Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) method for the measurement of 2,4-dichlorophenoxyacetic acid in human urine

An enzyme-linked immunosorbent assay (ELISA) method was developed to detect apple mosaic virus (ApMV) in Betulaalleghaniensis Britton. Tests for virus using bark and bud tissues of dormant trees were successful. ApMV was also detectable in old leaf tissue in August and September, as well as in newly emerging leaf tissue forced in a greenhouse in March. Whole crude antiserum used to coat ELISA plates in tests with bud tissues was a reliable substitute for purified immunoglobulin without loss of sensitivity or specificity. An attempt was made to use ELISA for quantifying virus concentrations in field samples of ApMV-infected birch leaves.

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Development of a Class-Selective Enzyme-Linked Immunosorbent Assay for Mercapturic Acids in Human Urine

Journal of Agricultural and Food Chemistry ◽

10.1021/jf000621b ◽

2000 ◽

Vol 48 (12) ◽

pp. 5913-5923 ◽

Cited By ~ 7

Author(s):

Christian Lohse ◽

Lynn L. Jaeger ◽

Norbert Staimer ◽

Jim R. Sanborn ◽

A. Daniel Jones ◽

...

Keyword(s):

Immunosorbent Assay ◽

Human Urine ◽

Enzyme Linked Immunosorbent Assay ◽

Mercapturic Acids

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Development of an Enzyme-Linked Immunosorbent Assay for Serotyping Ureaplasma urealyticum Strains Using Monoclonal Antibodies

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.8.1.52-57.2001 ◽

2001 ◽

Vol 8 (1) ◽

pp. 52-57 ◽

Cited By ~ 11

Author(s):

Fedoua Echahidi ◽

Gaëtan Muyldermans ◽

Sabine Lauwers ◽

Anne Naessens

Keyword(s):

Monoclonal Antibodies ◽

Immunosorbent Assay ◽

Ureaplasma Urealyticum ◽

Polyclonal Antibodies ◽

Enzyme Linked Immunosorbent Assay ◽

Elisa Method ◽

Cross Reactions ◽

Antigenic Preparation ◽

Negative Controls ◽

Reference Strains

ABSTRACT Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to enable the serotyping of U. urealyticum isolates from primary broth cultures. Each of the 14 serotype reference strains was tested against 14 selected MAbs. Homologous reactions were very strong, while heterologous reactions were negligible. Three cross-reactions were observed: MAb 5 cross-reacted with serotype 2, MAb 14 cross-reacted with serotype 3, and MAb 8 cross-reacted with serotype 13. Despite the cross-reactions observed, all the serotype reference strains ofU. urealyticum could be identified and differentiated using a combination of MAbs. Reproducibility was analyzed with a fractionated antigenic preparation and with several freshly prepared antigens of the same strain. No significant interrun variation was found with the fractionated antigen, but significant variations in optical density (OD) values were found when freshly prepared antigens were tested. However, the variation in OD values did not influence the overall interpretation of the ELISA: reactions with homologous MAbs were always prominent compared to those of the negative controls. This newly developed ELISA using MAbs seems promising for serotyping of U. urealyticum clinical isolates.

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SOME RESULTS OF RESEARCH WORK ON PREVENTION OF ECHINOCOCCOSIS IN KYRGYZSTAN

10.31016/978-5-9902341-5-4.2020.21.428-431 ◽

2020 ◽

pp. 428-431

Author(s):

Tursunov ◽

Isaev ◽

Ibragimova

Keyword(s):

Veterinary Medicine ◽

Immunosorbent Assay ◽

Research Work ◽

Preventive Treatment ◽

Enzyme Linked Immunosorbent Assay ◽

Positive Outcomes ◽

Kyrgyz Republic ◽

Laboratory Studies ◽

Elisa Method ◽

Almost All

The Kyrgyz Research Institute of Veterinary Medicine is a co-executor of the Strategy for the Echinococcosis Control (2013–2018) and monitored dogs epizootological examinations after preventive treatment with azinox. Laboratory studies of biomaterials (feces) for infection with helminths were carried out along with the traditional coprological method, a more sensitive enzyme-linked immunosorbent assay (ELISA). The results of laboratory studies of fecal samples from dogs showed that the activities of the "Strategy for the Control of Echinococcosis in the Kyrgyz Republic", including the treatment of dogs with azinox, gave positive outcomes. From 2014 to 2018 2260 feces samples from deworming dogs from almost all regions of Kyrgyzstan were examined. At the same time, the infection of dogs with taeniidae is steadily decreasing; it has also been noted that the main joints of parasitocenosis in dogs are taeniidae, toxocaras, mesocestoids, and eimeria. In the studies conducted on the infection of dogs with taeniidae, the coprological and more sensitive ELISA method was used. Using both helminthological studies, a decrease in the invasion of dogs by taeniidae was confirmed. Thus, in the period from 2014 (the beginning of the Strategy) to 2018, the average invasion of dogs was reduced from 20.2 to 14.3%.

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Diarrhetic Shellfish Poisoning: Evaluation of Enzyme-Linked Immunosorbent Assay Methods for Determination of Dinophyslstoxin-2

Journal of AOAC International ◽

10.1093/jaoac/78.6.1403 ◽

1995 ◽

Vol 78 (6) ◽

pp. 1403-1407 ◽

Cited By ~ 7

Author(s):

Eoin P Carmody ◽

Kevin J James ◽

Seán S Kelly

Keyword(s):

Immunosorbent Assay ◽

Cross Reactivity ◽

Regulatory Control ◽

Enzyme Linked Immunosorbent Assay ◽

Shellfish Poisoning ◽

Diarrhetic Shellfish Poisoning ◽

Elisa Method ◽

Multiple Samples ◽

Liquid Chromatographic

Abstract Dinophysistoxin-2 (DTX-2), an isomer of okadaic acid (OA), recently has been found in Irish waters. DTX-2 was the predominant toxin during prolonged infestations in cultivated mussels along the southwest coast of Ireland. Substantial variations in toxin levels may exist both horizontally and vertically in the water column. The need to take multiple samples and the ethical concern about the use of mammals for routine quality control of shellfish prompted examination of 2 commercially available enzyme-linked immunosorbent assay (ELISA) methods, designed to detect OA, for determination of both OA and DTX-2. One ELISA method (DSPCheck, Sceti Co. Ltd., (Tokyo, Japan) showed good cross-reactivity (40 ± 5%) with standard DTX-2. This study showed that both ELISA methods show good correlation with the liquid chromatographic analysis of 9-anthryldiazomethane derivatives when OA is the predominant toxin present. The sensitivity was also good for OA determination using both methods, which allowed toxin measurement at 10 ng/mL (0.5 ng/well). This level is equivalent to 0.03 μg/g mussel meat. Blank mussel samples spiked with DTX-2 standards gave a good linear correlation (r = 0.997) with this ELISA method when toxin levels were 0.03-0.3 μg/g mussel meat. This range is appropriate for regulatory control of diarrhetic shellfish poisoning.

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