Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors

2015 ◽  
Vol 29 (6) ◽  
pp. 500-502 ◽  
Author(s):  
Changbei Ma ◽  
Shunxin Jin ◽  
Haisheng Liu ◽  
Kun Xia ◽  
Jianhua Tang ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Thioflavin T ◽  
Label Free ◽  
Label Free Detection ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Calf thymus DNA-stabilized polythiophene fluorescence probe for label-free detection of spermine

The Analyst ◽  
2012 ◽  
Vol 137 (23) ◽  
pp. 5565 ◽  
Author(s):  
Jian Wang ◽  
Qing Zhang ◽  
Zhong De Liu ◽  
Cheng Zhi Huang
Keyword(s):  
Fluorescence Probe ◽  
Calf Thymus Dna ◽  
Label Free ◽  
Calf Thymus ◽  
Label Free Detection

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

2014 ◽  
Vol 50 (40) ◽  
pp. 5313-5315 ◽  
Author(s):  
Hong-Zhang He ◽  
Ka-Ho Leung ◽  
Wei Wang ◽  
Daniel Shiu-Hin Chan ◽  
Chung-Hang Leung ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Label Free ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
T4 Polynucleotide Kinase

A label-free fluorescence method for the detection of uracil DNA glycosylase activity based on G-quadruplex formation

2016 ◽  
Vol 8 (41) ◽  
pp. 7453-7459 ◽  
Author(s):  
Changbei Ma ◽  
Kefeng Wu ◽  
Jun Wang ◽  
Hailun He ◽  
Feng Ning ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Thioflavin T ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Quadruplex Formation

A simple fluorescence based biosensor for label-free detection of uracil DNA glycosylase activity based on G-quadruplex formation using a thioflavin T probe is reported.

Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5450-5453 ◽  
Author(s):  
Lu Zhou ◽  
Xiaoqiang Shen ◽  
Na Sun ◽  
Kewei Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Detection Method ◽  
Label Free ◽  
Strand Displacement ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Fluorescence Light ◽  
T4 Polynucleotide Kinase

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

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