Molecular characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks

2007 ◽  
Vol 146 (1-2) ◽  
pp. 148-157 ◽  
Author(s):  
Min Liao ◽  
Jinlin Zhou ◽  
Takeshi Hatta ◽  
Rika Umemiya ◽  
Takeharu Miyoshi ◽  
...  
2004 ◽  
Vol 34 (8) ◽  
pp. 799-808 ◽  
Author(s):  
Takeharu Miyoshi ◽  
Naotoshi Tsuji ◽  
M Khyrul Islam ◽  
Tsugihiko Kamio ◽  
Kozo Fujisaki

2016 ◽  
Vol 25 (3) ◽  
pp. 317-326 ◽  
Author(s):  
Bárbara Guimarães Csordas ◽  
Marcos Valério Garcia ◽  
Rodrigo Casquero Cunha ◽  
Poliana Fernanda Giachetto ◽  
Isabella Maiumi Zaidan Blecha ◽  
...  

Abstract The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplus haplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes.


2013 ◽  
Vol 75 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Adrian Patalinghug YBAÑEZ ◽  
Thillaiampalam SIVAKUMAR ◽  
Rochelle Haidee Daclan YBAÑEZ ◽  
Jowarren Catingan RATILLA ◽  
Zandro Obligado PEREZ ◽  
...  

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 141
Author(s):  
Naotoshi TSUJI ◽  
Myung Jo YOO ◽  
Harue KASUGA ◽  
Takashi ISOBE ◽  
Tsugihiko KAMIO ◽  
...  

2018 ◽  
Vol 9 (6) ◽  
pp. 1606-1613 ◽  
Author(s):  
Ju Jiang ◽  
Huijuan An ◽  
John S. Lee ◽  
Monica L. O’Guinn ◽  
Heung-Chul Kim ◽  
...  

2008 ◽  
Vol 38 (5) ◽  
pp. 568-580 ◽  
Author(s):  
Eliane Esteves ◽  
Flavio A. Lara ◽  
Daniel M. Lorenzini ◽  
Gustavo H.N. Costa ◽  
Aline H. Fukuzawa ◽  
...  

Parasitology ◽  
2016 ◽  
Vol 143 (13) ◽  
pp. 1802-1809 ◽  
Author(s):  
JOSÉ MIGUEL FLORES FERNÁNDEZ ◽  
CARLA PATRICIA BARRAGÁN ÁLVAREZ ◽  
CARLA VANESSA SÁNCHEZ HERNÁNDEZ ◽  
EDUARDO PADILLA CAMBEROS ◽  
CELIA GONZÁLEZ CASTILLO ◽  
...  

SUMMARYThe cattle tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite of major importance for the livestock industry. It shows a remarkable ability to survive over long periods without feeding. However, the mechanisms used to endure long-term starvation are poorly understood. It is believed that autophagy, a process of intracellular protein degradation, may play a significant role to confront adverse environmental conditions. To advance our understanding of autophagy in R. microplus, in the present study we report the molecular characterization of three autophagy-related (ATG) genes, namely, RmATG3, RmATG4 and RmATG6, as well as their expression profiles in different developmental stages and organs of the parasite. The deduced amino acid sequences derived from the characterized gene sequences were subjected to Basic Local Alignment Search Tool analysis. The testing produced significant alignments with respective ATG proteins from Haemaphysalis longicornis and Ixodes scapularis ticks. Real-time polymerase chain reaction assays revealed that RmATG4 and RmATG6 transcripts were elevated in egg and ovary tissue, when compared with larva and midgut samples, while RmATG3 expression in midgut was 2-fold higher than in egg, larva and ovary samples.


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