scholarly journals Development of Tissue Factor Activity in Mononuclear Cells Cultured in Vitro

1978 ◽  
Vol 125 (3) ◽  
pp. 213-222 ◽  
Author(s):  
TATSUHITO TONO-OKA
Keyword(s):  
Tissue Factor ◽  
Mononuclear Cells ◽  
Factor Activity ◽  
Tissue Factor Activity ◽  
Cells Cultured

Selective Increases of Procoagulant Activity in Rabbit Lymphoid Populations In Vitro Following Stimulation with Endotoxin: Dependence on Anatomic Source

1984 ◽  
Vol 51 (02) ◽  
pp. 228-231 ◽  
Author(s):  
Maria P McGee ◽  
Henry Rothberger ◽  
Tung-Kuang Lee
Keyword(s):  
Lymph Node ◽  
Tissue Factor ◽  
Mononuclear Cells ◽  
Procoagulant Activity ◽  
Mononuclear Leukocytes ◽  
Cell Populations ◽  
Factor Activity ◽  
Tissue Factor Activity

SummaryRabbit mononuclear leukocytes isolated from a variety of anatomic sites were examined for ability to generate procoagulant activity in vitro. Marrow, blood and spleen mononuclear cell populations were found to differ functionally from lymph node, thymus and alveolar populations by having much greater ability to increase in tissue factor activity in response to an endotoxin stimulus. Thus, after incubation in the presence of endotoxin, leukocytes obtained from marrow, blood, and spleen were found to increase in procoagulant activity characterized as tissue factor by 832, 1942 and 12.6 fold, respectively. In contrast, pulmonary alveolar macrophages increased in tissue factor activity only by 2.8 fold, and lymph node and thymus mononuclear cells showed little or no increases. These functional differences, demonstrated by exposing the six cell populations to endotoxin under controlled conditions in vitro, likely explain the similar pattern of anatomic selectivity of leukocyte tissue factor increases reported to occur in vivo during endotoxemia and Shwartzman reactions (1).

Effects of Anti-Neutrophil Cytoplasm Autoantibodies on Tissue Factor Activity by HL-60 Cells In Vitro

2003 ◽  
Vol 57 (1) ◽  
pp. 68-78 ◽  
Author(s):  
L. F. Flores-Suarez ◽  
R. Nowack ◽  
B. A. Yard ◽  
C.-E. Dempfle ◽  
F. J. Van Der Woude
Keyword(s):  
Tissue Factor ◽  
Factor Activity ◽  
Tissue Factor Activity

scholarly journals Macrophage factor X activator formation: metabolic requirements for synthesis of components

Blood ◽  
1985 ◽  
Vol 65 (1) ◽  
pp. 169-175 ◽  
Author(s):  
JW Jr Shands
Keyword(s):  
Tissue Factor ◽  
Actinomycin D ◽  
Factor Vii ◽  
Soluble Factor ◽  
Factor X ◽  
Factor Activity ◽  
In Vitro Production ◽  
Tissue Factor Activity ◽  
Culture Supernatants

Abstract The in vitro production of factor VII-like material and of tissue factor activity by murine thioglycollate exudate macrophages was measured by amidolytic assays. Tissue factor activity was inducible by endotoxin, and its induction was inhibited by 1 microgram/mL of actinomycin D, 10 micrograms/mL of cycloheximide, and 0.2 micrograms/mL of tunicamycin. Soluble factor VII-like material was secreted by macrophages into culture supernatants. The amount produced was not influenced by further activation of the cells by endotoxin, nor was its production inhibited significantly by 1 microgram/mL actinomycin D or 0.2 micrograms/mL tunicamycin. The production of the factor VII-like material was inhibited by 10 micrograms/mL of cycloheximide, and its appearance in culture supernatants was enhanced significantly by the addition of vitamin K1. When lysates of activated macrophages were suspended in ultracentrifuged culture supernatants, a particulate factor X activator was formed. Centrifugation at 100,000 g pelleted the factor X activator and left no factor VII-like material in the supernatant. The data indicate that thioglycollate-induced exudate macrophages make and excrete factor VII-like material, and this production is not modulated by further activation. However, activation of the macrophages induces tissue factor production. The factor X activator appears to result from the interaction and complexing of the soluble factor VII-like material and the membrane-bound tissue factor.

Pathways of Stimulation of Tissue Factor Activity Generation by Leukocytes

1979 ◽  
Author(s):  
J. Niemetz ◽  
T. Muhlfelder
Keyword(s):  
Tissue Factor ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Complement Component ◽  
Factor Activity ◽  
Tissue Factor Activity ◽  
Activity Generation ◽  
Antigen Antibody ◽  
Stimulation Of

Leukocytes nay generate a potent procoagulant thromboplastic (tissue factor) activity (TFa) if appropriately stimulated. Among the stimulants endowing leukocytes with TFa are endotoxins (lipopolysaccharides: LPS), antigen-antibody, complexes and a complement component: the chemotactic fragment of C5 (C5fr) vhieh is a particularly potent activator. As LPS and antigen-antibody complexes can activate complement with ensuing generation of C5fr, the question arose whether C5fr is the sole terminal pathway for activation of leukocyte TFa. Studies of the amount of activity generated with the LPS and C5fr showed that normal leukocytes stimulated with C5fr produce significantly more TFa than leukocytes stimulated with LPS. In contrast, leukocytes from patients with paroxysmal nocturnal hemoglobinuria which respond normally to LPS, generate considerably less TFa when stimulated with C5fr. Furthermore, the-TFa in normal leukocytes - appears sooner following LPS stimulation than following C5fr stimulation. These results suggest that at least in vitro C5fr need not be the sole external stimulant for the generation of TFa.

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