Determination of UV-induced DNA damages to suppress protein expression using reporter gene assay-based single cell cotransfection imaging cytometry

2011 ◽  
Vol 204 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Yu Kyung Tak ◽  
Won Young Kim ◽  
Enyoung Han ◽  
Min Jung Kim ◽  
Jae Ah Kim ◽  
...  
Keyword(s):  
Protein Expression ◽  
Single Cell ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Dna Damages ◽  
Gene Assay ◽  
Imaging Cytometry

Validation and application of a reporter gene assay for the determination of estrogenic endocrine disruptor activity in milk

2014 ◽  
Vol 69 ◽  
pp. 260-266 ◽  
Author(s):  
E. Wielogorska ◽  
C.T. Elliott ◽  
M. Danaher ◽  
L. Connolly
Keyword(s):  
Reporter Gene ◽  
Endocrine Disruptor ◽  
Reporter Gene Assay ◽  
Gene Assay

Evaluation of a Reporter Gene Assay for Bioactivity Determination of Therapeutic Interferons through a Collaborative Study

2016 ◽  
Vol 12 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Chun-ming Rao ◽  
De-ning Pei ◽  
Lei Yu ◽  
Jun Gao ◽  
Michel Girard ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Collaborative Study ◽  
Reporter Gene Assay ◽  
Gene Assay

Development and validation of a reporter gene assay for bioactivity determination of Anti-CGRP monoclonal antibodies

2021 ◽  
pp. 114291
Author(s):  
Xiao Guo ◽  
Chuanfei Yu ◽  
Lan Wang ◽  
Feng Zhang ◽  
Kaiqin Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Development And Validation

Development and validation of a novel reporter gene assay for determination of recombinant human thrombopoietin

2021 ◽  
Vol 99 ◽  
pp. 107982
Author(s):  
Jie Yuan ◽  
Jia Li ◽  
Lihua Yang ◽  
Yunying Lv ◽  
Chao Wang ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Development And Validation ◽  
Recombinant Human Thrombopoietin

scholarly journals A simple and robust reporter gene assay for measuring the bioactivity of anti-RANKL therapeutic antibodies

RSC Advances ◽  
2019 ◽  
Vol 9 (69) ◽  
pp. 40196-40202
Author(s):  
Chuanfei Yu ◽  
Lan Wang ◽  
Yongbo Ni ◽  
Junzhi Wang
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Therapeutic Antibodies ◽  
Gene Assay

The mechanism sketch of the reporter gene assay for the bioactivity determination of anti-RANKL mAbs.

scholarly journals In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Alberto Celma ◽  
Geeta Mandava ◽  
Agneta Oskarsson ◽  
Juan Vicente Sancho ◽  
Lubertus Bijlsma ◽  
...  
Keyword(s):  
Water Quality ◽  
Reporter Gene ◽  
Biological Activities ◽  
Reporter Gene Assay ◽  
Water Bodies ◽  
Good Tool ◽  
Adverse Health Effects ◽  
Aryl Hydrocarbon ◽  
Gene Assay

Abstract Background Fresh water bodies represent less than 1% of overall amount of water on earth and ensuring their quality and sustainability is pivotal. Although several campaigns have been performed to monitor the occurrence of micropollutants by means of chemical analysis, this might not cover the whole set of chemicals present in the sample nor the potential toxic effects of mixtures of natural and anthropogenic chemicals. In this sense, by selecting relevant toxicity endpoints when performing in vitro bioanalysis, effect-based methodologies can be of help to perform a comprehensive assessment of water quality and reveal biological activities relevant to adverse health effects. However, no prior bioanalytical study was performed in wetland water samples from the Spanish Mediterranean coastline. Methods Eleven samples from relevant water bodies from the Spanish Mediterranean coastline were collected to monitor water quality on 8 toxicity endpoints. Aryl hydrocarbon receptor (AhR), androgenicity (AR+ and AR−), estrogenicity (ER+ and ER−), oxidative stress response (Nrf2) and vitamin D receptor (VDR+ and VDR−) reporter gene assays were evaluated. Results AhR was the reporter gene assay showing a more frequent response over the set of samples (activated by 9 out of 11 samples), with TCDD-eq in the range 7.7–22.2 pM. For AR, ER and VDR assays sporadic activations were observed. Moreover, no activity was observed on the Nrf2 reporter gene assay. Wastewater and street runaway streams from Valencia could be responsible for enhanced activities in one of the water inputs in the Natural Park ‘L’Albufera’. Conclusions Water quality of relevant wetlands from the Spanish Mediterranean coastline has been evaluated. The utilization of a panel of 5 different bioassays to cover for different toxicity endpoints has demonstrated to be a good tool to assess water quality.

Evaluation of estrogenic activities of pesticides using an in vitro reporter gene assay

2005 ◽  
Vol 15 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Mihoko Kojima ◽  
Kenji Fukunaga * ◽  
Mari Sasaki ◽  
Masafumi Nakamura ◽  
Motohiro Tsuji ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay

Development of a novel reporter gene assay to evaluate antibody-dependent cellular phagocytosis for anti-CD20 therapeutic antibodies

2021 ◽  
Vol 100 ◽  
pp. 108112
Author(s):  
Chunyu Liu ◽  
Chuanfei Yu ◽  
Yalan Yang ◽  
Jing Huang ◽  
Xiaojuan Yu ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Therapeutic Antibodies ◽  
Gene Assay ◽  
Anti Cd20

Dinucleotide tag‐based parallel reporter gene assay method enables efficient identification of regulatory mutations

2021 ◽  
pp. 2100341
Author(s):  
Naixia Ren ◽  
Bo Li ◽  
Qingqing Liu ◽  
Lele Yang ◽  
Xiaodan Liu ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Assay Method ◽  
Gene Assay ◽  
Regulatory Mutations
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