Hemagglutinin-specific antibody responses in immunoglobulin G, A, and M isotypes as measured by enzyme-linked immunosorbent assay after primary or secondary infection of humans with influenza A virus.

We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360–739), and RΔ6 (aa 451–551) and/or RΔ8 (aa 631–739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.

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Heterogeneous Antibody Responses in Tuberculosis

Infection and Immunity ◽

10.1128/iai.66.8.3936-3940.1998 ◽

1998 ◽

Vol 66 (8) ◽

pp. 3936-3940 ◽

Cited By ~ 162

Author(s):

Konstantin Lyashchenko ◽

Roberto Colangeli ◽

Michel Houde ◽

Hamdan Al Jahdali ◽

Dick Menzies ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Antibody Response ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Antigen Recognition ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Protein Antigens ◽

Tuberculosis Patients ◽

Specific Antibodies

ABSTRACT Antibody responses during tuberculosis were analyzed by an enzyme-linked immunosorbent assay with a panel of 10 protein antigens of Mycobacterium tuberculosis. It was shown that serum immunoglobulin G antibodies were produced against a variety of M. tuberculosis antigens and that the vast majority of sera from tuberculosis patients contained antibodies against one or more M. tuberculosis antigens. The number and the species of serologically reactive antigens varied greatly from individual to individual. In a given serum, the level of specific antibodies also varied with the antigen irrespective of the total number of antigens recognized by that particular serum. These findings indicate that person-to-person heterogeneity of antigen recognition, rather than recognition of particular antigens, is a key attribute of the antibody response in tuberculosis.

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Limitations of Pneumovax® as a detection antigen in the measurement of serotype-specific antibodies by enzyme-linked immunosorbent assay

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1258/000456302760042164 ◽

2002 ◽

Vol 39 (4) ◽

pp. 398-403 ◽

Cited By ~ 4

Author(s):

ZM Huo ◽

J Miles ◽

PG Riches ◽

T Harris

Keyword(s):

Immunosorbent Assay ◽

Specific Antibody ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Carbohydrate Antigens ◽

Specific Antibodies ◽

Elisa System ◽

Background Measurement ◽

Polysaccharide Antigens ◽

The Individual

Background: Measurement of antibody responses to polysaccharide antigens is regarded as an important assessment of an individual's ability to respond to carbohydrate antigens. The currently used assays for the measurement of pneumococcal-specific antibody use the multi-serotype vaccine Pneumovax® as the detection antigen. Methods: An equal potency enzyme-linked immunosorbent assay (ELISA) system was used to compare the measurement of serotype-specific antibody with the multi-serotype assay. Results: Our results show that the concentration of specific antibody to Pneumovax is not related to the concentration of antibody to the individual serotypes. Neither is any correlation found between the antibody concentrations to any of the three single serotypes investigated, to the mixture of the three serotypes or to Pneumovax. Conclusion: We conclude that the measurement of the concentration of the specific antibody to the mixed serotypes present in Pneumovax has serious limitations when used to evaluate the protection acquired from Pneumovax immunization against any specific serotype.

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Detection of Pseudorabies Virus Infection in Subunit-Vaccinated and Nonvaccinated Pigs using a Nucleocapsid-Based Enzyme-Linked Immunosorbent Assay

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063879200400208 ◽

1992 ◽

Vol 4 (2) ◽

pp. 164-169 ◽

Cited By ~ 3

Author(s):

M. J. McGinley ◽

D. L. Todd ◽

H. T. Hill ◽

K. B. Platt

Keyword(s):

Immunosorbent Assay ◽

Specific Antibody ◽

Nucleocapsid Protein ◽

Pseudorabies Virus ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Screening Assay ◽

Virus Specific Antibody ◽

Positive Threshold ◽

Virus Exposure

The potential of a pseudorabies virus (PRV) nucleocapsid protein (NC)-based enzyme-linked immunosorbent assay (ELISA) as a screening assay for PRV infection in subunit-vaccinated and nonvaccinated pigs was studied. The NC-ELISA compared favorably to a commercial ELISA for detecting PRV infection in nonvaccinated pigs. Virus-specific antibody was first detected by the NC-ELISA between days 14 and 21 in 5 pigs challenged intranasally with 104PFU of virus. Antibody continued to be detected in these pigs through day 42, when the experiment was terminated. The NC-ELISA also detected antibody in 23 of 24 pigs from PRV-infected herds. In contrast, the commercial ELISA detected antibody 1 week earlier than the NC-ELISA in experimentally infected pigs but failed to detect antibody in 3 naturally exposed pigs that were identified by the NC-ELISA. Infection in these animals was confirmed by radioimmunoprecipitation analysis. The potential usefulness of the NC-ELISA for detecting infection in vaccinated pigs was also evaluated. The nucleocapsid-specific antibody responses of 10 PRV envelope glycoprotein subunit-vaccinated pigs were monitored prior to and following nasal exposure to a low dose (1023PFU) of PRV. Sera were collected periodically for 113 days after infection. Nucleocapsid-specific antibody responses measured by the NC-ELISA remained below the positive threshold before challenge but increased dramatically following virus exposure. Maximum ELISA responses were obtained on day 32 postchallenge (p.c.). Mean ELISA responses decreased thereafter but remained well above the positive threshold on day 113 p.c. PRV nucleocapsid protein can be used effectively as antigen in the ELISA for detecting PRV infection in both nonvaccinated and subunit-vaccinated pigs.

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