scholarly journals New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

2016 ◽  
Vol 25 (3) ◽  
pp. 317-326 ◽  
Author(s):  
Bárbara Guimarães Csordas ◽  
Marcos Valério Garcia ◽  
Rodrigo Casquero Cunha ◽  
Poliana Fernanda Giachetto ◽  
Isabella Maiumi Zaidan Blecha ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Strong Support ◽  
Boophilus Microplus ◽  
The Other ◽  
Clade B ◽  
Clade C ◽  
Taxonomic Problem ◽  
Cox 1 ◽  
Brazilian Populations

Abstract The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplus haplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes.

scholarly journals First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines

2013 ◽  
Vol 75 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Adrian Patalinghug YBAÑEZ ◽  
Thillaiampalam SIVAKUMAR ◽  
Rochelle Haidee Daclan YBAÑEZ ◽  
Jowarren Catingan RATILLA ◽  
Zandro Obligado PEREZ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Anaplasma Marginale ◽  
Boophilus Microplus ◽  
Boophilus Microplus Ticks

scholarly journals Morpho-molecular characterization of Colombian and Brazilian populations of Rotylenchulus associated with Musa spp

2019 ◽  
Vol 51 ◽  
pp. 1-13 ◽  
Author(s):  
Donald Riascos-Ortiz ◽  
Ana Teresa Mosquera-Espinosa ◽  
Francia Varón De Agudelo ◽  
Claudio Marcelo Gonçalves de Oliveira ◽  
Jaime Eduardo Muñoz-Flórez
Keyword(s):  
Molecular Characterization ◽  
Musa Spp ◽  
Brazilian Populations

scholarly journals Biological and Molecular Characterization of Four Botryosphaeria Species Isolated from Pear Plants Showing Stem Wart and Stem Canker in China

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 716-726 ◽  
Author(s):  
Lifeng Zhai ◽  
Meixin Zhang ◽  
Gang Lv ◽  
Xiaoren Chen ◽  
Nana Jia ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Stem Canker ◽  
Morphological Characterization ◽  
The Other ◽  
Internal Transcribed Spacer Region ◽  
Pathogenicity Tests ◽  
The Common ◽  
Stem Cankers

Pear stem wart and pear stem canker, which have been considered as two different fungal diseases caused by pathogens belonging to Botryosphaeria spp., commonly occur and cause serious damage in the main pear-producing areas in China. To identify the species of this genus infecting pear in China, 131 Botryosphaeria isolates were recovered from pear samples exhibiting symptoms collected from 20 different provinces and areas. Morphological characterization and phylogenetic analyses of the ribosomal DNA internal transcribed spacer region and the β-tubulin and EF1-α genes revealed that Botryosphaeria dothidea, B. rhodina, B. obtusa, and B. parva were associated with different pear stem wart and stem canker symptoms. Remarkably, all isolates of B. dothidea were obtained from the samples showing either stem wart or stem canker lesions; however, the isolates of the other three species were obtained only from the samples showing stem canker. Pathogenicity tests on the pear shoots showed that B. dothidea isolates could induce stem wart or stem canker lesions but all the isolates of the other three species could only induce stem cankers. However, the isolates of B. parva, B. rhodina, and B. obtusa exhibited higher virulence than that of the B. dothidea isolates on the pear fruit. Our results suggest that B. dothidea is the common causal agent for these two diseases (a pear stem wart and a pear-related stem canker), whereas B. parva, B. rhodina, and B. obtusa only cause pear stem canker diseases. To our knowledge, this study represents the first report for biological and molecular characterization of four Botryosphaeria spp. isolated from pear plants showing stem wart and stem canker in China.

scholarly journals Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus

2008 ◽  
Vol 38 (5) ◽  
pp. 568-580 ◽  
Author(s):  
Eliane Esteves ◽  
Flavio A. Lara ◽  
Daniel M. Lorenzini ◽  
Gustavo H.N. Costa ◽  
Aline H. Fukuzawa ◽  
...  
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
Embryonic Cell ◽  
Boophilus Microplus ◽  
Embryonic Cell Line

scholarly journals Morphological and molecular characterization of Brazilian populations of Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) and the evolutionary relationship among species of Diatraea Guilding

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0186266 ◽  
Author(s):  
Fabricio J. B. Francischini ◽  
Jaqueline Bueno de Campos ◽  
Alessandro Alves-Pereira ◽  
João Paulo Gomes Viana ◽  
Christopher C. Grinter ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Evolutionary Relationship ◽  
Diatraea Saccharalis ◽  
Morphological And Molecular Characterization ◽  
Brazilian Populations

scholarly journals The First Molecular Characterization of Picocyanobacteria from the Argentine Sea

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Macarena Perez-Cenci ◽  
Gonzalo F. Caló ◽  
Ricardo I. Silva ◽  
Rubén M. Negri ◽  
Graciela L. Salerno
Keyword(s):  
Molecular Characterization ◽  
Phytoplankton Biomass ◽  
Pcr Amplification ◽  
Environmental Dna ◽  
The Other ◽  
Southwest Atlantic ◽  
Argentine Sea ◽  
The World ◽  
High Nutrient

Picocyanobacteria are abundant throughout the world’s oceans. Particularly, it has been reported thatSynechococcusstrains have a wide latitudinal distribution, from polar to tropical waters. However, their molecular characterization in the Southwest Atlantic Ocean is still missing. We analyzedSynechococcusgenetic diversity in a sector of the Argentine Sea, one of the richest biological areas of the world oceans.16S rRNAamplicons obtained after PCR amplification of environmental DNA extracted from water samples of this area were used for DGGE and sequenced. OnlySynechococcussequences could be retrieved. On the other hand, we isolated twoSynechococcusstrains from the environment. Our analyses revealed that the clade I group was widespread from latitude 38°S to 48°S and that can coexist with clade IV strains in shelf waters. The cooccurrence of these two clades may be related to an adaptation to high-nutrient/low-temperature waters. Our data are the first report onSynechococcusecotypes that would be important contributors to phytoplankton biomass in the Argentine Sea, one of the richest biological areas of the world oceans.

