Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4)

2007 ◽  
Vol 123 (1-3) ◽  
pp. 93-103 ◽  
Author(s):  
Ibrahim S. Diallo ◽  
Glen Hewitson ◽  
Lucia L. Wright ◽  
Mark A. Kelly ◽  
Barry J. Rodwell ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Equid Herpesvirus ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals New Real-Time PCR Assay Using Allelic Discrimination for Detection and Differentiation of Equine Herpesvirus-1 Strains with A2254and G2254Polymorphisms

2012 ◽  
Vol 50 (6) ◽  
pp. 1981-1988 ◽  
Author(s):  
Kathryn L. Smith ◽  
Yanqiu Li ◽  
Patrick Breheny ◽  
R. Frank Cook ◽  
Pamela J. Henney ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Equine Herpesvirus ◽  
Pcr Assay ◽  
Allelic Discrimination ◽  
Equine Herpesvirus 1 ◽  
Herpesvirus 1

Development of a real-time PCR for the detection and quantitation of caprine herpesvirus 1 in goats

2008 ◽  
Vol 148 (1-2) ◽  
pp. 155-160 ◽  
Author(s):  
Gabriella Elia ◽  
Elvira Tarsitano ◽  
Michele Camero ◽  
Anna Lucia Bellacicco ◽  
Domenico Buonavoglia ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Herpesvirus 1

scholarly journals Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR

2018 ◽  
Vol 257 ◽  
pp. 1-6 ◽  
Author(s):  
Laxmi Narayan Sarangi ◽  
Thodangala Naveena ◽  
Samir Kumar Rana ◽  
Kota Sri Naga Leela Surendra ◽  
Rachamreddy Venkata Chandrasekhar Reddy ◽  
...  
Keyword(s):  
Real Time ◽  
Filter Paper ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: Efficacy of decitabine and valganciclovir alone or in combination

2020 ◽  
Vol 183 ◽  
pp. 104931 ◽  
Author(s):  
Côme Thieulent ◽  
Erika S. Hue ◽  
Gabrielle Sutton ◽  
Christine Fortier ◽  
Patrick Dallemagne ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Assay ◽  
Antiviral Compounds ◽  
Equid Herpesvirus ◽  
Herpesvirus 1

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen

2008 ◽  
Vol 126 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Ring Trial ◽  
Herpesvirus 1

scholarly journals Comparison of a TaqMan real-time polymerase chain reaction assay with a loop-mediated isothermal amplification assay for detection of Gallid herpesvirus 1

2011 ◽  
Vol 24 (1) ◽  
pp. 138-141 ◽  
Author(s):  
Shan-Chia Ou ◽  
Joseph J. Giambrone ◽  
Kenneth S. Macklin
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Real Time Pcr ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Chain Reaction ◽  
Loop Mediated Isothermal Amplification ◽  
Gallid Herpesvirus ◽  
Herpesvirus 1 ◽  
Polymerase Chain

A TaqMan real-time polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) assay were developed to detect Gallid herpesvirus 1 (GaHV-1, formerly Infectious laryngotracheitis virus). The standard curve of real-time PCR was established, and the sensitivity reached 10 copies/μl. In the current study, the conversion between viral titer and GaHV-1 genomic copy number was constructed. Six primers for LAMP assay amplified target gene at 65°C within 45 min, and the detection limit was 60 copies/μl. The 6 primers were highly specific, sensitive, and reproducible for detection of GaHV-1. Although the sensitivity of LAMP was lower than that of real-time PCR, LAMP was faster, less expensive, and did not require a thermocycler. The LAMP assay would be a viable alternative assay in diagnostic laboratories that do not employ real-time PCR technology.

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