Corrigendum to “Comparison of measles virus-specific antibody titres as measured by enzyme-linked immunosorbent assay and virus neutralisation assay [Vaccine 21 (2003) 4210–4214]

Vaccine ◽  
2004 ◽  
Vol 22 (3-4) ◽  
pp. 544
Author(s):  
Susan van den Hof ◽  
Arianne B van Gageldonk-Lafeber ◽  
Robert S van Binnendijk ◽  
Pieter G.M van Gageldonk ◽  
Guy A.M Berbers
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Measles Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Titres ◽  
Virus Specific Antibody

scholarly journals Detection of Pseudorabies Virus Infection in Subunit-Vaccinated and Nonvaccinated Pigs using a Nucleocapsid-Based Enzyme-Linked Immunosorbent Assay

1992 ◽  
Vol 4 (2) ◽  
pp. 164-169 ◽  
Author(s):  
M. J. McGinley ◽  
D. L. Todd ◽  
H. T. Hill ◽  
K. B. Platt
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Nucleocapsid Protein ◽  
Pseudorabies Virus ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Assay ◽  
Virus Specific Antibody ◽  
Positive Threshold ◽  
Virus Exposure

The potential of a pseudorabies virus (PRV) nucleocapsid protein (NC)-based enzyme-linked immunosorbent assay (ELISA) as a screening assay for PRV infection in subunit-vaccinated and nonvaccinated pigs was studied. The NC-ELISA compared favorably to a commercial ELISA for detecting PRV infection in nonvaccinated pigs. Virus-specific antibody was first detected by the NC-ELISA between days 14 and 21 in 5 pigs challenged intranasally with 104PFU of virus. Antibody continued to be detected in these pigs through day 42, when the experiment was terminated. The NC-ELISA also detected antibody in 23 of 24 pigs from PRV-infected herds. In contrast, the commercial ELISA detected antibody 1 week earlier than the NC-ELISA in experimentally infected pigs but failed to detect antibody in 3 naturally exposed pigs that were identified by the NC-ELISA. Infection in these animals was confirmed by radioimmunoprecipitation analysis. The potential usefulness of the NC-ELISA for detecting infection in vaccinated pigs was also evaluated. The nucleocapsid-specific antibody responses of 10 PRV envelope glycoprotein subunit-vaccinated pigs were monitored prior to and following nasal exposure to a low dose (1023PFU) of PRV. Sera were collected periodically for 113 days after infection. Nucleocapsid-specific antibody responses measured by the NC-ELISA remained below the positive threshold before challenge but increased dramatically following virus exposure. Maximum ELISA responses were obtained on day 32 postchallenge (p.c.). Mean ELISA responses decreased thereafter but remained well above the positive threshold on day 113 p.c. PRV nucleocapsid protein can be used effectively as antigen in the ELISA for detecting PRV infection in both nonvaccinated and subunit-vaccinated pigs.

scholarly journals SERO-PREVALENCE OF INFECTIOUS BRONCHITIS IN CHICKEN IN BANGLADESH

1970 ◽  
Vol 7 (1) ◽  
pp. 249-252 ◽  
Author(s):  
S.K. Das ◽  
M.S.R. Khan ◽  
M. Das
Keyword(s):  
Specific Antibody ◽  
Infectious Bronchitis Virus ◽  
Age Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poultry Farm ◽  
Diagnostic Laboratory ◽  
Infectious Bronchitis ◽  
Antibody Titres ◽  
Virus Specific Antibody ◽  
Sera Samples

The investigation was conducted to detect infectious bronchitis (IB) virus-specific antibody in chicken from some selected areas of Bangladesh. The indirect enzyme linked immunosorbent assay (iELISA) was performed to estimate the infectious bronchitis virus specific antibody. In case of sera-samples collected from BRAC diagnostic laboratory, Gazipur, 100% sera samples were found to be positive to IBV; the highest antibody titres were recorded as 9867.29. The average antibody titre of samples from Anis Poultry Farm, Pabna and farms driven by beneficiary of SDC, Faridpur and layer chickens reared with the help of BEES, Hobigonj were recorded as 1234.38, 1076.94 and 4572.85 and percent of sero-positive cases were 56.67%, 43.33% and 92.50% respectively. In this study all breeds of chicken (non descriptive indigenous and commercial) and age groups were found equally susceptible to IB.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for Immunoglobulin M Antibodies against Measles Virus

2003 ◽  
Vol 10 (3) ◽  
pp. 439-442 ◽  
Author(s):  
F. Roodbari ◽  
M. H. Roustai ◽  
A. Mostafaie ◽  
H. Soleimanjdahi ◽  
R. Sarrami Foroshani ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Neutralizing Antibodies ◽  
Measles Virus ◽  
Clinical Symptoms ◽  
Maculopapular Rash ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Vaccination Programs ◽  
Elisa System

ABSTRACT Measles is a highly contagious respiratory virus infection, with typical clinical symptoms including maculopapular rash, fever, cough, coryza, and conjunctivitis. Despite implementation of widespread vaccination programs throughout the world, the rates of global morbidity and mortality are still considerable. This study was performed to design a reliable indirect enzyme-linked immunosorbent assay (ELISA) to measure measles-specific immunoglobulin M (IgM). First, human IgM was purified, and then an anti-IgM antibody was produced in rabbits and purified in a multistep process. The rabbit IgG against human IgM was conjugated with peroxidase. Measles virus-infected Vero cells produced viral antigen. One hundred serum samples from infants of 9 to 18 months of age, mostly vaccinated, were evaluated for determining the presence of specific IgM antibodies against measles virus. The samples were also evaluated for neutralizing antibodies against measles virus by a microneutralization test (MNT). By comparing the results of the ELISA with those of MNT, it was demonstrated that ELISA had a sensitivity and specificity of 100 and 92%, respectively. On the other hand, when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit (EIAgen; Adaltis Italia SPa, Bologna, Italy), a high level of agreement was shown (k = 0.926).

scholarly journals Measles Virus-Specific Antibody Levels in Individuals in Argentina Who Received a One-Dose Vaccine

2006 ◽  
Vol 44 (8) ◽  
pp. 2733-2738 ◽  
Author(s):  
M. H. Arguelles ◽  
M. L. Orellana ◽  
A. A. Castello ◽  
G. A. Villegas ◽  
M. Masini ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Measles Virus ◽  
Virus Specific Antibody ◽  
Antibody Levels ◽  
Dose Vaccine

Measles virus specific antibody in infants in a highly vaccinated society

1991 ◽  
Vol 33 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Linda W-L. Chui ◽  
Raymond G. Marusyk ◽  
Henry F. Pabst
Keyword(s):  
Specific Antibody ◽  
Measles Virus ◽  
Virus Specific Antibody
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