Thermally induced early events of ribonuclease A under reducing conditions: Evidenced by principal component analysis and two-dimensional correlation infrared spectroscopy

2009 ◽  
Vol 51 (1) ◽  
pp. 4-10 ◽  
Author(s):  
Li-Xu Wang ◽  
Liping Zhang ◽  
Yuqing Wu
Keyword(s):  
Principal Component Analysis ◽  
Infrared Spectroscopy ◽  
Principal Component ◽  
Component Analysis ◽  
Ribonuclease A ◽  
Two Dimensional ◽  
Thermally Induced ◽  
Reducing Conditions

Principal Component Analysis-based Strategy to Identify the Presence of Tissue-Cultured and Wild-Type Dendrobium officinale and Dendrobium moniliforme in a Commercial Preparation

2018 ◽  
Vol 17 (4) ◽  
pp. 355-362
Author(s):  
Chen Nai-dong ◽  
Chen Yue ◽  
Zhang Li ◽  
Bao Lin-Lin ◽  
Chen Yan-Jun ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Infrared Spectroscopy ◽  
Principal Component ◽  
Component Analysis ◽  
Dendrobium Officinale ◽  
Infrared Analysis ◽  
Two Dimensional ◽  
Wild Type ◽  
Commercial Preparation ◽  
Different Ages

A principal component analysis-based two-dimensional correlation infrared spectroscopy technique combined with tri-step infrared macro-fingerprinting was explored to discriminate 1–4 years of tissue-cultured from wild-type Dendrobium officinale and Dendrobium moniliforme. The results showed that although speciation and origins of the Dendrobium samples could be differentiated by Fourier-transform infrared spectroscopy and second derivative infrared spectroscopy spectra, it is quite difficult to identify the age of Dendrobiums. In the further principal component analysis-based two-dimensional correlation infrared analysis, the range 1390–1230 cm-1 and 1180–1020 cm-1, in which the auto-peaks attributed a major variance ratio, were chosen to discriminate different cultivated years of Dendrobiums. Different ages of D. officinale and D. moniliforme showed their characteristic auto-peaks and cross-peaks with different peak positions and intensity in these two range. Our research indicated that principal component analysis-based strategy combined with tri-step infrared macro-fingerprint is suitable to rapidly discriminate the different ages of Dendrobiums with species and origins.

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