scholarly journals The optimal of xylanase production conditions using empty fruit bunch raw biomass by marine bacterium Bacillus sp LBF-001

2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Hans Wijaya ◽  
Ahmad Thontowi ◽  
Nanik Rahmani ◽  
Yopi Yopi
Keyword(s):  
16S Rdna ◽  
Marine Bacterium ◽  
Xylanase Activity ◽  
Empty Fruit Bunch ◽  
Bacillus Sp ◽  
Marine Microorganisms ◽  
16S Rdna Gene ◽  
Xylanase Production ◽  
Rdna Gene ◽  
Bacterium Bacillus

Several xylanases have been studied recently, but few xylanases are from marine microorganisms have been reported. Marine bacterium Bacillus sp. LBF-001 was isolated from Pari Island Kepulauan Seribu in Indonesia. The purposes of this study are to identify of 16S rDNA gene from marine bacterium LBF-001 and to optimize medium conditions including kind and concentration of biomass, nitrogen source, pH and temperature. With 16S rDNA gene analysis that LBF-001 isolate have 99% identity with Bacillus pumilus HT-Z4-B2  (KJ526885). Fermentation for producing xylanases was done by using several agricultural residues under solid-state fermentation (SSF). The optimum condition for xylanase production by isolate Bacillus sp. LBF-001 are 2.5% empty fruit bunch, 0.6% lactose broth, pH values was 6.5, temperature condition was 30oC, under submerged fermentation with shaking at 150 rpm for 48 hours fermentation and giving the xylanase activity 10.85 U/mL.

scholarly journals Optimization of alkali-thermostable and cellulase-free eylanase production from Bacillus Sp.

2012 ◽  
Vol 19 ◽  
pp. 7-14
Author(s):  
SCD Sharma ◽  
MS Shovon ◽  
AKM Asaduzzaman ◽  
MG Sarowar Jahan ◽  
T Yeasmin ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Agar Medium ◽  
Xylanase Activity ◽  
Effect Of Temperature ◽  
Bacillus Sp ◽  
Dilution Technique ◽  
Isolation And Identification ◽  
Xylanase Production ◽  
Optimization Of Process Parameters ◽  
Bacterium Bacillus

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (4000 U/L) by a Bacillus sp. was attained when the medium containing 0.5% wheat bran xylan and peptone at pH 8.0 and 50-55°C within 48-60 h. The partially purified xylanase was optimally active at pH 9.0 and 55°C. The xylanase showed high substrate activity towards wheat bran xylan but no activity towards cellulose, carboxymethyl cellulose and starch. Thus the enzyme was alkali-thermostable and cellulase free xylanase. Conclusion: The results obtained in this study suggest that the Bacillus sp. used is highly potential and useful for the production of cellulase free xylanase. DOI: http://dx.doi.org/10.3329/jbs.v19i0.12994 J. bio-sci. 19: 7-14, 2011

scholarly journals Isolation and Molecular Identification of Biosurfactant Producing Soil Bacteria

2019 ◽  
Vol 7 (4.14) ◽  
pp. 77
Author(s):  
N A N Zamani ◽  
T E Tengku Zainal Mulok ◽  
R Mat Nor ◽  
. .
Keyword(s):  
16S Rdna ◽  
Soil Bacteria ◽  
Bacterial Species ◽  
Gene Sequencing ◽  
Bacillus Sp ◽  
16S Rdna Gene ◽  
Gram Positive ◽  
Rdna Gene ◽  
Emulsification Index ◽  
16S Rdna Gene Sequencing

Biosurfactants are amphiphilic compound, having hydrophilic and hydrophobic moieties enabling them to reduce surface and interfacial tension at the surface. Their unique properties are applied in various industries such as foaming and wetting agents, emulsifiers, detergents and bioremediation. A total of 98 isolates showed biosurfactant activity using hemolytic activity, drop collapse test and oil spreading assay. All isolates were rod-shaped, Gram positive and majority of them were non-endospore former. Only the isolates showing the highest percentage of emulsification index (E24) and ability to reduce tension were used for species identification using 16S rDNA gene sequencing which were isolates A1(6) and A2(1). Both isolates were identified as Bacillus sp. cp-h50 and Bacillus sp. XT-24 respectively, rod-shaped, endospore former and Gram positive. The biosurfactant produced by both species showed high emulsification index (E24) (A1(6), 63.3% and A2(1), 46.7%) and good surfactant capacity. The size of amplified gene of 16S rDNA gene was approximately 1.5 kb. These features provide evidence that both species could be a potential biosurfactant producer with proper optimization for the production of biosurfactant. The biosurfactant produced by both bacterial species were identified as surfactin using Fourier Transform Infrared Spectroscopy (FTIR).  

Biodegradation of 2,2' 4,4-tetrabromodiphenyl Ether in an Aerobic Environment by a Novel Strain of Bacillus sp.

