Prophylactic and therapeutic vaccine against Pseudomonas aeruginosa keratitis using bacterial membrane vesicles

Vaccine ◽  
2021 ◽  
Author(s):  
Saori Ito ◽  
Jutaro Nakamura ◽  
Michiko Fukuta ◽  
Takehiro Ura ◽  
Takeshi Teshigawara ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Therapeutic Vaccine ◽  
Membrane Vesicles ◽  
Bacterial Membrane

scholarly journals Active Transport in Isolated Bacterial Membrane Vesicles

1972 ◽  
Vol 247 (1) ◽  
pp. 298-304 ◽  
Author(s):  
Steven A. Short ◽  
David C. White ◽  
H. Ronald Kaback
Keyword(s):  
Active Transport ◽  
Membrane Vesicles ◽  
Bacterial Membrane

RNA release via membrane vesicles in Pseudomonas aeruginosa PAO1 is associated with the growth phase

2021 ◽  
Author(s):  
Carla Pérez‐Cruz ◽  
Ferran Briansó ◽  
Elisabeth Sonnleitner ◽  
Udo Bläsi ◽  
Elena Mercadé
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Phase ◽  
Membrane Vesicles ◽  
Pseudomonas Aeruginosa Pao1

Immunomodulatory roles and novel applications of bacterial membrane vesicles

2021 ◽  
Vol 134 ◽  
pp. 72-85 ◽  
Author(s):  
William J. Gilmore ◽  
Ella L. Johnston ◽  
Lauren Zavan ◽  
Natalie J. Bitto ◽  
Maria Kaparakis-Liaskos
Keyword(s):  
Membrane Vesicles ◽  
Bacterial Membrane ◽  
Novel Applications

scholarly journals Mechanisms of Active Transport in Isolated Bacterial Membrane Vesicles

1973 ◽  
Vol 248 (10) ◽  
pp. 3551-3565 ◽  
Author(s):  
Frank J. Lombardi ◽  
John P. Reeves ◽  
H. Ronald Kaback
Keyword(s):  
Active Transport ◽  
Membrane Vesicles ◽  
Bacterial Membrane

Transport Studies in Bacterial Membrane Vesicles

Science ◽  
1974 ◽  
Vol 186 (4167) ◽  
pp. 882-892 ◽  
Author(s):  
H. R. Kaback
Keyword(s):  
Membrane Vesicles ◽  
Bacterial Membrane ◽  
Transport Studies

Recombinant Pseudomonas bio-nanoparticles induce protection against pneumonic Pseudomonas aeruginosa infection

2021 ◽  
Author(s):  
Peng Li ◽  
Xiuran Wang ◽  
Xiangwan Sun ◽  
Jesse Cimino ◽  
Ziqiang Guan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fusion Gene ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Wild Type ◽  
Generation Vaccine ◽  
Pseudomonas Aeruginosa Infection ◽  
Reduced Toxicity ◽  
Opsonophagocytic Killing ◽  
High Immunogenicity

To develop an effective Pseudomonas aeruginosa (PA) outer-membrane-vesicles (OMVs) vaccine, we eliminated multiple virulence factors from a wild-type P. aeruginosa PA103 strain (PA103) to generate a recombinant strain, PA-m14. The PA-m14 strain was tailored with a pSMV83 plasmid encoding the pcrV-hitA T fusion gene to produce OMVs. The recombinant OMVs enclosed increased amounts of PcrV-HitA T bivalent antigen (PH) (termed OMV-PH) and exhibited reduced toxicity compared to the OMVs from PA103. Intramuscular vaccination with OMV-PH from PA-m14(pSMV83) afforded 70% protection against intranasal challenge with 6.5 × 10 6 CFU (∼30 LD 50 ) of PA103, while immunization using OMVs without the PH antigen (termed OMV-NA) or the PH antigen alone failed to offer effective protection against the same challenge. Further immune analysis showed that the OMV-PH immunization significantly stimulated potent antigen-specific humoral and T-cell (Th1/Th17) responses in comparison to the PH or OMV-NA immunization in mice, which can effectively hinder PA infection. Undiluted anti-sera from OMV-PH-immunized mice displayed significant opsonophagocytic killing of WT PA103 compared to antisera from PH antigen- or OMV-NA-immunized mice. Moreover, the OMV-PH immunization afforded significant antibody-indentpednet cross-protection to mice against PAO1 and a clinical isolate AMC-PA10 strains. Collectively, the recombinant PA OMV delivering the PH bivalent antigen exhibits high immunogenicity and would be a promising next-generation vaccine candidate against PA infection.

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