Clinical graft evolution of lymphocytes, polymorphonuclear cells, and antigen expression in tubular renal cells in the urine sediment of 20 renal allograft recipients

2003 ◽  
Vol 35 (7) ◽  
pp. 2500-2505 ◽  
Author(s):  
J Péfaur ◽  
R Triviño ◽  
C Navarrete ◽  
E Oberhauser ◽  
M Melys ◽  
...  
Keyword(s):  
Renal Allograft ◽  
Antigen Expression ◽  
Polymorphonuclear Cells ◽  
Renal Cells ◽  
Urine Sediment

scholarly journals Analysis of Urine Sediment for Cytology and Antigen Expression in Acute Renal Allograft Rejection

2012 ◽  
Vol 137 (5) ◽  
pp. 816-824 ◽  
Author(s):  
Priti Chatterjee ◽  
Sandeep R. Mathur ◽  
Amit K. Dinda ◽  
Sandeep Guleria ◽  
Sandeep Mahajan ◽  
...  
Keyword(s):  
Allograft Rejection ◽  
Renal Allograft ◽  
Antigen Expression ◽  
Urine Sediment ◽  
Renal Allograft Rejection ◽  
Acute Renal Allograft Rejection ◽  
Analysis Of Urine

scholarly journals Cytomegalovirus in urinary sediment in patients with acute kidney injury

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Sahra Pajenda ◽  
Sebastian Kapps ◽  
Daniela Gerges ◽  
Gregor Hoermann ◽  
Ludwig Wagner ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Renal Allograft ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Extraction ◽  
Kidney Injury ◽  
Solid Organ ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Urine Sediment ◽  
Urinary Sediment

Abstract Background Immunosuppression in solid organ transplantation is associated with frequent infections. Renal allograft recipients are susceptible to opportunistic infections and can acquire human cytomegalovirus (HCMV) infections even within the allograft. There, HCMV can be found in both the glomerulus and tubular cells, but is mostly restricted to specific and circumscribed sites. Therefore, not all organ infections are identifiable by immunohistology for HCMV proteins in fine needle core biopsies. Thus, we performed a urinalysis study to search for HCMV-specific RNA transcripts in the urine sediment of patients with acute kidney injury. Methods Urinary sediment of 90 patients with acute kidney injury (AKI), including 48 renal transplant recipients (RTX) and 42 non-transplant recipients (nRTX), was collected from morning urine for RNA extraction and reverse transcription. The copy number of HCMV transcripts was evaluated using a UL132 HCMV-specific probe set and by real-time quantitative polymerase chain reaction (RT-qPCR). Results Of the 48 RTX patients, ten showed HCMV copies in their urine sediment cells. Within this group, three recipients had negative HCMV serology and received an allograft from an HCMV-seropositive donor. In addition, all three RTX patients on a belatacept-based immunosuppressive regimen had HCMV transcripts in their urine. Of the 42 nRTX patients, only two had detectable HCMV transcripts in urine sediment cells and both were under immunosuppression. Conclusions Ten immunosuppressed renal allograft recipients and two immunosuppressed non-transplant patients with AKI showed HCMV copies in urine sediment. Thus, HCMV positivity in urinary sediment appears to be associated with immunosuppression. This study describes a novel noninvasive method for detection of HCMV in urinary sediment. Whether all HCMV infections can be detected or only those with viral replication warrants further investigation.

Class II antigen expression of epidermal Langerhans cells in renal allograft recipients

1988 ◽  
Vol 1 (1) ◽  
pp. 186-189 ◽  
Author(s):  
Mats Jontell ◽  
Håkan Gäbel ◽  
Sven-Christer Öhman ◽  
Hans Brynger
Keyword(s):  
Langerhans Cells ◽  
Renal Allograft ◽  
Antigen Expression ◽  
Class Ii ◽  
Class Ii Antigen ◽  
Epidermal Langerhans Cells

scholarly journals The significance of cytologic examination of urine in the diagnosis of renal allograft dysfunction

2003 ◽  
Vol 60 (3) ◽  
pp. 299-304
Author(s):  
Zeljka Tatomirovic ◽  
Radojka Bokun ◽  
Ljljana Ignjatovic ◽  
Anastasija Aleksic ◽  
Vesna Skuletic ◽  
...  
Keyword(s):  
Acute Rejection ◽  
Acute Tubular Necrosis ◽  
Allograft Rejection ◽  
Renal Allograft ◽  
Acute Allograft Rejection ◽  
Urine Sediment ◽  
Cytologic Examination ◽  
Allograft Dysfunction ◽  
Tubular Necrosis ◽  
Cyclosporine Nephrotoxicity

