Bacillamide, a novel algicide from the marine bacterium, Bacillus sp. SY-1, against the harmful dinoflagellate, Cochlodinium polykrikoides

Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production.

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The optimal of xylanase production conditions using empty fruit bunch raw biomass by marine bacterium Bacillus sp LBF-001

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v11i3.211 ◽

2016 ◽

Vol 11 (3) ◽

Author(s):

Hans Wijaya ◽

Ahmad Thontowi ◽

Nanik Rahmani ◽

Yopi Yopi

Keyword(s):

16S Rdna ◽

Marine Bacterium ◽

Xylanase Activity ◽

Empty Fruit Bunch ◽

Bacillus Sp ◽

Marine Microorganisms ◽

16S Rdna Gene ◽

Xylanase Production ◽

Rdna Gene ◽

Bacterium Bacillus

Several xylanases have been studied recently, but few xylanases are from marine microorganisms have been reported. Marine bacterium Bacillus sp. LBF-001 was isolated from Pari Island Kepulauan Seribu in Indonesia. The purposes of this study are to identify of 16S rDNA gene from marine bacterium LBF-001 and to optimize medium conditions including kind and concentration of biomass, nitrogen source, pH and temperature. With 16S rDNA gene analysis that LBF-001 isolate have 99% identity with Bacillus pumilus HT-Z4-B2 (KJ526885). Fermentation for producing xylanases was done by using several agricultural residues under solid-state fermentation (SSF). The optimum condition for xylanase production by isolate Bacillus sp. LBF-001 are 2.5% empty fruit bunch, 0.6% lactose broth, pH values was 6.5, temperature condition was 30oC, under submerged fermentation with shaking at 150 rpm for 48 hours fermentation and giving the xylanase activity 10.85 U/mL.

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Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp. ALSHL3

Biologia ◽

10.2478/s11756-009-0190-8 ◽

2009 ◽

Vol 64 (6) ◽

Cited By ~ 12

Author(s):

Keni Vidilaseris ◽

Karina Hidayat ◽

Debbie Retnoningrum ◽

Zeily Nurachman ◽

Achmad Noer ◽

...

Keyword(s):

Marine Bacterium ◽

Biochemical Characterization ◽

Scanning Electron Micrographs ◽

Bacillus Sp ◽

Starch Granules ◽

Raw Starch ◽

Optimum Ph ◽

Bacterium Bacillus ◽

Rdna Analysis

AbstractAn Indonesian marine bacterial isolate, which belongs to genus of Bacillus sp. based on 16S rDNA analysis and was identified as Bacillus filicolonicus according to its morphology and physiology, produced a raw starch degrading α-amylase. The partially purified α-amylase using a maize starch affinity method exhibited an optimum pH and temperature of 6.0 and 60°C, respectively. The enzyme retained 72% of its activity in the presence of 1.5 M NaCl. Scanning electron micrographs showed that the α-amylase was capable of degrading starch granules of rice and maize. This α-amylase from Bacillus sp. ALSHL3 was classified as a saccharifying enzyme since its major final degradation product was glucose, maltose, and maltotriose.

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