scholarly journals A Sobemovirus coat protein gene complements long-distance movement of a coat protein-null Dianthovirus

Virology ◽  
2004 ◽  
Vol 330 (1) ◽  
pp. 186-195 ◽  
Author(s):  
Anton S. Callaway ◽  
Carol G. George ◽  
Steven A. Lommel
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Long Distance ◽  
Long Distance Movement

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals The Determinant of Potyvirus Ability to Overcome the RTM Resistance of Arabidopsis thaliana Maps to the N-Terminal Region of the Coat Protein

2009 ◽  
Vol 22 (10) ◽  
pp. 1302-1311 ◽  
Author(s):  
V. Decroocq ◽  
B. Salvador ◽  
O. Sicard ◽  
M. Glasa ◽  
P. Cosson ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Coat Protein ◽  
Biological Properties ◽  
Terminal Region ◽  
Plum Pox Virus ◽  
Lettuce Mosaic Virus ◽  
Long Distance ◽  
Resistance Breaking ◽  
Viral Determinant ◽  
Long Distance Movement

In Arabidopsis thaliana Columbia (Col-0) plants, the restriction of Tobacco etch virus (TEV) long-distance movement involves at least three dominant RTM (restricted TEV movement) genes named RTM1, RTM2, and RTM3. Previous work has established that, while the RTM-mediated resistance is also effective against other potyviruses, such as Plum pox virus (PPV) and Lettuce mosaic virus (LMV), some isolates of these viruses are able to overcome the RTM mechanism. In order to identify the viral determinant of this RTM-resistance breaking, the biological properties of recombinants between PPV-R, which systemically infects Col-0, and PPV-PSes, restricted by the RTM resistance, were evaluated. Recombinants that contain the PPV-R coat protein (CP) sequence in an RTM-restricted background are able to systemically infect Col-0. The use of recombinants carrying chimeric CP genes indicated that one or more PPV resistance-breaking determinants map to the 5′ half of the CP gene. In the case of LMV, sequencing of independent RTM-breaking variants recovered after serial passages of the LMV AF199 isolate on Col-0 plants revealed, in each case, amino acid changes in the CP N-terminal region, close to the DAG motif. Taken together, these findings demonstrate that the potyvirus CP N-terminal region determines the outcome of the interaction with the RTM-mediated resistance.

scholarly journals Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

2011 ◽  
Vol 36 (2) ◽  
pp. 121-124 ◽  
Author(s):  
José Evando A. Beserra Jr. ◽  
Eduardo C. Andrade ◽  
Rosa F.R. Araújo Camarço ◽  
Aline K.Q. Nascimento ◽  
José Albérsio A. Lima
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Northeastern Brazil ◽  
Sequence Variability ◽  
Virus Isolates

scholarly journals Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil

2002 ◽  
Vol 27 (3) ◽  
pp. 279-284 ◽  
Author(s):  
OSMAR NICKEL ◽  
THOR V. M. FAJARDO ◽  
FRANCISCO J. L. ARAGÃO ◽  
CÉSAR M. CHAGAS ◽  
GILMAR B. KUHN
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Amino Acid Sequences ◽  
Grapevine Virus ◽  
Leaf Extracts ◽  
Western Blots ◽  
Gene Characterization ◽  
Gene Coding ◽  
Grapevine Virus B

An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific primers for GVB, a fragment of 594 bp, comprising the coat protein gene coding for 197 amino acids, was amplified by RT-PCR with viral RNA extracted from GVB-infected N. occidentalis. The nucleotide and the deduced amino acid sequences of the coat protein gene showed high identities with Italian and Japanese isolates of GVB.

In vitro expression of a chimeric coat protein gene from Grapevine Fanleaf virus (strain F 13)

1991 ◽  
Vol 117 (3-4) ◽  
pp. 297-304 ◽  
Author(s):  
M. A. Serghini ◽  
M. Pinck ◽  
L. Pinck
Keyword(s):  
Coat Protein ◽  
Virus Strain ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Grapevine Fanleaf Virus ◽  
In Vitro Expression

scholarly journals Ecdysone agonist‐inducible expression of a coat protein gene from tobacco mosaic virus confers viral resistance in transgenic Arabidopsis

2004 ◽  
Vol 37 (3) ◽  
pp. 439-448 ◽  
Author(s):  
Ja Choon Koo ◽  
Sebastian Asurmendi ◽  
Jennifer Bick ◽  
Terry Woodford‐Thomas ◽  
Roger N. Beachy
Keyword(s):  
Coat Protein ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transgenic Arabidopsis ◽  
Inducible Expression ◽  
Viral Resistance ◽  
Ecdysone Agonist

Coat protein gene-mediated protection in Lactuca sativa against lettuce mosaic potyvirus strains

1997 ◽  
Vol 3 (1) ◽  
pp. 75-86 ◽  
Author(s):  
Sylvie Dinant ◽  
Brigitte Maisonneuve ◽  
Josette Albouy ◽  
Yves Chupeau ◽  
Marie-Christine Chupeau ◽  
...  
Keyword(s):  
Coat Protein ◽  
Lactuca Sativa ◽  
Coat Protein Gene ◽  
Protein Gene
Sign in / Sign up

Export Citation Format

Share Document