scholarly journals Correction to "Downregulation of organic anion transporters in rat kidney under ischemia/reperfusion-induced qacute renal failure"

2007 ◽  
Vol 71 (10) ◽  
pp. 1079
Keyword(s):  
Renal Failure ◽  
Rat Kidney ◽  
Ischemia Reperfusion ◽  
Organic Anion ◽  
Organic Anion Transporters ◽  
Anion Transporters

scholarly journals Downregulation of organic anion transporters in rat kidney under ischemia/reperfusion-induced qacute renal failure

2007 ◽  
Vol 71 (6) ◽  
pp. 539-547 ◽  
Author(s):  
T. Matsuzaki ◽  
H. Watanabe ◽  
K. Yoshitome ◽  
T. Morisaki ◽  
A. Hamada ◽  
...  
Keyword(s):  
Renal Failure ◽  
Rat Kidney ◽  
Ischemia Reperfusion ◽  
Organic Anion ◽  
Organic Anion Transporters ◽  
Anion Transporters

Downregulation of organic anion transporters OAT1 and OAT3 correlates with impaired secretion of para-aminohippurate after ischemic acute renal failure in rats

2007 ◽  
Vol 292 (5) ◽  
pp. F1599-F1605 ◽  
Author(s):  
R. Schneider ◽  
C. Sauvant ◽  
B. Betz ◽  
M. Otremba ◽  
D. Fischer ◽  
...  
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Organic Anion ◽  
Organic Anions ◽  
Organic Anion Transporters ◽  
Protein Levels ◽  
Anion Transporters ◽  
Pah Clearance ◽  
Ischemic Acute Renal Failure

Ischemic acute renal failure (iARF) was described to reduce renal extraction of the organic anion para-aminohippurate (PAH) in humans. The rate-limiting step of renal organic anion secretion is its basolateral uptake into proximal tubular cells. This process is mediated by the organic anion transporters OAT1 and OAT3, which both have a broad spectrum of substrates including a variety of pharmaceutics and toxins. Using a rat model of iARF, we investigated whether impairing the secretion of the organic anion PAH might be associated with downregulation of OAT1 or OAT3. Inulin and PAH clearance was determined starting from 6 up to 336 h after ischemia-reperfusion (I/R) injury. Net secretion of PAH was calculated and OAT1 as well as OAT3 expression was analyzed by RT-PCR and Western blotting. Inulin and PAH clearance along with PAH net secretion were initially diminished after I/R injury with a gradual recovery during follow-up. This initial impairment after iARF was accompanied by decreased mRNA and protein levels of OAT1 and OAT3 in clamped animals compared with sham-operated controls. In correlation to the improvement of kidney function, both mRNA and protein levels of OAT1 and OAT3 were upregulated during the follow-up. Thus decreased expression of OAT1 and OAT3 is sufficient to explain the decline of PAH secretion after iARF. As a result, this may have substantial impact on excretion kinetics and half-life of organic anions. As a consequence, the biological effects of a variety of organic anions may be affected after iARF.

scholarly journals Optimal Methods of Antigen Retrieval for Organic Anion Transporters in Cryosections of the Rat Kidney

2009 ◽  
Vol 60 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Hrvoje Brzica ◽  
Davorka Breljak ◽  
Marija Ljubojević ◽  
Daniela Balen ◽  
Vedran Micek ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Organic Anion ◽  
Staining Intensity ◽  
Antigen Retrieval ◽  
Organic Anion Transporters ◽  
Optimal Methods ◽  
Anion Transporters ◽  
Pre Treatment

Optimal Methods of Antigen Retrieval for Organic Anion Transporters in Cryosections of the Rat KidneyTo localise antigens by immunocytochemistry (IC), the samples of tissues or cells are usually denatured by fixation, and either frozen and cryosectioned, or embedded in paraffin before sectioning. p-Formaldehyde (PFA; formalin) is a common fixative, which preserves antigenicity of proteins, but damages the tissue/cell morphology and "masks" the antibody binding sites (epitopes). In order to "unmask" epitopes, some kind of antigen retrieval (AR) is used. The aim of this study was: a) to find an optimal AR method in cryosections of in vivo PFA-fixed kidneys for organic anion transporters (Oat) that reside in the basolateral (Oat1, Oat3) and brush-border membrane (Oat2, Oat5) of the rat renal proximal tubules, and b) using optimal method, to compare IC staining of Oats in kidneys that had been PFA-fixed in vivo or in vitro. IC staining in untreated cryosections was compared with that following detergent treatment or microwave heating in citrate buffer of pH 3, pH 6, or pH 8, with or without alcohol pre-treatment. The preferred AR method for Oat1, Oat2, and Oat5 was heating of cryosections at pH 6, and for Oat3 heating at pH 3, without alcohol pre-treatment. Compared with tissue fixed in vivo, tissue fixed in vitro exhibited damaged tubule morphology, similar staining intensity of Oat1 and Oat3, and higher staining intensity of Oat2 and Oat5. We conclude that for optimal IC presentation, each Oat in the rat kidney has to be treated individually, with different fixation and AR approach.

scholarly journals Arsenic and Mercury Containing Traditional Chinese Medicine (Realgar and Cinnabar) Strongly Inhibit Organic Anion Transporters, Oat1 and Oat3,In Vivoin Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Wen-Hao Yu ◽  
Na Zhang ◽  
Jin-Feng Qi ◽  
Chen Sun ◽  
Yong-Hui Wang ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Renal Failure ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Organic Anion ◽  
Pharmacokinetic Parameters ◽  
Organic Anion Transporters ◽  
Toxic Heavy Metals ◽  
Anion Transporters

Toxic heavy metals, including mercury (Hg) and arsenic (As), accumulate preferentially in kidneys and always cause acute renal failure. The aim of this study was to investigate whether these samples affect organic anion transporters, Oat1 and Oat3,in vivoin mice kidney. Mice (n=10) were orally treated with investigational samples. After last administration, all mice were i.v.p-aminohippuric acid (PAH), and the blood and kidneys samples were collected. The concentrations of PAH were quantified by spectrophotometry. mRNA expressions of Oat1 and Oat3 were assayed by real-time PCR. In comparison with corresponding control, major pharmacokinetic parameters of PAH in sera were significantly changed by investigational samples (p<0.05), PAH accumulations in the kidney tissues were significantly higher (p<0.05), PAH uptake by renal slices was greatly reduced, Oat1 and Oat3 mRNA expression were significantly inhibited in investigational sample groups. Arsenic and mercury containing traditional Chinese medicine (Realgar and Cinnabar) probably induce kidney damage through inhibiting several members of the organic anion transporters (such as OAT1 and OAT3).

Low doses of ochratoxin A upregulate the protein expression of organic anion transporters Oat1, Oat2, Oat3 and Oat5 in rat kidney cortex

2009 ◽  
Vol 239 (3) ◽  
pp. 284-296 ◽  
Author(s):  
Vilim Žlender ◽  
Davorka Breljak ◽  
Marija Ljubojević ◽  
Dubravka Flajs ◽  
Daniela Balen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ochratoxin A ◽  
Rat Kidney ◽  
Organic Anion ◽  
Kidney Cortex ◽  
Low Doses ◽  
Organic Anion Transporters ◽  
Rat Kidney Cortex ◽  
Anion Transporters
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