are usually denatured by fixation, and either frozen and cryosectioned, or embedded in paraffin before sectioning. p-Formaldehyde (PFA; formalin) is a common fixative, which preserves antigenicity of proteins, but damages the tissue/cell morphology and "masks" the antibody binding sites (epitopes). In order to "unmask" epitopes, some kind of antigen retrieval (AR) is used. The aim of this study was: a) to find an optimal AR method in cryosections of in vivo PFA-fixed kidneys for organic anion transporters (Oat) that reside in the basolateral (Oat1, Oat3) and brush-border membrane (Oat2, Oat5) of the rat renal proximal tubules, and b) using optimal method, to compare IC staining of Oats in kidneys that had been PFA-fixed in vivo or in vitro. IC staining in untreated cryosections was compared with that following detergent treatment or microwave heating in citrate buffer of pH 3, pH 6, or pH 8, with or without alcohol pre-treatment. The preferred AR method for Oat1, Oat2, and Oat5 was heating of cryosections at pH 6, and for Oat3 heating at pH 3, without alcohol pre-treatment. Compared with tissue fixed in vivo, tissue fixed in vitro exhibited damaged tubule morphology, similar staining intensity of Oat1 and Oat3, and higher staining intensity of Oat2 and Oat5. We conclude that for optimal IC presentation, each Oat in the rat kidney has to be treated individually, with different fixation and AR approach.

Ontogeny of Expression of Organic Anion Transporters 1 and 3 in Ovine Fetal and Neonatal Kidney

2005 ◽  
Vol 230 (9) ◽  
pp. 668-673 ◽  
Author(s):  
Charles E. Wood ◽  
Roderick Cousins ◽  
Daying Zhang ◽  
Maureen Keller-Wood
Keyword(s):  
Protein Expression ◽  
Renal Cortex ◽  
Organic Anion ◽  
Renal Medulla ◽  
Renal Tissue ◽  
Late Gestation ◽  
Organic Anion Transporters ◽  
Fetal Sheep ◽  
Anion Transporters ◽  
Ovine Fetal

Organic ions are excreted into the urine via the action of organic anion transporters (OATs). In adult kidney, both OAT1 and OAT3, both multispecific transporters, are abundant; OAT1 is a known transporter of para-aminohippurate (PAH) and OAT3 is a known transporter of sulfoconjugated estrogens. The present study was designed to test the hypotheses that the expression of both OAT1 and OAT3 are developmentally regulated and that the expression increases in late gestation. Fetal kidneys were collected at sacrifice of fetal sheep at 80, 100, 120, 130, and 145 days of gestation, as well as 1 day and 1 week after birth (n = 4–5 per group). Renal tissue was separated into cortex and medulla and snap-frozen in liquid nitrogen for later extraction of mRNA. The expression levels of OAT1 and OAT3 were measured using real-time reverse transcriptase polymerase chain reaction (RT-PCR), with specific probes and primers designed in our laboratory. Cellular distribution of protein expression was identified using immunohistochemistry with commercially available antisera. The OAT1 and OAT3 mRNA in renal cortex was increased in the more mature animals. At 145 days of gestation, OAT1 mRNA abundance was increased and remained elevated postnatally. Compared with prenatal ages, OAT3 mRNA was increased postnatally. The expression of both transporters was not significantly changed as a function of development in the renal medulla. The protein expression of OAT1 and OAT3 was identified in tubular epithelium in renal cortex, although the immunoreactivity for OAT1 was greater than for OAT3. We conclude that there is a developmental pattern of expression of both OAT1 and OAT3 in ovine renal cortex, and that the pattern of expression suggests that the function of both transporters is likely to be greater starting in late gestation.

Characterization of ochratoxin A transport by human organic anion transporters

Life Sciences ◽  
2001 ◽  
Vol 69 (18) ◽  
pp. 2123-2135 ◽  
Author(s):  
Kyu Yong Jung ◽  
Michio Takeda ◽  
Do Kyung Kim ◽  
Akihiro Tojo ◽  
Shinichi Narikawa ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Organic Anion ◽  
Organic Anion Transporters ◽  
Anion Transporters
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