A label-free assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on poly(thymine)-templated copper nanoparticles

A novel label-free biosensor for highly sensitive sensing of the activity as well as inhibition of T4 PNKP based on poly T-templated fluorescent CuNPs and nicking enzyme-assisted signal amplification.

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Label-free colorimetric assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on G-quadruplex/hemin DNAzyme

Analytical Biochemistry ◽

10.1016/j.ab.2016.10.022 ◽

2017 ◽

Vol 517 ◽

pp. 18-21 ◽

Cited By ~ 16

Author(s):

Haisheng Liu ◽

Changbei Ma ◽

Jun Wang ◽

Hanchun Chen ◽

Kemin Wang

Keyword(s):

Phosphatase Activity ◽

Colorimetric Assay ◽

Label Free ◽

Polynucleotide Kinase ◽

G Quadruplex ◽

T4 Polynucleotide Kinase

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Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

Analytical Methods ◽

10.1039/c9ay02283j ◽

2020 ◽

Vol 12 (6) ◽

pp. 807-812

Author(s):

Xu Wu ◽

Shuyi He ◽

Julia Xiaojun Zhao

Keyword(s):

Kinase Activity ◽

Sybr Green ◽

Fluorescence Assay ◽

Sybr Green I ◽

Label Free ◽

Cleavage Reaction ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy

Analytical Methods ◽

10.1039/c5ay03335g ◽

2016 ◽

Vol 8 (9) ◽

pp. 1989-1994 ◽

Cited By ~ 6

Author(s):

Changbei Ma ◽

Shunxin Jin ◽

Jun Wang ◽

Kemin Wang ◽

Haisheng Liu ◽

...

Keyword(s):

Electron Transfer ◽

Phosphatase Activity ◽

Photoinduced Electron Transfer ◽

Fluorescence Method ◽

Terminal Transferase ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

A novel fluorescence method for detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy.

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Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Chemical Communications ◽

10.1039/c3cc47444e ◽

2014 ◽

Vol 50 (40) ◽

pp. 5313-5315 ◽

Cited By ~ 108

Author(s):

Hong-Zhang He ◽

Ka-Ho Leung ◽

Wei Wang ◽

Daniel Shiu-Hin Chan ◽

Chung-Hang Leung ◽

...

Keyword(s):

Kinase Activity ◽

Label Free ◽

Polynucleotide Kinase ◽

G Quadruplex ◽

T4 Polynucleotide Kinase

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Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors

Molecular and Cellular Probes ◽

10.1016/j.mcp.2015.11.002 ◽

2015 ◽

Vol 29 (6) ◽

pp. 500-502 ◽

Cited By ~ 8

Author(s):

Changbei Ma ◽

Shunxin Jin ◽

Haisheng Liu ◽

Kun Xia ◽

Jianhua Tang ◽

...

Keyword(s):

Fluorescence Probe ◽

Thioflavin T ◽

Label Free ◽

Label Free Detection ◽

Polynucleotide Kinase ◽

T4 Polynucleotide Kinase

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Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

The Analyst ◽

10.1039/c5an01032b ◽

2015 ◽

Vol 140 (16) ◽

pp. 5450-5453 ◽

Cited By ~ 11

Author(s):

Lu Zhou ◽

Xiaoqiang Shen ◽

Na Sun ◽

Kewei Wang ◽

Yuanyuan Zhang ◽

...

Keyword(s):

Kinase Activity ◽

Detection Method ◽

Label Free ◽

Strand Displacement ◽

Polynucleotide Kinase ◽

G Quadruplex ◽

Fluorescence Light ◽

T4 Polynucleotide Kinase

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

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