3'-Phosphatase activity in T4 polynucleotide kinase

Biochemistry ◽  
1977 ◽  
Vol 16 (23) ◽  
pp. 5120-5126 ◽  
Author(s):  
Vicki Cameron ◽  
Olke C. Uhlenbeck
Keyword(s):  
Phosphatase Activity ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy

2016 ◽  
Vol 8 (9) ◽  
pp. 1989-1994 ◽  
Author(s):  
Changbei Ma ◽  
Shunxin Jin ◽  
Jun Wang ◽  
Kemin Wang ◽  
Haisheng Liu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Phosphatase Activity ◽  
Photoinduced Electron Transfer ◽  
Fluorescence Method ◽  
Terminal Transferase ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel fluorescence method for detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy.

New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1955-1959 ◽  
Author(s):  
Huinan Chen ◽  
Zihan Wang ◽  
Xu Chen ◽  
Kai Lou ◽  
Anzhi Sheng ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
New Method ◽  
Sensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Reaction ◽  
T4 Polynucleotide Kinase

A new method has been developed for the sensitive detection of T4 PNKP activity based on the isothermal EXPonential amplification reaction.

Development of a highly sensitive sensing platform for T4 polynucleotide kinase phosphatase and its inhibitors based on WS2 nanosheets

2014 ◽  
Vol 6 (18) ◽  
pp. 7212-7217 ◽  
Author(s):  
Xiaomeng Liu ◽  
Jia Ge ◽  
Xiangyu Wang ◽  
Zhan Wu ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
Activity Assay ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Polynucleotide Kinase ◽  
Novel Strategy ◽  
Ws2 Nanosheets ◽  
Nucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel strategy for nucleotide kinase phosphatase activity assay based on WS2 nanosheets.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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