Characterization of a new cytochrome P450 enzyme, CYP2S1, in rats: Its regulation by aryl hydrocarbon receptor agonists

Toxicology ◽  
2010 ◽  
Vol 267 (1-3) ◽  
pp. 91-98 ◽  
Author(s):  
Subrata Deb ◽  
Stelvio M. Bandiera
Keyword(s):  
Cytochrome P450 ◽  
Aryl Hydrocarbon Receptor ◽  
Cytochrome P450 Enzyme ◽  
Receptor Agonists ◽  
Aryl Hydrocarbon ◽  
P450 Enzyme

scholarly journals Characterization of aryl hydrocarbon receptor agonists in sediments of Wenyu River, Beijing, China

2009 ◽  
Vol 43 (9) ◽  
pp. 2441-2448 ◽  
Author(s):  
Jianping Luo ◽  
Mei Ma ◽  
Jinmiao Zha ◽  
Zijian Wang
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Receptor Agonists ◽  
Aryl Hydrocarbon ◽  
Beijing China

Characterization of Quinoline Yellow Dyes As Transient Aryl Hydrocarbon Receptor Agonists

2020 ◽  
Vol 33 (3) ◽  
pp. 742-750
Author(s):  
Patrick Tarnow ◽  
Catrin Zordick ◽  
Alex Bottke ◽  
Berit Fischer ◽  
Friederike Kühne ◽  
...  
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Receptor Agonists ◽  
Aryl Hydrocarbon ◽  
Quinoline Yellow

scholarly journals Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer–micelle association

2006 ◽  
Vol 395 (3) ◽  
pp. 641-652 ◽  
Author(s):  
Richard K. Hughes ◽  
Eric J. Belfield ◽  
Mylrajan Muthusamay ◽  
Anuja Khan ◽  
Arthur Rowe ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Cytochrome P450 ◽  
Medicago Truncatula ◽  
Water Soluble ◽  
Hydroperoxide Lyase ◽  
Cytochrome P450 Enzyme ◽  
Cytochrome P450 Enzymes ◽  
P450 Enzyme ◽  
Detergent Micelles

We describe the detailed biochemical characterization of CYP74C3 (cytochrome P450 subfamily 74C3), a recombinant plant cytochrome P450 enzyme with HPL (hydroperoxide lyase) activity from Medicago truncatula (barrel medic). Steady-state kinetic parameters, substrate and product specificities, RZ (Reinheitszahl or purity index), molar absorption coefficient, haem content, and new ligands for an HPL are reported. We show on the basis of gel filtration, sedimentation velocity (sedimentation coefficient distribution) and sedimentation equilibrium (molecular mass) analyses that CYP74C3 has low enzyme activity as a detergent-free, water-soluble, monomer. The enzyme activity can be completely restored by re-activation with detergent micelles, but not detergent monomers. Corresponding changes in the spin state equilibrium, and probably co-ordination of the haem iron, are novel for cytochrome P450 enzymes and suggest that detergent micelles have a subtle effect on protein conformation, rather than substrate presentation, which is sufficient to improve substrate binding and catalytic-centre activity by an order of magnitude. The kcat/Km of up to 1.6×108 M−1·s−1 is among the highest recorded, which is remarkable for an enzyme whose reaction mechanism involves the scission of a C–C bond. We carried out both kinetic and biophysical studies to demonstrate that this effect is a result of the formation of a complex between a protein monomer and a single detergent micelle. Association with a detergent micelle rather than oligomeric state represents a new mechanism of activation for membrane-associated cytochrome P450 enzymes. Highly concentrated and monodispersed samples of detergent-free CYP74C3 protein may be well suited for the purposes of crystallization and structural resolution of the first plant cytochrome P450 enzyme.

scholarly journals Identification and characterization of CYP79D6v4, a cytochrome P450 enzyme producing aldoximes in black poplar (Populus nigra)

2013 ◽  
Vol 8 (12) ◽  
pp. e27640 ◽  
Author(s):  
Sandra Irmisch ◽  
Sybille B Unsicker ◽  
Jonathan Gershenzon ◽  
Tobias G Köllner
Keyword(s):  
Cytochrome P450 ◽  
Populus Nigra ◽  
Cytochrome P450 Enzyme ◽  
Black Poplar ◽  
P450 Enzyme ◽  
Identification And Characterization

scholarly journals Structural characterization of CYP144A1 – a cytochrome P450 enzyme expressed from alternative transcripts in Mycobacterium tuberculosis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jude Chenge ◽  
Madeline E. Kavanagh ◽  
Max D. Driscoll ◽  
Kirsty J. McLean ◽  
Douglas B. Young ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cytochrome P450 ◽  
Structural Characterization ◽  
Cytochrome P450 Enzyme ◽  
Alternative Transcripts ◽  
P450 Enzyme
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