Porcine reproductive and respiratory syndrome virus expressing E2 of classical swine fever virus protects pigs from a lethal challenge of highly-pathogenic PRRSV and CSFV

Vaccine ◽  
2018 ◽  
Vol 36 (23) ◽  
pp. 3269-3277 ◽  
Author(s):  
Fei Gao ◽  
Yifeng Jiang ◽  
Guoxin Li ◽  
Yanjun Zhou ◽  
Lingxue Yu ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Lethal Challenge ◽  
Highly Pathogenic ◽  
Highly Pathogenic Prrsv

A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

2015 ◽  
Vol 18 (4) ◽  
pp. 715-723 ◽  
Author(s):  
L. Hu ◽  
X.Y. Lin ◽  
Z.X. Yang ◽  
X.P. Yao ◽  
G.L. Li ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
Simultaneous Detection ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Multiple Infections ◽  
Highly Pathogenic

Abstract In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×104, 1.50×103, 2.10×103, 1.30×103 and 8.97×102 copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

scholarly journals The E2 Glycoprotein of Classical Swine Fever Virus Is a Virulence Determinant in Swine

2005 ◽  
Vol 79 (6) ◽  
pp. 3787-3796 ◽  
Author(s):  
G. R. Risatti ◽  
M. V. Borca ◽  
G. F. Kutish ◽  
Z. Lu ◽  
L. G. Holinka ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Vaccine Virus ◽  
Fever Virus ◽  
Genetic Determinants ◽  
Highly Pathogenic ◽  
Virus Shedding ◽  
E2 Glycoprotein ◽  
Viral Virulence

ABSTRACT To identify genetic determinants of classical swine fever virus (CSFV) virulence and host range, chimeras of the highly pathogenic Brescia strain and the attenuated vaccine strain CS were constructed and evaluated for viral virulence in swine. Upon initial screening, only chimeras 138.8v and 337.14v, the only chimeras containing the E2 glycoprotein of CS, were attenuated in swine despite exhibiting unaltered growth characteristics in primary porcine macrophage cell cultures. Additional viral chimeras were constructed to confirm the role of E2 in virulence. Chimeric virus 319.1v, which contained only the CS E2 glycoprotein in the Brescia background, was markedly attenuated in pigs, exhibiting significantly decreased virus replication in tonsils, a transient viremia, limited generalization of infection, and decreased virus shedding. Chimeras encoding all Brescia structural proteins in a CS genetic background remained attenuated, indicating that additional mutations outside the structural region are important for CS vaccine virus attenuation. These results demonstrate that CS E2 alone is sufficient for attenuating Brescia, indicating a significant role for the CSFV E2 glycoprotein in swine virulence.

scholarly journals Effectiveness of the E2-Classical Swine Fever Virus Recombinant Vaccine Produced and Formulated within Whey from Genetically Transformed Goats

2014 ◽  
Vol 21 (12) ◽  
pp. 1628-1634 ◽  
Author(s):  
O. Sánchez ◽  
M. Barrera ◽  
O. Farnós ◽  
N. C. Parra ◽  
E. R. Salgado ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Adverse Reactions ◽  
Clinical Signs ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Minor Amount ◽  
Lethal Challenge ◽  
Promising Alternative ◽  
A Minor

ABSTRACTSubunit recombinant vaccines against classical swine fever virus (CSFV) are a promising alternative to overcome practical and biosafety issues with inactivated vaccines. One of the strategies in evaluation under field conditions is the use of a new marker E2-based vaccine produced in the milk of adenovirally transduced goats. Previously we had demonstrated the efficacy of this antigen, which conferred early protection and long-lasting immunity in swine against CSFV infection. Here, we have used a simpler downstream process to obtain and formulate the recombinant E2 glycoprotein expressed in the mammary gland. The expression levels reached approximately 1.7 mg/ml, and instead of chromatographic separation of the antigen, we utilized a clarification process that eliminates the fat content, retains a minor amount of caseins, and includes an adenoviral inactivation step that improves the biosafety of the final formulation. In a vaccination and challenge experiment in swine, different doses of the E2 antigen contained within the clarified whey generated an effective immune response of neutralizing antibodies that protected all of the animals against a lethal challenge with CSFV. During the immunization and after challenge, the swine were monitored for adverse reactions related to the vaccine or symptoms of CSF, respectively. No adverse reactions or clinical signs of disease were observed in vaccinated animals, in which no replication of CSFV could be detected after challenge. Overall, we consider that the simplicity of the procedures proposed here is a further step toward the introduction and implementation of a commercial subunit vaccine against CSF.

scholarly journals Generation and Efficacy Evaluation of a Recombinant Pseudorabies Virus Variant Expressing the E2 Protein of Classical Swine Fever Virus in Pigs

2015 ◽  
Vol 22 (10) ◽  
pp. 1121-1129 ◽  
Author(s):  
Yimin Wang ◽  
Jin Yuan ◽  
Xin Cong ◽  
Hua-Yang Qin ◽  
Chun-Hua Wang ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Pseudorabies Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Complete Protection ◽  
Efficacy Evaluation ◽  
Lethal Challenge ◽  
Recombinant Pseudorabies Virus

ABSTRACTClassical swine fever (CSF) is an economically important infectious disease of pigs caused by classical swine fever virus (CSFV). Pseudorabies (PR), which is caused by pseudorabies virus (PRV), is another important infectious disease of pigs and other animals. Coinfections of pigs with PRV and CSFV occur occasionally in the field. The modified live vaccine Bartha-K61 strain has played an important role in the control of PR in many countries, including China. Since late 2011, however, increasing PR outbreaks caused by an emerging PRV variant have been reported in Bartha-K61-vaccinated swine populations on many farms in China. Previously, we generated a gE/gI-deleted PRV (rPRVTJ-delgE) based on this PRV variant, which was shown to be safe and can provide rapid and complete protection against lethal challenge with the PRV variant in pigs. Here, we generated a new recombinant PRV variant expressing the E2 gene of CSFV (rPRVTJ-delgE/gI-E2) and evaluated its immunogenicity and efficacy in pigs. The results showed that rPRVTJ-delgE/gI-E2 was safe for pigs, induced detectable anti-PRV and anti-CSFV neutralizing antibodies, and provided complete protection against the lethal challenge with either the PRV TJ strain or the CSFV Shimen strain. The data indicate that rPRVTJ-delgE/gI-E2 is a promising candidate bivalent vaccine against PRV and CSFV coinfections.

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