2013 ◽  
Vol 771 ◽  
pp. 45-49
Author(s):  
Jian Fei Guan ◽  
Ji Hua Wang ◽  
Xiang Liu ◽  
Jia Xin ◽  
Shan Shan Zhang ◽  
...  
Keyword(s):  
16S Rdna ◽  
Sequence Similarity ◽  
Electronic Waste ◽  
Aerobic Condition ◽  
Optimal Growth ◽  
Waste Recycling ◽  
Bacillus Sp ◽  
16S Rdna Gene ◽  
Growth Environment ◽  
Rdna Gene

The environmental safety of 2,2,4,4-tetrabromodiphenyl ether (BDE-47), a widely used flame retardant, has been the topic of controversial discussions during the past decade years. In this study, we investigated the BDE-47 degradation by a novel bacteria obtained from electronic waste recycling site soil sample in Taizhou, China. Using biochemical characteristics and 16S rDNA gene sequencing, the strain was closely related to Bacillus sp. with a 99% 16S rDNA gene sequence similarity. It could use BDE-47 as the sole carbon source and energy source for its growth. The optimal growth environment was in the presence of BDE-47 at concentrations to 50-200 ug/L, pH 6.0 to 8.0 and 37°C when agitated at 160 rpm. For the initial concentrations of 100 ug/L, 50% of the BDE-47 was degraded. This is the first report of BDE47-degrading strain from the genus of Bacillus sp. under aerobic condition.

scholarly journals Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

Marine Drugs ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 86 ◽  
Author(s):  
Peng Chen ◽  
Yueming Zhu ◽  
Yan Men ◽  
Yan Zeng ◽  
Yuanxia Sun
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Bacterium Bacillus

scholarly journals Effects of Physico-Chemical Parameters on Actinomycetes Communities during Composting of Agricultural Waste

2019 ◽  
Vol 11 (8) ◽  
pp. 2229
Author(s):  
Yuanping Li ◽  
Yanrong Chen ◽  
Yaoning Chen ◽  
Yanxin Wu ◽  
Chun Zhang ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Moisture Content ◽  
16S Rdna ◽  
Community Composition ◽  
Agricultural Waste ◽  
Chemical Parameters ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Gene Abundance ◽  
Physico Chemical

The objective of this study was to investigate the influence of physico-chemical parameters on Actinomycetes communities and to prioritize those parameters that contributed to Actinomycetes community composition during the composting of agricultural waste. Denaturing gradient gel electrophoresis of polymerase chain reaction (PCR-DGGE) and redundancy analysis (RDA) were used to determine the relationships between those parameters and Actinomycetes community composition. Quantitative PCR (qPCR) and regression analysis were used to monitor the 16S rDNA copy numbers of Actinomycetes and to analyse the correlations between physico-chemical parameters and Actinomyces 16S rDNA gene abundance, respectively. The RDA results showed that moisture content, water soluble carbon (WSC) and pH (p < 0.05) made the main contributions to the temporal variations of Actinomycetes community composition. The output of the regression analysis indicated that moisture content (R2 = 0.407, p < 0.01) showed a negative linear relationship with the Actinomyces 16S rDNA gene abundance.

scholarly journals Identificarion of contaminant bacteria in cachaça yeast by 16s rDNA gene sequencing

2008 ◽  
Vol 65 (5) ◽  
pp. 508-515 ◽  
Author(s):  
Osmar Vaz de Carvalho-Netto ◽  
Daniel Dias Rosa ◽  
Luis Eduardo Aranha Camargo
Keyword(s):  
16S Rdna ◽  
Fermentation Process ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Operational Taxonomic Units ◽  
Bacterial Contaminants ◽  
Three Samples ◽  
Two Samples ◽  
Microbiological Control ◽  
One Year

Cachaça is a typical Brazilian liquor produced from the distillation of fermented sugarcane juice mainly by Saccharomyces cerevisiae. Most of the domestic production is artisanal, and producers usually are not concerned regarding microbiological control of the fermentation. This study aimed to characterize the contaminant bacterial community of the yeast used in the production of cachaça in an artisanal still. Four samples were collected, of which one (NA) was used for comparison purposes and was collected one year earlier. The remaining samples were collected at three different periods: at the end of the first day of fermentation (NP), after fifteen days (NS), and thirty days after the same yeast was used (NT). Five hundred and eighty-seven sequences were analyzed from the partial sequencing of the 16S rDNA gene. Sequence analyses revealed the presence of 170 operational taxonomic units (OTUs). Of these, only one was shared among three samples and seventeen were shared between two samples. The remaining 152 OTUs were identified only once in distinct samples indicating that the contaminant bacterial population is highly dynamic along the fermentation process. Statistical analyses revealed differences in bacterial composition among samples. Undescribed species in the literature on yeasts of cachaça were found, such as Weissella cibaria, Leuconostoc citreum, and some species of Lactobacillus, in addition to some unknown bacteria. The community of bacteria in the fermentation process is much more complex than it was previously considered. No previous report is known regarding the use of this technique to determine bacterial contaminants in yeast for the production of cachaça.

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