New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1955-1959 ◽  
Author(s):  
Huinan Chen ◽  
Zihan Wang ◽  
Xu Chen ◽  
Kai Lou ◽  
Anzhi Sheng ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
New Method ◽  
Sensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Reaction ◽  
T4 Polynucleotide Kinase

A new method has been developed for the sensitive detection of T4 PNKP activity based on the isothermal EXPonential amplification reaction.

A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy

2016 ◽  
Vol 8 (9) ◽  
pp. 1989-1994 ◽  
Author(s):  
Changbei Ma ◽  
Shunxin Jin ◽  
Jun Wang ◽  
Kemin Wang ◽  
Haisheng Liu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Phosphatase Activity ◽  
Photoinduced Electron Transfer ◽  
Fluorescence Method ◽  
Terminal Transferase ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel fluorescence method for detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy.

A WS2 nanosheet based sensing platform for highly sensitive detection of T4 polynucleotide kinase and its inhibitors

Nanoscale ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 6866-6872 ◽  
Author(s):  
Jia Ge ◽  
Li-Juan Tang ◽  
Qiang Xi ◽  
Xi-Ping Li ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Highly Sensitive Detection ◽  
T4 Polynucleotide Kinase

A nanobiosensor was developed based on a WS2 nanosheet for detection of T4 polynucleotide kinase with low interference and superior sensitivity.

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

2015 ◽  
Vol 51 (10) ◽  
pp. 1815-1818 ◽  
Author(s):  
Chunxia Song ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Qing Wang ◽  
Jianbo Liu ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Sensitive Detection ◽  
Activity Assay ◽  
Enhanced Fluorescence ◽  
Cyclodextrin Polymer ◽  
Polynucleotide Kinase ◽  
Kinase Activity Assay ◽  
T4 Polynucleotide Kinase

A strategy for performing a polynucleotide kinase activity assay has been developed based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction.

A novel fluorescence method for the highly sensitive detection of T4 polynucleotide kinase based on polydopamine nanotubes

2019 ◽  
Vol 43 (42) ◽  
pp. 16753-16758 ◽  
Author(s):  
Xue-Ping Shen ◽  
Jia Ge ◽  
Juan Chen ◽  
Yan-Mei Shen ◽  
Hong-Min Meng ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Highly Sensitive Detection ◽  
T4 Polynucleotide Kinase

A novel fluorescence method has been developed for the detection of T4 PNK using FRET between dye-labeled ssDNA and PDANTs.

3'-Phosphatase activity in T4 polynucleotide kinase

Biochemistry ◽  
1977 ◽  
Vol 16 (23) ◽  
pp. 5120-5126 ◽  
Author(s):  
Vicki Cameron ◽  
Olke C. Uhlenbeck
Keyword(s):  
Phosphatase Activity ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Development of a highly sensitive sensing platform for T4 polynucleotide kinase phosphatase and its inhibitors based on WS2 nanosheets

2014 ◽  
Vol 6 (18) ◽  
pp. 7212-7217 ◽  
Author(s):  
Xiaomeng Liu ◽  
Jia Ge ◽  
Xiangyu Wang ◽  
Zhan Wu ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
Activity Assay ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Novel Strategy ◽  
Ws2 Nanosheets ◽  
Nucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel strategy for nucleotide kinase phosphatase activity assay based on WS2 nanosheets.

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