scholarly journals Development and Validation of Low-Intensity Pulsed Ultrasound Systems for Highly Controlled in vitro Cell Stimulation

Ultrasonics ◽  
2021 ◽  
pp. 106495
Author(s):  
F. Fontana ◽  
F. Iberite ◽  
A. Cafarelli ◽  
A. Aliperta ◽  
G. Baldi ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Cell Stimulation ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Development And Validation

scholarly journals Low-Intensity Pulsed Ultrasound Promotes Autophagy-Mediated Migration of Mesenchymal Stem Cells and Cartilage Repair

2021 ◽  
Vol 30 ◽  
pp. 096368972098614
Author(s):  
Peng Xia ◽  
Xinwei Wang ◽  
Qi Wang ◽  
Xiaoju Wang ◽  
Qiang Lin ◽  
...  
Keyword(s):  
Cartilage Repair ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Histopathological Analysis ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Autophagy Inhibitor

Mesenchymal stem cell (MSC) migration is promoted by low-intensity pulsed ultrasound (LIPUS), but its mechanism is unclear. Since autophagy is known to regulate cell migration, our study aimed to investigate if LIPUS promotes the migration of MSCs via autophagy regulation. We also aimed to investigate the effects of intra-articular injection of MSCs following LIPUS stimulation on osteoarthritis (OA) cartilage. For the in vitro study, rat bone marrow-derived MSCs were treated with an autophagy inhibitor or agonist, and then they were stimulated by LIPUS. Migration of MSCs was detected by transwell migration assays, and stromal cell-derived factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR4) protein levels were quantified. For the in vivo study, a rat knee OA model was generated and treated with LIPUS after an intra-articular injection of MSCs with autophagy inhibitor added. The cartilage repair was assessed by histopathological analysis and extracellular matrix protein expression. The in vitro results suggest that LIPUS increased the expression of SDF-1 and CXCR4, and it promoted MSC migration. These effects were inhibited and enhanced by autophagy inhibitor and agonist, respectively. The in vivo results demonstrate that LIPUS significantly enhanced the cartilage repair effects of MSCs on OA, but these effects were blocked by autophagy inhibitor. Our results suggest that the migration of MSCs was enhanced by LIPUS through the activation autophagy, and LIPUS improved the protective effect of MSCs on OA cartilage via autophagy regulation.

Low-Intensity Pulsed Ultrasound Stimulates Osteogenic Differentiation of Periosteal Cells In Vitro

2020 ◽  
Author(s):  
Wai Myo Maung ◽  
Hidemi Nakata ◽  
Motoi Miura ◽  
Munemitsu Miyasaka ◽  
You-Kyoung Kim ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Periosteal Cells

A Focused Low-Intensity Pulsed Ultrasound (FLIPUS) System for Cell Stimulation: Physical and Biological Proof of Principle

2016 ◽  
Vol 63 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Regina Puts ◽  
Karen Ruschke ◽  
Thomas H. Ambrosi ◽  
Anke Kadow-Romacker ◽  
Petra Knaus ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Cell Stimulation ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Proof Of Principle

scholarly journals 174 THE EFFECT OF LOW-INTENSITY PULSED ULTRASOUND FOR SACFFOLD-FREE CHONDROCYTE PLATE IN VITRO AND IN VIVO

2009 ◽  
Vol 17 ◽  
pp. S102-S103
Author(s):  
K. Uenaka ◽  
S. Imai ◽  
S. Shioji ◽  
K. Kumagai ◽  
N. Okumura ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low Intensity Pulsed Ultrasound for Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1488
Author(s):  
Colleen McCarthy ◽  
Gulden Camci-Unal
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Mechanical Stimulation ◽  
Synergistic Effects ◽  
In Vivo Studies ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

As explained by Wolff’s law and the mechanostat hypothesis, mechanical stimulation can be used to promote bone formation. Low intensity pulsed ultrasound (LIPUS) is a source of mechanical stimulation that can activate the integrin/phosphatidylinositol 3-OH kinase/Akt pathway and upregulate osteogenic proteins through the production of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). This paper analyzes the results of in vitro and in vivo studies that have evaluated the effects of LIPUS on cell behavior within three-dimensional (3D) titanium, ceramic, and hydrogel scaffolds. We focus specifically on cell morphology and attachment, cell proliferation and viability, osteogenic differentiation, mineralization, bone volume, and osseointegration. As shown by upregulated levels of alkaline phosphatase and osteocalcin, increased mineral deposition, improved cell ingrowth, greater scaffold pore occupancy by bone tissue, and superior vascularization, LIPUS generally has a positive effect and promotes bone formation within engineered scaffolds. Additionally, LIPUS can have synergistic effects by producing the piezoelectric effect and enhancing the benefits of 3D hydrogel encapsulation, growth factor delivery, and scaffold modification. Additional research should be conducted to optimize the ultrasound parameters and evaluate the effects of LIPUS with other types of scaffold materials and cell types.

scholarly journals 487 THE EFFECT OF LOW-INTENSITY PULSED ULTRASOUND FOR FORMING OF SCAFFOLD-FREE CARTILAGE TISSUE IN VITRO

2008 ◽  
Vol 16 ◽  
pp. S210
Author(s):  
K. Uenaka ◽  
S. Imai ◽  
K. Ando ◽  
S. Shioji ◽  
Y. Matsusue
Keyword(s):  
Cartilage Tissue ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity
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