Background. This paper presents our experience with cytologic examination of urine in diagnosing renal allograft dysfunction. Methods. The study group included 23 patients with renal allograft dysfunction, selected from 56 patients who underwent renal transplantation. Etiologic diagnosis was made according to the clinical picture, histological findings during allograft biopsy, and cytologic examination of urine. Urine sediment was obtained in cytocentrifuge and was air dried and stained with May Grunwald Giemsa. Results. Out of 23 patients with allograft dysfunction in 18 (78.3%) patient it was caused by acute rejection, and in 5 (8.9%) patients by allograft infarction, cyclosporine nephrotoxicity, acute tubular necrosis and chronic nephropathy. In eighteen patients (78.3%) cytologic examination of urine was pathologic, while in 16 (70%) clinical and histology findings coincided with urine cytology findings. Out of 18 patients with acute allograft rejection in 15 patients cytologic examination of urine coincided with acute rejection. Out of 7 patients with expressed cyclosporine nephrotoxicity, in 5 cytologic examination of urine confirmed the cause of allograft dysfunction, as well as in one of 2 patients with acute tubular necrosis. Cytologic examination of urine indicated parenchymal damage in 2 patients with reccurent disease (membranoproliferative and focal sclerosing glomerulonephritis). In 4 of 5 patients suffering from chronic rejection in a year?s monitoring period, urine sediment periodically consisted of lymphocytes, neutrophilic leucocytes, monocyte/macrophages, tubular cells and cilindres, without the predominance of any cell type. In 3 patients allograft dysfunction was caused by infective agents (bacteria, fungus cytomegalovirus). Conclusion. Cytologic examination of urine might be an alternative to histological in diagnosing acute allograft rejection and acute tubular necrosis or nephtotoxicity. Also it might indicate parenchymal disease while the importance of urine cytology in chronic allograft nephropathy needs to be investigated further.

Activation of isolated heterophils from chicks stimulated in vivo with salmonella enteritidis-immune lymphokines

1996 ◽  
Vol 54 ◽  
pp. 760-761
Author(s):  
R. B. Moyes ◽  
R. E. Droleskey ◽  
M. H. Kogut ◽  
J. R. DeLoach
Keyword(s):  
Lamina Propria ◽  
Intestinal Mucosa ◽  
Salmonella Enteritidis ◽  
Intestinal Tract ◽  
Polymorphonuclear Cells ◽  
Subclinical Infection ◽  
Egg Products ◽  
Immune Lymphokines ◽  
Organ Invasion

Salmonella enteritidis (SE) is of great concern to the poultry industry due to the organism's ability to penetrate the intestinal mucosa of the laying hen and subsequently colonize the ovaries and yolk membrane. The resultant subclinical infection can lead to SE infection of raw eggs and egg products. Interference with the ability of the organism to invade has been linked to the activation and recruitment of inflammatory polymorphonuclear cells, heterophils, to the lamina propria of the intestinal tract.Recently it has been established that heterophil activation and increased resistance to SE organ invasion can be accomplished by the administration of SE-immune lymphokines (SE-ILK) obtained from supernatants of concanavalin-A stimulated SE immune T lymphocytes from SE hyperimmunized hens. Invasion of SE into the lamina propria provides a secondary signal for directing activated heterophils to the site of SE invasion.

Childhood bullous pemphigoid associated with chronic renal allograft rejection

1998 ◽  
Vol 138 (3) ◽  
pp. 547-548 ◽  
Author(s):  
Yamazaki ◽  
Yokozeki ◽  
Katayama ◽  
Komai ◽  
Hashimoto ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Allograft Rejection ◽  
Renal Allograft ◽  
Renal Allograft Rejection

NOVEL SURVEILLANCE AND CURE OF A DONOR‐ TRANSMITTED LYMPHOMA IN A RENAL ALLOGRAFT RECIPIENT

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A111-A111
Author(s):  
Herzig Ka ◽  
Falk Mc ◽  
Jonsson Jr ◽  
Axelsen Ra ◽  
Griffin Ad ◽  
...  
Keyword(s):  
Renal Allograft ◽  
Renal Allograft Recipient
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