Extracellular vesicle-associated tissue factor activity is increased in prostate cancer patients with disseminated intravascular coagulation and induced by cellular interactions in vitro

2018 ◽  
Vol 164 ◽  
pp. S230
Author(s):  
L. Hell ◽  
C. Ay ◽  
T. Däullary ◽  
L.M. Mauracher ◽  
E. Grilz ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Patients ◽  
Tissue Factor ◽  
Disseminated Intravascular Coagulation ◽  
Extracellular Vesicle ◽  
Cellular Interactions ◽  
Factor Activity ◽  
Intravascular Coagulation ◽  
Tissue Factor Activity

scholarly journals Extracellular Vesicle-Associated Tissue Factor Activity in Prostate Cancer Patients with Disseminated Intravascular Coagulation

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1487
Author(s):  
Lena Hell ◽  
Thomas Däullary ◽  
Vanessa Burghart ◽  
Lisa-Marie Mauracher ◽  
Ella Grilz ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Patients ◽  
Tissue Factor ◽  
Disseminated Intravascular Coagulation ◽  
Mononuclear Cells ◽  
Coagulation Cascade ◽  
Dependent Manner ◽  
Peripheral Blood Mononuclear ◽  
Intravascular Coagulation

Patients with advanced prostate cancer may develop fulminant disseminated intravascular coagulation (DIC). Circulating extracellular vesicles (EVs)-exposing tissue factor (TF), the initiator of the coagulation cascade, may play an important role. We included 7 prostate cancer patients with DIC, 10 age- and stage-matched cancer controls without DIC, and 10 age-matched healthy male individuals. EV-TF activity was highly elevated in prostate cancer patients with DIC (11.40 pg/mL; range: 4.34–27.06) compared with prostate cancer patients without DIC (0.09 pg/mL; range: 0.00–0.30, p = 0.001) and healthy controls (0.18 pg/mL; range: 0.09–0.54; p = 0.001). Only EVs from patients with DIC showed reduced fibrin clot formation time of pooled plasma in a TF-dependent manner. Next, we performed in vitro co-culture experiments including EVs derived from a prostate cancer cell line with high (DU145) and low (LNCaP) TF expression, peripheral blood mononuclear cells (PBMCs), and platelets. Co-incubation of DU145 EVs with PBMCs and platelets significantly increased EV-TF activity in conditioned medium and induced TF activity on monocytes. No such effects were seen in co-culture experiments with LNCaP EVs. In conclusion, the findings indicate that elevated EV-TF activity plays a role in the development of prostate-cancer-related DIC and may result from interactions between tumor-derived EVs, monocytes, and platelets.

scholarly journals Microparticle-associated tissue factor activity in dogs with disseminated intravascular coagulation

2020 ◽  
Vol 82 (1) ◽  
pp. 56-60
Author(s):  
Kosuke KOBAYASHI ◽  
Kenji BABA ◽  
Masaya IGASE ◽  
Takako Shimokawa MIYAMA ◽  
Satoshi KAMBAYASHI ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Disseminated Intravascular Coagulation ◽  
Factor Activity ◽  
Intravascular Coagulation ◽  
Tissue Factor Activity

Longitudinal analysis of extracellular vesicle-associated tissue factor activity in cancer patients

2020 ◽  
Vol 195 ◽  
pp. 215-218
Author(s):  
Eva-Maria Reitter ◽  
Alexandra Kaider ◽  
Gerald Prager ◽  
Cihan Ay ◽  
Ingrid Pabinger ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Tissue Factor ◽  
Longitudinal Analysis ◽  
Extracellular Vesicle ◽  
Factor Activity ◽  
Tissue Factor Activity

scholarly journals Increased microparticle tissue factor activity in cancer patients with Venous Thromboembolism

2010 ◽  
Vol 125 (6) ◽  
pp. 511-512 ◽  
Author(s):  
David A. Manly ◽  
Jianguo Wang ◽  
Sam L. Glover ◽  
Raj Kasthuri ◽  
Howard A. Liebman ◽  
...  
Keyword(s):  
Venous Thromboembolism ◽  
Cancer Patients ◽  
Tissue Factor ◽  
Factor Activity ◽  
Tissue Factor Activity
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