Phylogenetic relationships in Pterostylidinae (Cranichideae: Orchidaceae): combined evidence from nuclear ribomsomal and plastid DNA sequences

2011 ◽  
Vol 59 (2) ◽  
pp. 99 ◽  
Author(s):  
Mark A. Clements ◽  
J. Tupac Otero ◽  
Joseph T. Miller
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Signal ◽  
Strong Support ◽  
Its Region ◽  
Plastid Dna ◽  
Classification Systems ◽  
Parsimony Analysis ◽  
Internal Support ◽  
Clade B ◽  
Clade C

A study to evaluate the relationships in subtribe Pterostylidinae (Cranichideae: Orchidaceae) was undertaken using DNA sequences from the nuclear ribosomal ITS region (256 taxa) and plastid matK (subset of 37 taxa). Parsimony analysis of nuclear, plastid and combined datasets revealed that there is strong support for the monophyly of Pterostylidinae, and three major groups therein. Clades A–C contain nine, possibly 10, identifiable groups supported by morphological synapomorphies. Clade A comprises the following two major, strongly supported groups that correlate with morphological synapomorphies: (1) Speculantha (including Petrorchis) and (2) Linguella and Eremorchis, sister to an unresolved polytomy containing Taurantha, a paraphyletic Crangonorchis and polyphyletic Diplodium. There is no support for continued recognition of Taurantha, Crangonorchis, Linguella and Eremorchis, all of which are embedded within the broader, strongly supported, monophyletic Diplodium. Clade B represents true Pterostylis. Clade C contains the morphologically disparate Bunochilus, Hymenochilus, Oligochaetochilus, Pharochilum, Plumatichilos, Stamnorchis and Urochilus (including Ranorchis) in a partially resolved tree. There is strong molecular and morphological synapomorphic internal support for the recognition of these taxa as genera. Our results revealed that none of the presently proposed classification systems for Pterostylidinae truly accounts for the underlying phylogenetic signal. A streamlined classification system, therefore, seems warranted, although further research based on a larger plastid DNA dataset is required to elucidate relationships in Clade C.

scholarly journals Molecular Characterization of Salmonella Species Isolates from Some Hospitals in Jos, Nigeria

2021 ◽  
Vol 2 (2) ◽  
pp. 1-5
Author(s):  
S. B. Uneze ◽  
P. F. Chollom ◽  
Y. A. Agabi ◽  
D. J. Mawak ◽  
O. J. Egbere ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Molecular Size ◽  
Molecular Techniques ◽  
The Other ◽  
Chain Reaction ◽  
Inva Gene ◽  
Polymerase Chain ◽  
Similarities And Differences

The conventional methods of identification of Salmonella involving microbiological enrichment and successive identification mostly are tedious, time consuming and not specific. Therefore, the aim of this study was to utilize molecular techniques to characterize Salmonella species isolates from some Hospitals in Jos, Nigeria. The 10 isolates collected from some Hospitals in Jos, Nigeria were screened for Salmonella using conventional biochemical methods. The positive isolates were identified using polymerase chain reaction (PCR) for discernment of invasion A (invA) gene at explicit molecular size (284 bp) utilizing explicit primers (forward and reverse). Sequencing of the invA gene was performed and the similarities and differences between our invA gene and published sequences on GenBank were assessed. Seven out of ten confirmed Salmonella species isolates were positive to the invA gene while the remaining three were negative. The homology level of nucleotide sequence (97.746%) demonstrated high similitude between the local isolates and the other sequences on GenBank. Molecular characterization of the Salmonella isolates provides data about the virulence of the pathogen just as its relatedness to different organisms which offer data about the genome of the organisms and are helpful for epidemiological examinations. Therefore, Molecular methods which enable the detection of virulent genes are extremely important surveillance tools that are required to assist in curbing the escalation of infections caused by Salmonella.

scholarly journals Molecular Characterization of the Complete 23F Capsular Polysaccharide Locus of Streptococcus pneumoniae

1998 ◽  
Vol 180 (19) ◽  
pp. 5273-5278 ◽  
Author(s):  
Mario Ramirez ◽  
Alexander Tomasz
Keyword(s):  
Streptococcus Pneumoniae ◽  
Molecular Characterization ◽  
Dna Sequence ◽  
Capsular Polysaccharide ◽  
Open Reading Frames ◽  
The Other ◽  
Reading Frames

ABSTRACT The complete DNA sequence of the capsular locus 23F ofStreptococcus pneumoniae is presented. The 18.6-kbcps23f locus is composed of 18 open reading frames flanked at the 5′ and 3′ ends by the genes dexB andaliA, an arrangement similar to those of some of the other identified cps loci.

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